Determining Photosynthetic Pigments using Chromatography and Spectrophotometry Photosynthesis is a widely studied topic among the world of science due to its importance for life and its many uses. Photosynthetic pigments reflect and absorb different wavelengths of visible light based off their polarity. In this experiment, we studied photosynthetic pigments, first, by determining polarity and then, by measuring the amount of light of a given wavelength that a pigment absorbs. We used two methods
Experiment 1: The Composition of Aqueous Complexes by UV-vis Spectrophotometry CHEM 4310L Analytical Chemistry II Call Section 100 Monday, Wednesday9:40-12:45 Joshua Holley, Kyle McQauid 01/22/14 Introduction The purpose of this experiment is to determine the stoichiometry of a complex ion using Job’s method. In doing so, experience is gained in using the techniques and instrumentation involved in absorbance spectrophotometry. When a metal cation (M) reacts with a ligand (L) a complex forms
Introduction: Spectrophotometry is a widely used method to calculate how much light is absorbed by a chemical substance. This is done by measuring the intensity of light as it passes through a sample solution. The principle of this method is that a compound absorbs or transmits light over a certain wavelength from which the measurement can be used to calculate the concentration of a known chemical substance. A spectrophotometer is an instrument that would measure the amount of light a sample would
Clinical Laboratory Instrument Home Assignment Student Names: 1. : Muram izzat k-217770903 2. Aisha Mohamed k-215810920 3. Rozhina Khonji k-217010901 4. Shuhd Alraba k-7176609018 Lecturer Name: Dr. Faruk Sarkinfada Task 1: A-What are the major functions of a clinical laboratory in a health care setting? There are many section in the clinical laboratory in general the clinical laboratory is responsible for analyzing patient specimens in order to provide information to aid in the diagnosis
Conclusions Experiment one was conducted to show the separation of plant pigments using a process called paper chromatography. The significance of this lab was to show different types of pigments and discover which pigments would have the highest band along the filter paper. Experiment one of the lab exhibited that chlorophyll a (figure 1) would display the highest band of pigments on the filter paper when using the paper chromatography process rather than chlorophyll b, xanthophyll and carotenoids
transcriptomic information with this proteogenomic methods and tools. Many of proteomic information can be achieved by gene prediction algorithms, cDNA sequences and comparative genomics. Large proteomic datasets can be gained by peptide mass spectrophotometry for proteogenomics because it uses proteomic data to annotate genome. If there is genome sequence data for an organism or closely related genomes are present,proteogenomic tools can be used. Gained proteogenomic data provides comparing of these
contains soluble sedimentary rocks like limestone(CaCO3), pipes with scale can reduce the water flow. This problem increases the energy bills about 25%.3 The hardness of water can be determined by many scientific techniques. Atomic Absorption Spectrophotometry(AA) is useful to determine metals dissolved in a solution. The chemical principle behind AA is the Beer-Lambert Law. It can be easily used to calculate the metal concentration in the water sample. Atoms have energy levels are unique. Atoms need
chromatography, mass spectrometry, and spectrophotometry. Microcrystalline tests are more specific than the color tests and is uses the polarizing microscope. Gas chromatography is when the sample is separated into its different components based on size and chemical structure. Mass spectrometry fragments the molecules in the sample and that pattern of fragmentation helps with the identification of the substance when compared to a known standard. Spectrophotometry identifies substances by measuring
The phenol-sulfuric acid method is a simple and rapid colorimetric method to determine total sugar in a sample. The method detects virtually all classes of carbohydrates, including mono-, di-, oligo-, and polysaccharides. It is one of the most versatile, relatively easy and cheap approaches for determination of carbohydrate concentrations is the colorimetric method based on reaction between hydrolysed carbohydrate solution and a colouring reagent that develops colour that is detectible in the visible
total of 80 members were enrolled in the study out of which 55 were breast cancer patients and 25 were healthy individuals from whom the required data and informed consent was obtained, and then serum was analyzed using diphenylamine method and spectrophotometry. Results: Serum sialic acid levels of healthy subjects were compared with that of the breast cancer patients and a large difference was found which represents very higher serum sialic acid levels of breast cancer patients and very lower levels
isolate E. coli lac operon mutants via mutant screening using MacConkey/lactose and confirmation by growth in MacConkey/maltose plates 2. To determine the genotypes of two different E. coli lac operon mutants via β-galactosidase activity assay by spectrophotometry and the results of complementation test, with the introduction of various plasmids.
volume, but its fluidity causes it to take the shape of the container in which it is residing. A gas has neither a definite shape nor volume, and it will completely fill any container into which it is place” (2011, Pg. 120). Chromatography, spectrophotometry, and mass spectrometry are used to identify or compare organic materials. As explained by Saferstein “Chromatography is a means of separating and tentatively identifying the components of a mixtur... ... middle of paper ... ...ively place
Abstract The subject of whether the Shroud of Turin is over 2000 years old bit of material fabric that really served as an internment piece of clothing for Jesus Christ or a very genius work of forgery has been a dilemma for many religious figures and scientists. Several factors affected the clarity and difficulty of finding an answer for that question. One of these factors is physical factor that had great effect on the complexity of such case as the gaps, blood stains, contortions, and burnings
Introduction When white light passes through a prism, it forms a continuous spectrum of colors found in the visible region of the electromagnetic spectrum. The color of the compound is a blend of wavelengths that are transmitted and not absorbed by the sample. Beer- Lambert’s Law is applied in order determine the concentration of a colored species in solution. The concentration of the colored species is directly proportional to it absorbance at a wavelength. A= εbc which A is the absorbance, ε is
Title Enumeration of yeast from a pure culture or diluted solution by methods of direct count, viable count, and turbidity study. Abstract The goal for using all three enumeration techniques is to not only understand direct count, viable count, and turbidity study, it is to also understand the advantages and disadvantages of each so that researchers can use them in the most efficient ways when studying microorganisms. The idea was that if the direct count gives a certain number of microorganisms
of paper ... ...in a 1:1 ratio to form a purple colored complex. In the phosphate lab you learned to use spectrophotometry to determine the concentration of an unknown. An iron solution and salicylic acid will be available, as well as pipettes and volumetric flasks. In the density lab you learned to develop your own procedure for an experiment. You should be able to use spectrophotometry to determine the amount of salicylic acid in your aspirin. You will need to create a calibration graph and use
Factors Affecting the Equilibrium Reaction of Iron (III) and Thiocyanate ions Research Question How does the change in temperature of Iron (III) Thiocyanatoiron, containing iron (III) ions Fe3+ (aq) and thiocyanate ions SCN¬¬- (aq), affect the absorbance of the solution? - Temperature at 25 °C, 30 °C, 35 °C, 40 °C, 45 °C, 50 °C, 55 °C, and 60 °C (equilibrate the Iron (III) Thiocyanatoiron (aq) in various temperatures using a digital water bath, and temperature checked using a digital thermometer
ONPG (ortho-nitro-phenyl-galactoside). ONPG has a structure similar to lactose, so it can bind to the enzyme and be cleaved. The products are galactose and ortho-nitrophenolate. ortho-nitrophenolate is yellow in color and can be measured by spectrophotometry. the rate of an enzyme can be measured. The reaction is usually expressed like this E is the enzyme, S is the substrate, ES is the complex of the two and P is the product. Each step 1) formation of ES and 2) formation of product goes forward
http://www.jenway.com/product.asp?dsl=302 Plastic Cuvettes (visible range). (n.d.). > Vernier Software & Technology. Retrieved May 23, 2014, from http://www.vernier.com/products/accessories/cuv/ Spectrophotometry. (n.d.). : Beer's Law. Retrieved May 23, 2014, from http://www.chm.davidson.edu/vce/spectrophotometry/beerslaw.html What Is a Colorimeter?. (n.d.). wiseGEEK. Retrieved May 23, 2014, from http://www.wisegeek.org/what-is-a-colorimeter.htm absorption spectra - the Beer-Lambert Law. (n.d.). absorption
In this experiment, spectrophotometry will be used to measure the rate of reaction by measuring the increasing absorbance of 430 nm light by I2, as concentration of iodine increases.1 The order of a chemical is the number by which the rate of the reaction exponentially increases with increasing concentration of the chemical, and it’s always a whole number. An order of 0 is assigned when there is no exponential increase with the increase in concentration of that chemical.2 A spectrophotometer will