of 11 test tubes were set up exactly as in the previous step. The first duplicate was series A while the second duplicate was series B and the last one was series C. Series A did not contained any inhibitors so additional water was added to each tube. Series B contained the 50mM lactose while series C contained the 150mM lactose. The amounts of buffer, ONPG, and water added to each tube in each series are listed in detailed in tables 4, 6, and 8. The Lineweaver- Burk plot of each series was created
must be removed if the cell is to remain unharmed. Method 1. Label the test tubes A, B, C, and D. label the staining dishes B, C, and D. 2. Put 5cm3 of hydrogen peroxide in each test tube. Be careful -hydrogen peroxide is dangerous. 3. Put two cubes of raw liver into another test tube and put this in the water bath (set at boiling) for 2 minutes. 4. Use forceps to remove the cubes from the test tube. Place one cube on the staining dish B. Place the second cube in the mortar.
formula for this is: Copper Carbonate = Copper Oxide + Carbon Dioxide (CuCO3 = CuO + CO2) The reactivity series determines how fast this reaction occurs. The reactivity series is the order of metals in the periodic table. The most reactive metals are placed at the top of the reactivity series. The least reactive materials are placed at the bottom of the reactivity series. From preliminary work that I have already done I know that Potassium and sodium are the most reactive metals, and
Each student in Microbiology, went through a series of experiments to determine the unknown microbe that was given. The microorganism was inoculated to different test that in 48 hours the solution would either change or remain unchanged. Though some of the experiments required additional steps that was done on the next day of lab. Not only was a series of test done, but also a gram stain was recommended during the discovery of the unknown. The gram stain played a major step, if this step was done
solvent containing a solute of interest to each of the specially designed as in Figure 1, interconnected tubes in the series, then adding a second, lighter solvent that provides a partition coefficient greater than 1 to the first tube only. The two solvents are then mixed together, then allowed to separate into layers. The lighter top solvent is then transferred to the second tube in the series by tilting the apparatus, while the heavier bottom layer is retained. A fresh quantity of the lighter
relative reactivities of some common oxidants and reductants, and then to check these predictions by experiment. APPARATUS The following materials are required in order to complete this experiment:,,X Reaction palette or set of semi-micro test tubes and stand,,X Clean plastic tray,,X Latex gloves,,X Goggles ,,X Set of small dropper bottles containing each of the following: fæ Freshly prepared FeSO4 fæ 0.1M FeCl3 solution fæ 1M sulfuric acid, H2SO4 fæ 0.1M H2O2 solution fæ 0.1M KSCN
fan) is across the tube bundle which may be either forced draft or induced draft as shown in the Figure 2.2. A support structure that high enough to allow air to enter beneath the air cooled heat exchanger. 2.4.3 Shell and tube heat exchanger Figure 2.3 Shell and tube heat exchanger From the Figure 2.3, shell and tube heat exchangers consist of a series of tubes. One set of these tubes contains the fluid that must be either heated or cooled. The second fluid runs over the tubes that are being heated
To start this study, nine labeled test tubes were setup with precise amounts of 2mL of deionized water, and 1ml of 50-50 corn syrup to water mixture. The addition of 1mL of yeast would also be added, but this will not be added until the fermentation apparatus is assembled in the water bath and ready to begin the reaction. The assemble of our apparatus included submerging and combining of the test tube and tubing with a stopper to ensure no air is in the apparatus. Then
30 min Tube A 0.042 0.323 0.592 0.717 0.830 0.892 Tube B 0.114 0.917 0.949 0.955 0.956 0.957 Tube C 0.093 0.543 0.625 0.919 0.947 0.952 Tube D 0.169 0.234 0.410 0.416 0.438 0.445 Tube E 0.044 0.057 0.106 0.115 0.201 0.227 Tube F 0.039 0.044 0.129 0.133 0.134 0.133 Tube G 0.045 0.046 0.064 0.105 0.134 0.134 Table 1 shows that the difference in absorption increases as time progresses.
of each tube was read and recorded on the Nitrophenol standard curve for acid phosphate assays
week’s experiment, in which E. Coli bacteria’s was lysed and through a series of chemical extractions it’s inner contents were harvested. Methods: 4.5 mL E.Coli EDTA suspension was pipetted into a conical tube. After this 0,25 mL lysosome solution was put inside the same tube. Both were incubated at 37°C for so minutes. Once out of the incubator, 0.5 mL of 10% SDS was added. In order to ensure a good mixing of the liquids the tube was inverted continuously for several minutes. Incubation occurred once
A pure culture of an unknown bacterium labeled # 10B test tube was handed to me by my instructor. A gram-staining kit, bibulous paper, a light microscope, inoculating needle, and API 20E system were also used. A slide was prepared with the unknown bacteria and the Gram stain using crystal violet and safranin as
track is made up of a series of hollow organs joined in a long, twisting tube from the mouth to the anus. The process of digestion requires ingestion, digestion, movement, absorption, and elimination. All parts of the tract have four layers. These layers surround the lumen, or interior space of the GI tract. Two “solid” digestive organs, discussed in the latter units of this chapter are the liver and the pancreas, produce digestive juices that reach the intestine through small tubes called ducts. The
information was a cathode ray tube. (Chang, 1998) A cathode ray tube is made of glass and does not contain any air, a vacuum. A cathode ray tube function, when two metal plates are connected, to high-voltage source, which makes the negatively charged plate, known as the cathode, emit an invisible ray. The cathode draws towards the anode (positive and negative attract towards each other), where it passes through a hole in the tube and continues towards the end of the tube. When the ray strikes the specifically
The cathode ray tube was invented in 1875 by the name of Sir Williams Crooke. Yet he wasn’t the one to make the big discovery. In 1897, a man by the name of J.J. Thompson conducted a series of experiments to prove the existence of subatomic particles. He wasn’t 100% correct with all of his claims he made but broke the theory John Dalton stated that the smallest form matter could be broken down to was an atom. Having shown the world that there was smaller than an atom, it later caused others to question
In order to make that correlation, you would need to measure a series of known dilutions of ortho-nitrophenolate, measure them with the spectrophotometer. the standard curve plotted Absorbance versus concentration for known amounts of ortho-nitrophenolate will be drawn. This will be useful in determining the rate
measure the cellular respiration in mitochondria that have been isolated from lima beans. For part one, yeast was grown in various carbohydrate solutions as a food source at various temperatures. A yeast suspension was transferred into a fermentation tube and measurements of 〖CO〗_2 produced were recorded. This Introduction: Cellular respiration is the method of breaking down organic molecules to release their stored energy. Plants and animals use cellular respiration to use energy. Aerobic respiration
In this experiment there is two parts to fulfill. One included the synthesis of tert-butyl chloride and the other consists of qualitative chemical tests for reactivity. During the synthesis portion one will perform a series of procedures to add and draw off different chemicals in a seperatory funnel to attain tert-butyl chloride. “During the qualitative chemical tests one will use silver nitrate and sodium iodide to perform SN1 and SN2 reactions with tert-butyl chloride and 1-chlorobutane” (3).
Using Various Biochemical Tests Aim To identify various unknown carbohydrates by subjecting them to a series of biochemical tests. Introduction Materials Lab coat Safety goggles Gloves 6 solid carbohydrates- Glucose, Maltose, Fructose, Starch, Sucrose, Cellulose Permanent marker Deionised water Benedict’s solution Barfoed’s reagent Diastix strips Iodine Boiled water Large beaker 12 test tubes Dimple tray Spatula Dropper pipette Experimental Method Lab coats, safety goggles, and gloves were
World Music World music is basically any type of music from all around the world. This music is generally noticeable from the other countries, either by instruments used, or lyrically it is different. These things are what make it easy to tell the difference between the different countries and what type of music it is. World music can be anything from different countries so it may sound different to how we expect music to sound like, for example gamelan music is mainly a set of tuned bronze