Molecular docking is a key tool in structural molecular biology and computer-assisted drug design. The goal of ligand—protein docking is to predict the predominant binding mode(s) of a ligand with a protein of known three-dimensional structure. Successful docking methods search high-dimensional spaces effectively and use a scoring function that correctly ranks candidate dockings. Garrett M. Morris and Marguerita Lim-Wilby, Molecular Docking, In Molecular Modeling of Proteins Methods in Molecular
towards biological sciences made me to take btech in biotechnology in jntua collage of engineering in pulivendula which is an government university. In college i have been exposed to many fields of study like biochemistry, cell biology, micro biology, molecular biology, genetics, genetic engineering, protein engineering, enz... ... middle of paper ... ...or students and also doing some research work which we will be getting from research scholars and writing articles/papers are my responsibilities During
2.2.1.2 Scatchard Plot: In order to determine the binding affinity of a ligand for its receptor, Scatchard plot analysis was done utilizing data obtained from standard titration process. r/cf vs. r was plotted as described in H. Mansouri-Torshizi and others, ‘Study on Interaction of DNA from Calf Thymus with 1,10- phenanthrolinehexyldithiocarbamatopalladium(II) Nitrate as Potential Antitumor Agent’, Journal of Biomolecular Structure and Dynamics, 28.5 (2011).based on the calculation as depicted in
The world of Model Train Building has grown greatly with the aid of computers and technology to enhance the fun of building. Technology has long been a part of Model Train building with the adding of lights, bells, and whistles to capture your interest and imagination. But with the latest generation of building comes the influx of technology and the computer. The computer brings along a new breed of builders who plan track layout, buy parts on the Internet, receive updated news, and chat with other
Background Each year snakes envenomate 421,000 people, 20,000 of whom die. These injuries are especially concentrated in developing countries, where snake bites are an occupational hazard. (Kasturiratne et al. 2008). The negative impact of this could be alleviated by the creation and production of a low-cost, human-compatible universal antivenom. Lethal Toxin Neutralizing Factor, henceforth LTNF, is a substance that has been isolated from opossum (Didelphis virginiana) serum, liquid component
Polymerase Chain Reaction (PCR) was performed to purify the DNA extract. A mastermix was needed to be made for the PCR products, the mastermix volumes were calculated and shown in table 1. PCR is a simple and inexpensive tool needed to focus on a segment of DNA and a copy it a billion times over. (2) This was needed to purify the DNA samples of the patients which were needed in a gel electrophoresis procedure. The agrose gel electrophoresis process uses electricity to separate DNA fragments by
\In-fact, mutations and genetic changes result in defence mechanisms against antimicrobials, where the majority of these changes are a direct result of selection pressure on bacteria to develop resistance.[25, 26] In addition, bacteria may obtain these genes through the process of conjugation where bacteria trade plasmids which contain resistant genes. Plasmids are an extra chromosome element of DNA which are found in the cytoplasm of a bacterium, as illustrated in Figure 2.[25] The process of conjugation
Abstract (½ pagina) This lab report dealt with the analyzation and transmission of genetic traits in monohybrid and dihybrid crosses using Caenorhabditis. Mutations will be either dominant or recessive or X-linked or autosomal. Where using a sterile pick you will pick certain worms and place them in a new petri dish for them to reproduce and observe new progenies, mutations and different crosses. should provide a summary of the entire lab report so that a reader could get the “gist”
removal of hydrogen from the OH group. After separation and purification, an Infrared Spectrum will be run to determine the composition of the recovered material. Infrared Spectroscopy is a very powerful technique used in the determination of molecular structure and the identification of unknown organic materials. The infrared spectrum yields direct information about the presence or absence of key functional groups. “The region of the infrared spectrum which is of greatest interest to organic
Genii’s In the Making “…molecular machines of nanotechnology will increase, by orders of magnitude, our individual and collective capacity to transform desires into material reality” (Crandall, viii). What is the goal of technology? Most answers include some form of ways of increasing speed, or decreasing size all for the ultimate goal of convenience. Ok, so what is convenience? Convenience is the skill level at which something can be achieved, in other words, how desires can be met
Biomedical Sensors- Advancing Medical and Biotechnology Introduction to Sensors Sensors are regarded as small, tiny and intelligent devices that are used to measure physical variable like Temperature, Humidity, Gas, Velocity, Flow Rate, pressure and many others. According to American National Standards Institute, “A Sensor is defined as device which provides a usable output in response to a specified measure.” A Sensor acquires a physical quantity and converts it into a signal suitable for processing
substances. Thus in the present study, the earthworm skin extracts were prepared and subjected to antimicrobial activity which was determined by the agar well diffusion method. The antimicrobial substances showed activity against Serratia marcescens. The molecular weight of the peptide from skin extract was found to be ~3kDa by SDS – PAGE and confirmed by zymogram analysis. Further the earthworm was identified by morphological and COI gene sequence analysis and resulted to be Wegeneriona sps. This study may
compounds across a cell’s plasma membrane. The cell must be able to transport these compounds across the membrane in order to regulate the characteristics of this transport (Reece, 124). The plasma membrane is selectively permeable because of the molecular composition of the plasma membrane, the cell is selective about what comes in and out. (Physio ex 9.1). Cell transport is separated into two distinct aspects: passive transport and active transport. Passive transport refers to the movement of molecules
1.1 What is the microbiome The human microbiome is the collective ensemble of a wide diversity and density of living micro organisms found both in and on the human body (i.e. the collective genome of the human microbiota). Its relevance has become so important as of late that is has taken its place at the top of 21st century scientific discoveries. (Ash 2014) It consists of mostly bacteria but also includes some archaea, fungi, viruses and protozoa. The main microbiome communities active in the body
Annotated Bibliography Ballard O., & Marrow, A. L. (2013). Human Milk Composition: Nutrients and Bioactive Factors. National Center for Biotechnology Information. Retrieved from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586783/ This article goes into depth on the different components that make up breast milk’s nutrient content. The article also goes over the different types of milks that a mother produces when feeding her infant. It also brings up that every mother’s milk is different because
HISTORY OF BIOTECHNOLOGY Suhail Muzaffar National Centre for Biological Sciences, GKVK Campus, Bellary Road Bangalore 560065, India Keywords: Biotechnology, Ancient Biotechnology, Classical Genetics, Discovery of DNA, Genetic engineering, Outline of the Chapter Sl. No. Contents 1 Overview 2 Biotechnology Time Lines 3 Periods of Biotechnology History 3.1. Ancient biotechnology 3.2. Classical biotechnology 3.3. Modern biotechnology 4 References 1. Overview The term “Biotechnology” was first
that our novel split-Cre complementation system introduces both temporal and special control of site specific recombination using Cre recombinase enzyme. This system solved many drawbacks have emerged during the extensive use of Cre recombinase in molecular biology. The complemented protein is almost as efficient as the Full CRE in the recombination activity (~95%). Moreover, each fragment lacks the recombinase activity. This system allows precise genetic manipulation. It has a special importance in
the development and introduction of molecular-based techniques into the labora... ... middle of paper ... ...nd Notomi, T. (2009) 'Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases.', J Infect Chemother, 15(2), pp. 62-69. Muldrew, K.L. (2009) 'Molecular diagnostics of infectious diseases',Curr Opin Pediatr, 21(1), pp. 102-111. Rastogi, G. and Sani, R.K. (2011) 'Chapter 2 :Molecular Techniques to Assess Microbial Community
polymerases, either in separate (‘two-enzyme/two-tube’) or in single (‘two-enzyme/one-tube’) reactions, as the use of dedicated enzymes with different proper... ... middle of paper ... ...rse transcription polymerase chain reaction assays." Journal of molecular endocrinology 25.2 (2000b): 169-193. (article given) 3. Huggett, J., et al. "Real-time RT-PCR normalisation; strategies and considerations." Genes and immunity 6.4 (2005a): 279-284. 4. Huggett, J., et al. "Real-time RT-PCR normalisation; strategies
Pesticides and Fertilizer run off from farms causes an overwhelming negative effects on the environment. Pesticides contaminate the water and poison fish both killing them and causing biological magnification in the fish that are higher up in the food chain. When humans eat these fish the risk of being harmed by pesticides is increased. Tobacco plants were engineered to express a gene that detoxifies 2,4-dichlorophenoxyacetate ( 2,4-D), one of the most commonly used pesticides world wide, and removes