Free Lipase Concentration Essays and Papers

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Free Lipase Concentration Essays and Papers

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    An Investigation into the Effect of Lipase Concentration on the Hydrolysis Of Fats Using the data loggers a recording of the pH was taken every 5 seconds and for each experiment the data loggers produced graphs of the change in pH. From each of these graphs a gradient was calculated which showed the rate of pH change per second. Firstly I calculated the gradients by choosing the steepest section of the graph and dividing the change in pH of this section by the time. However this method proved

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    The Tay-Sachs Disease

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    The discoveries of Warren Tay and Bernard Sachs in 1881 and 1887 laid the groundwork for our current understanding of the disease that has plagued the Jews for generations. Named after these foundational researches, Tay-Sachs disease is a lysosomal storage disorder (LSD) that causes severe mental degeneration in the affected individuals (Valsamis and Valsamis 1963). The nature of the disease’s inheritance and the history of Eastern European Jews in small crammed communities and their resistance to

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    Effect of Temperature on the Rate of a Lipase Reaction I am going to look at the effect of temperature on the rate of a Lipase Reaction, and to look at this I will be changing the temperature variable. In order to measure this, I will be doing the following practical: 1) Add 5cm³ of milk and 1cm³ of Lipase solution at different temperatures. 2) Then I will measure the reaction every 30 seconds, from 30 seconds to 2 minutes with a pH probe (as lipase breaks down into fatty acids) to test

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    The Effect of Temperature on an Enzyme's Ability to Break Down Fat Aim: To investigate the effect of temperature on an enzyme’s (lipase) ability to break down fat. Hypothesis: The graph below shows the rate increasing as the enzymes get closer to their optimum temperature (around 35 degrees Celsius) from room temperature. The enzyme particles are moving quicker because the temperature increases so more collisions and reactions occur between the enzymes and the substrate molecules

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    A Brief Note On Lipases

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    4.2 CASE STUDY: LIPASES 4.2.1 Background Lipases are a type of hydrolases which catalyze the conversion of fats/oils to free fatty acids and glycerol. Lipases are placed after proteases and carbohydrases in the world enzyme market and have about 5% of enzyme market. They are one of the most important group of biocatalysts for biological applications and are quite important enzymes from both physiological and biotechnological view point. The rising interest in lipases is mainly because of wide industrial

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    Lipase Synthesis

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    Lipase (E.C. 3.1.1.3) has shown its potential in catalyzing an esterification reaction (Rajendra et al., 2009; Sangeetha et al., 2011; Adlercreutz, 2013). The explorations of low-priced lipase sources have been carried out for industrial applications (Tuter et al., 2003; Haas et al., 2001; Abigor et al., 2002; Natarajan et al., 2010). One of lipase sources is rice bran, which can be used as a low-priced biocatalyst for an esterification reaction (Bharwaj et al., 2001; Chuang et al., 2010). However

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    phytotoxiicty of nanoparticels. Environ. Sci. Technol, 43(24), 9473-9479. doi: 10.1021/es901695c Titley-O'Neal, C. P., Macdonald, B. A., Pelletier, E., Saint-Louis, R., & Phillip, O. S. (2011). The relationship between imposex and tributyltin (tbt) concentration in strombus gigas from the british virgin islands.Bulletin of marine science, 87(3), 421-435. doi: http://dx.doi.org/10.5343/bms.2010.1093 Wang, F., Gao, F., Lan, M., Yuan, H., Huang, Y., & Liu, J. (2009). Oxidative stress contributes to silica

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    and the drug concentration in the filtrate was determined by high performance liquid chromatography (HPLC) analysis after appropriate dilution. The oil phase and surfactant that showed high solubility of HCA was used in the preparation of microemulsion. Construction of pseudo-ternary phase diagrams Water titration method was used in constructing pseudo-ternary phase diagrams at ambient temperature by choosing tween 80 as surfactant and ethanol as cosurfactant to obtain the concentration range of components

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    solution provides the optimum pH for the bacteria to work at. Following the bacterial action, the solution is drained off of the impermeable rock or pumped out of the ground through the remaining holes and the remaining solution prepared for concentration and extraction of the Cu2+ ions. 200 Extraction and stripping then occur, here the copper ions are selectively removed from the leaching solution by the "ligand exchange solvent extractionγ " method. During this a good ligand (a molecule

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    Tuber Cells

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     Specimen tubes with stoppers x6  1cm3 diameter cork borer  razor blade  filter papers  balance  distilled water  sucrose solutions with varying concentrations  potato cut into small discs Results. (Fig 1.0) Sucrose concentration (M)     Initial mass (kg)     Final mass (kg)     Change in mass (%) 0.10     0.95     1.25     31.57 0.20     0.94     1.13     20.21 0.30     1.03     1.15     11.65 0.40     0.95     1.05     10.52 0

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