Investigation on the Enzyme Trypsin An Investigation determining a factor affecting the rate of digestion of gelatin by the protease trypsin. Introduction An enzyme is a biological catalyst, which speeds up reactions. An example of this in the human body is trypsin (a protease produced in the pancreas and used in the stomach), which catalyses the digestion of gelatine, a protein. For this investigation, a photographic film will be the source of the gelatine. I will be able to identify
An investigation of the enzyme succinate dehydrogenase. Introduction Enzymes are a catalysts that speed up a chemical reaction inside of a cell without being consumed or changed by the reaction. (Wright, W. 2015) Enzymes catalyse reactions by lowering the activation energy that is required for the reaction to occur. (Nature, 2012) In this experiment we will be using Succinate dehydrogenase which is an enzyme that has been extracted from chicken hearts, succinate dehydrogenase is an enzyme of the
Assessment for DCP and CE Determining if temperature affects enzyme activity This investigation is using a range of temperature in order to find weather or not Temperature affects enzyme activity. I will: 1. Observe the effects of different temperatures on enzyme activity 2. Infer the relationship between the two 3. Use a line graph to display data and draw a conclusion Research Question How does temperature affect enzymes activity? Variables Independent Variable: Temperature
Investigation of How the Concentration of Catalase Enzyme Affects the Rate of Reaction Aim: To find out how the concentration of Catalase Enzyme will affect the enzyme activity and the rate of reaction towards Hydrogen Peroxide. (H O ) Prediction: I predict that with the higher concentration of enzyme, the likelihood of it breaking down molecules will be greater because there will be more enzymes to work at the substrate and the chances of it colliding will be higher making the activity
Breakdown of Starch Investigation: To find the effect of the concentration amylase on the rate of breakdown of starch. Prediction The lower the concentration of amylase, the slower the breakdown of starch will be. If you double the amount of amylase, the breakdown of starch will be quicker because there are twice as many active sites. I expect to see a graph like: [IMAGE] Rate of Breakdown [IMAGE] Concentration of amylase Research Enzymes are made of long protein
Rate of Reaction Using Enzymes In this bit of coursework I will be investigating the rate of reaction in which enzymes are the catalyst breaking down a substrate. The substrate I am going to use is Hydrogen Peroxide (H2O2, water with another Oxygen), this molecule usually breaks down on its own accord, but also the rate of decomposition can be increased with the use of a catalyst; in this case the catalyst is a biological one called an enzyme. Hydrogen Peroxide slowly breaks down on its
Investigating The Effect of Temperature on the Structure of an Enzyme Introduction: For my GCSE Biology assessment I will be investigating the enzyme amylase with the substrate starch. This reaction, which I am going to investigate, is called the protein test for starch. Aim: My intention for this observation is to examine how the enzyme catalyses are affected by changes in temperature. Safety Precautions: In this investigation I am going to make sure that everything is as safe as
Effect of temperature on the enzyme and the amount of foam produced Introduction The enzyme used in this investigation was catalase, catalase is a type of protein called enzyme and the function of an enzyme is to catalyse(speed up) biochemical reaction. Catalysts such as enzymes are important in biochemical reaction, without them the reaction would occur too slow for an organism to survive. Catalase is present in most organisms which are in contact with oxygen, as it protects the organism from
to break down fat. Hypothesis: The graph below shows the rate increasing as the enzymes get closer to their optimum temperature (around 35 degrees Celsius) from room temperature. The enzyme particles are moving quicker because the temperature increases so more collisions and reactions occur between the enzymes and the substrate molecules. After this the graph shows the rate decreasing as the enzymes are past their optimum temperature (higher than). They are getting exposed to temperatures
either as single polypeptide or can be grouped together into multienzyme complex known as cellulosome. Cellulase system is composed of three main classes based on their activity toward a wide range of substrates. This is rather difficult, since the enzymes have overlapping specificities toward substrates which themselves are poorly defined. The three main classes are: a) Endoglucanases (endo-1, 4- β- glucanases or 1, 4- β-D-glucan 4-glucanohydrolases EC 3.2.1.4) hydrolyze cellulose chains at random
Factors that Affect the Rate of Respiration in Yeast Introduction ============ Yeast is one of the living cells, which can respire aerobically, and anaerobically in this investigation I am just taking into consideration respiration as whole. The equation of respiration in yeast is: Equation: There are different variables in this experiment. These consist of the range in temperature, concentration of glucose, amount of hydrogen carbonate solution used and amount of water used
Affects the Rate of the Starch Concentration In this piece of coursework, I have to carry out an investigation to find out how amylase concentration affects the rate of starch. Enzymes are biological catalysts that speed up the chemical reaction that goes on inside living things. An enzyme acts on substrate and may do its job inside or outside the cells. However, the rate at which enzymes work are affected by the following factors/variables: Concentration: In a more concentrated solution
What pH the Enzyme Named Peroxidase Works Best At The aim of my investigation is to find out what pH the enzyme named peroxidase works best at. We can find the enzyme peroxidase if we grind up celery, or most other animal and plants. A chemical reaction in plants and animals produce hydrogen peroxide, this can poison them if it is allowed to accumulate. The enzyme peroxidase acts as a catalyst for decomposing hydrogen peroxide, it is broken down into water and oxygen. The reaction is:
Investigation into the Digestion of Milk by Trypsin Background Knowledge ==================== To investigate the effect of trypsin on milk a number of separate experiments will be performed whereby milk is digested by trypsin under controlled conditions. Specific variables will be changed by calculated amounts to gauge their individual effects on the rate and amount of reaction that occurs. Trypsin is a biological catalyst, (a substance that speed up a
increase, but will then stop rising. When yeast respires anaerobically, bu... ... middle of paper ... ...d. I hav creatd another experiment which investigates the best temperatures at which yeast enzymes work best at. I want to investigate further into what exactly is the yeast enzymes' optimum temperature, as it was 101cm3 at 50C, but as I mentioned before - the dough was at unevern temperatures, as it was hard to control it without the special abilities of laboratory machines. I know
The Effects of Temperature on the Action of Enzymes Aim: To see how changing the temperature affects the action of the enzyme. ------------------------------------------------------------------ MY HYPOTHESIS: I think that as the temperature increase the quicker the reaction will become ------------------------------------------------------------------- Outline of method You add ice to 150cm³ of cold water in a beaker to bring its temperature down to 10cº. Then a fixed volume of
Investigation to Determine the Relationship Between Surface Area and Rate of Oxygen Production To determine the relationship between surface area and rate of oxygen production when a potato is placed in hydrogen peroxide (H2O2) solution. Background Information ---------------------- Potato contains enzymes such as catalase. Catalase are protein molecules, which are found in animal and plant cells. Catalase speeds up specific reactions in the cells. They all have very specific duty
immunoglobulin-binding proteins, enzymes, lectins, carbohydrates, avidin/biotin system and antibodies are used as ligands (Hage, 2006). Immunoglobulin-binding proteins, namely protein A which is produced by Staphylococcus aureus and protein G which is produced by streptococci, are the ligands that are used in the vast majority of bioaffinity chromatographic applications (Tetala and van Beek, 2010). However, enzymes and enzyme inhibitors can also be used as affinity ligands (Hage, 2006). Immobilized enzymes are widely
Aim: To investigate the effect of pH of hydrogen peroxide solution on enzyme activity of the catalase enzyme (yeast) and the O2 froth produced (ml) after 1 minute. Hypothesis: That an increase of hydrochloric acid to a solution where yeast cells are exposed will increase the rate amount of enzyme catalase (O2 froth) produced, as from my research it has been proven that with some enzymes the optimum pH is around 2 (stomach enzymes). Variables: Independent Variable: pH The independent variable being
Any difference that is observed is entirely due to chance and not because of the independent variable. Alternate Hypothesis: Increasing concentration of the enzyme, pectinase, will increase the volume of juice produced and the clarity. The reason behind this hypothesis is the fact that at a higher concentration of pectinase, more enzyme molecules are present to break down the pectin in the cell wall of the apple and since pectins have a high affinity towards water and form hydrophilic groups, a