Biology: Separation of Proteins Lab Report 1: Separation of Proteins Abstract/Summary: “Proteins account for more than 50% of the dry weight of most cells, and they are instrumental in almost everything organisms do” (Campbell, 1999). The significance of proteins to the continuation of our biological systems is undeniable, and a study of how to quantify proteins seems an appropriate introduction to our studies of biology. In order to study proteins we must first know how to separate then quantify
Figure 1 - Ponceau Stain blot Key : 1) Protein Molecular Weight Marker 2) Recombinant pET41(+) EGFP plasmid (positive control) 3) Non-recombinant pet41(+) EGFP plasmid (negative control) 4) Green colored clone properly expresses EGFP (green) 5) White clone that does not express EGFP (red) 6) Unknown sample (blue) 7) Purified GST-EGFP control A Ponceau stain can bind and identify all proteins. Lanes 2, 3, and 4 (our recombinant, nonrecombinant and green colony, respectively) have a slightly
purification of a protein. Specifically, the purification of the enzyme acid phosphatase from wheat germ. Through three major steps we were able to perform this purification. The steps involved were disrupting the source cell, selectively purifying the enzyme from contamination, and finally preserving the original structure of the enzyme and preventing degradation. Aliquots, or small volumes, of the solution were collected from each step in the purification process for enzyme activity and protein content.
Lab W 7-9:30 Protein Concentration; Utilizing Spectrophotometer to analyze commercially available food products to Justify FDA food Labels Scott Zoebisch 10-14-2015 Abstract Proteins help shape the physical body and the world. The consumer relies on proper FDA labeling of protein concentration. This Lab report will discuss the importance of proteins, proper food labeling and how the protein concentration is analyzed. The experimental use of the Bradford Protein Assay and Spectrophotometer
Protein extractions from unidentified fish samples were separated according to the molecular weights by SDS polyacrylamide gel electrophoresis. Since some of these proteins are shared between fishes, phylogenetic evaluation was reached. Western blot analysis was used to identify four unknown species of aquatic animals via comparison of actin/myosin bands. According to the results of this assay, the best estimate is that the unidentified aquatic animals are specimens of salmon, tilapia, cod, and shrimp
a mild skin reaction to deathly anaphylactic shock. Although peanuts cause allergies, they are often used in food processing. They provide important nutrients such as fats and proteins. While there are other foods that can provide similar nutrients (i.e. soybeans, beans, etc.), peanuts are still commonly used. The proteins found in peanuts are the cause of peanut allergies. There are a total of thirteen different
Chloroplast fractionation: Nucleic acid and protein analysis via gel electrophoresis ABSTRACT: Chloroplasts carry out photosynthetic processes to meet the metabolic demands of plant cells (Alberts, 2008). They consist of an inner thylakoid membrane and a stroma. (Parent et. al, 2008).In this experiment we demonstrate the unique protein compositions of isolated thylakoid and stromal fractions from broken and whole spinach chloroplasts. Because these compartments carry out different metabolic processes
It was a cold winter’s night when Abraham decided to get up for a walk. He sat in bed, threw his feet on the floor and rose like he had done countless times before with his cane in his hand, he put one foot in front of the other and followed with a turn but suddenly he heard a loud snap. He immediately found himself on the floor in agonizing pain. Later it was revealed that Abraham had broken his femur onset by Stage 3 bone cancer. After two long years of rehab and chemotherapy, Abraham lost his
University. 29 Oct 2006. HKA_Documentation.htm> Renart J, Reiser J, Stark GR. "Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure", Proc Natl Acad Sci U S A, 1979 Jul;76(7):3116-20. “Selective sweep”. Wikipedia. 2006. 13 November 2006. Towbin H, Staehelin T, Gordon J. "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications" Proc