Amylase Activity Essays

  • The Effects of Enzyme Concentration on the Activity of Amylase

    986 Words  | 2 Pages

    The Effects of Enzyme Concentration on the Activity of Amylase To investigate the effect of Amylase concentration on its activity. the relative activity of Amylase is found by noting the time taken for the starch substrate to be broken down, that is, when it is no longer gives a blue-black colour when tested with iodine solution. This time is referred to as the achromatic point. Equipment: v Amylase solution 0.1% v Starch Solution 1.0% v Distilled water v Iodine in potassium

  • Investigating the Effect of Copper Sulphate on Amylase Activity

    3341 Words  | 7 Pages

    Investigating the Effect of Copper Sulphate on Amylase Activity Aim The aim of my experiment is to observe the affect on amylase when adding copper sulphate to a starch solution. Introduction Enzymes are that act as catalysts, in other words they increase the rate of chemical reactions. Consider the following general reaction between two substances, A and B, which react together to form a product, substance C: A + B = C In biological systems, this reaction might occur very

  • The Effect of pH on the Activity of the Enzyme Amylase

    1088 Words  | 3 Pages

    The Effect of pH on the Activity of the Enzyme Amylase Aim :- To find the effect of pH on the activity of the enzyme amylase. An enzyme is a type of protein found in all living cells. Enzymes act as biological catalysts, breaking down substrates without needing a high temperature, allowing all the chemical reactions of metabolism to take place, regulating the speed at which they progress, lowering the activation energy and providing a means of controlling individual biochemical pathways

  • Temperature And Ph Effect On The Enzymatic Activity Of A Amylase

    1153 Words  | 3 Pages

    Temperature and pH effects on the enzymatic activity of a-amylase Introduction Enzymes are fundamental molecules within Organisms. They are the biological catalysts of the cells; enhancing the rate of biochemical reactions, ensuring metabolic needs are met. These molecules are identified as globular proteins with a three dimensional structure and are composed of one or more polypeptide chains. The polypeptide chain or chains within an enzyme are folded to form a specific active site. Each different

  • A Biological Catalyst

    570 Words  | 2 Pages

    A Biological Catalyst A Biological catalyst is a catalyst that is produced organically. In other words, a cell makes it. It is usually a protein or steroid molecule that works to catalyse a specific reaction. For example, amylase is a biological catalyst. Biological catalysts are called enzymes. Reactions take energy to get them started. This energy is called the activation energy. Enzymes catalyse reactions inside organisms. A catalyst is a molecule that acts

  • Amylase Lab Report

    1131 Words  | 3 Pages

    temperature on amylase converting starch to maltose. Lab Group 3 Abstract This experiment shows the effect of rising temperature on enzyme amylase activity on converting starch to maltose. The reason for conducting the experiment is to find optimal temperature for enzyme activity. The enzymes that will be comparing are fungal and bacterial amylases. The enzymes were set at 4 temperatures 0-850 Celsius and checked at 4 different times from 0-10. The results showed the enzymes activity increased until

  • Essay On Starch

    1286 Words  | 3 Pages

    starts in oral cavity and involves the enzyme amylase, it is produced by the salivary gland, and it is able to hydrolyze the alpha -1,4 glyosidic linkages in starch. (Tracey 2016) The presence of salivary amylase makes the process of digesting starch to occur in a much faster and efficient manner, since it does not have to wait till the food mixture to get into small intestine to start the breakdown of starch. The variation of this enzymatic activities among individuals are found to be significant

  • Analysis Of The Digestion Of Carbohydrates By Salivary Amylase

    2634 Words  | 6 Pages

    experiment “Digestion of Carbohydrates by Salivary Amylase” this dealt with a test where amylase was observed from a saliva sample and starch mix. Salivary amylase is a digestive enzyme that catalyzes the hydrolysis of starch of starch into simpler compounds (Saunders 1). The main function for salivary amylase is to break down starch. When it comes to starches, they are known to be complex carbohydrates. In order to see whether or not the amylase broke down the starch, you can add iodine to the saliva

  • Following the Progress of an Enzyme Controlled Reaction

    3587 Words  | 8 Pages

    enzymes for this by not actually interfering with the reaction its self but basically align the two substrates on the active site of the enzyme. Amylases are widely spread enzymes that hydrolyse starch to maltose. They are often found in two forms, a-amylase, which degrades starch molecules into, fragments 10 glucose residues long and b-amylase, which breaks down these into maltose, made up of two glucose molecules. Both work by hydrolysis adding one molecule of water across glycosidic link

  • Affect of the Rate of Reaction of Amylase on Starch and How Its Affected by the Concentration of the Substrate

    993 Words  | 2 Pages

    Affect of the Rate of Reaction of Amylase on Starch and How Its Affected by the Concentration of the Substrate Equipment list Ø Test tubes Ø Measuring cylinder Ø Thermometer Ø 3 pipettes Ø Starch solution Ø Amylase Ø Iodine solution Ø Water Ø Water bath Ø Spotting tile Ø A stop watch Ø Goggles Ø Beakers Health and safety ================= Remove all hazardous jewellery such as dangling earrings, bracelets, and chains. Make sure items of clothing

  • The Effects Of Emulsification On The Digestion Of Lipids

    1266 Words  | 3 Pages

    In part A of the lab, our group measured the effects of emulsification on the digestion of lipids in the presence of cholic acid, a purified bile salt, and distilled water. The tube containing vegetable oil and no bile began to separate into two layers within the first minute of being mixed together. Although there were no clear distinctions within the first 15 seconds, by the fifth minute, there appeared two separate layers; one resulted in a yellow appearance while the other one was clear. As

  • How Does Optimum Temperature Affect The Activity Of Alpha Amylase

    501 Words  | 2 Pages

    Temperature and pH have large effects on the enzymatic activity of alpha amylase. This was determined by measuring the reaction rates by varying the temperatures and pH of a reaction. Figure 1 indicates that as the temperature increases the reaction rate increases. When the reaction rate reaches its maximum level this indicates the optimum temperature. The optimum temperature is where the enzyme preforms at its highest capacity. The optimum temperature in relation to this experiment was 37°C. This

  • Amylase Lab Report

    604 Words  | 2 Pages

    When the enzyme amylase comes in contact with the starch molecules, it decreases the energy needed for the chemical reaction that breaks starch down into maltose, a simple sugar. The maltose molecules, or substrate. Fit perfectly into the enzyme’s active site, and thus the activation energy is reduced and the reaction can take place. This won’t work, however, if the enzyme is denatured. Denaturing occurs when either the temperature or pH of the enzyme is changed. In our lab, denaturing was simulated

  • How Amylase Concentration Affects the Rate of the Starch Concentration

    1207 Words  | 3 Pages

    How Amylase Concentration Affects the Rate of the Starch Concentration In this piece of coursework, I have to carry out an investigation to find out how amylase concentration affects the rate of starch. Enzymes are biological catalysts that speed up the chemical reaction that goes on inside living things. An enzyme acts on substrate and may do its job inside or outside the cells. However, the rate at which enzymes work are affected by the following factors/variables: Concentration:

  • Investigating the Rate of Reaction between Amylase and Starch

    1086 Words  | 3 Pages

    Investigating the Rate of Reaction between Amylase and Starch Plan Aim: The aim of this investigation is to find out whether the volume of amylase affects the rate of reaction between amylase and starch. Prediction: I predict that the greater the volume of amylase then the faster the rate of reaction between the starch and amylase will be. I predict this because of the lock and key hypothesis. The lock and key hypothesis explains how the substrate molecule (the starch) fits inside

  • Role Of Rennin

    1305 Words  | 3 Pages

    Rennin – A Proteolytic Enzyme Major Discussion:  Role of rennin in helping young mammals digest milk  Role of rennin as an industrial catalyst to make cheese Proteolytic Enzyme: Any group enzymes that catalyze the hydrolytic degradation of proteins or polypeptides to smaller amino acids polymer is called proteolytic enzyme. Rennin is a proteolytic enzyme made by chief cells in the stomach. Rennin also known as “Chymosin”. Rennin enzyme plays an important role in helping young mammal digest milk

  • Amylase Lab Report

    1587 Words  | 4 Pages

    enzyme amylase from digestion system, which it has a big part in breaking down carbs into maltose, glucose and others. And our data by end of the lab could lead us to the specific conditions which are required for amylase in order to do its job perfectly. Hypothesis: Carbohydrates: Starch + Amylase: amylase duty is to digest starch by catalyzing hydrolysis, which lead the starch to form into Maltose, which is around the same size as two joined glucose molecule, so base on this If we mix amylase by

  • Enzyme Temperature Lab Report

    616 Words  | 2 Pages

    Investigation to Determine the Effect of Temperature on the Activity of the Enzyme Amylase I am trying to find out if changing temperatures affects the activity of enzymes. The Input variable I will test is temperature; the range I will use for this is 0-80°C. The out come variable to be measured is the speed of the reaction. Hypothesis I believe that at a higher temperature the enzyme will react quicker, but at a certain temperature the enzyme it will stop working

  • Investigating Amylase

    1938 Words  | 4 Pages

    Investigating Amylase Aim: The aim of the experiment is to investigate how effectively the enzyme amylase breaks down starch at different temperatures, and therefore to find the optimum temperature that amylase digests starch. Introduction: Thousands of chemical reactions take place in our cells and those reactions need to happen quickly in order to keep us active. These chemicals are called ENZYMES. Enzymes make reactions happen at a much faster rate. Enzymes come in two main

  • Investigating How Quickly Amylase Breaks Down Starch With Varying Temperatures

    607 Words  | 2 Pages

    Investigating How Quickly Amylase Breaks Down Starch With Varying Temperatures Instructions List I gathered the equipment shown below. I put 3 drops of iodine in each chamber of the spotting tile. I boiled the kettle and mixed together water from the kettle with water from the tap so the water reaches the correct temperature. The water was put into a beaker. 5ml of starch was put into a test tube. The test tube was placed into the beaker. When the water in the beaker was at the required