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    Isolation Of Bacteria

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    condition to the other for growth. These isolation techniques were developed by Robert Koch in 1883 in which he added agar to the liquid nutrient broth. With the addition of agar the liquid media was transformed into the solid media and helped in isolating different bacterial colonies. In this media the nutrient broth supplemented the nutrientinal requirements of the bacteria whereas agar provides the solid substrate for bacterial growth. The pure culture contains a similar kind of bacteria that has risen

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    sample was collected from the extracted tooth using an excavator under aseptic conditions. The clinical samples were mixed well using a magnetic stirrer before incubation. The samples were then inoculated using the streak plate technique on to nutrient and sabouraud dextrose agar under various culture conditions- aerobic, microaerophilic, and anaerobic culture conditions for each patient sample (Holding and Colee, 1971). The organism isolated was identified on the basis of morphological, cultural

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    Following several serial dilutions, individual yeast colonies were able to be produced on a agar medium. Through the technique of replica-plating, cells from these colonies were able to be transferred to three separate petri plates in the same spatial arrangement they were found on the original plate. One of these plates was prepared with a standard agar medium, while the other two contained a copper-based agar. The cultures were consequently refrigerated in order to prevent growth after the yeast

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    Bacterial Growth Essay

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    Every organism requires a specific environment in order to survive. Bacteria alike, different types of bacteria are able to survive and reproduce in different types of environment. Some factors that affect the growth of bacteria include temperature, presence of certain gases and pH of the medium it is in. In this experiment, the variable that was changed was temperature. Temperature is one significant factor that affects the growth of bacteria. Each bacterial culture has its own minimal, maximal

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    Pbluescript Plasmid

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    the patched colony, indicating that haemolysis of the blood agar occurred. Conversely, non-haemolytic colonies were classified by a lack of a white ring, which indicated that no haemolysis took place. While the previous experiment identified colonies containing recombinant DNA, the patching experiment distinguished which colonies contained the hlyA fragment and which ones did not. Colonies that could cause haemolysis of the blood agar plate indicated that recombinant DNA taken up contained the hlyA

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    Bacterial Species

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    Section I- Introduction and definition of a Bacterial Species The field of biology has been a challenge for over four hundred years. An essential part of this problem would be the field of Microbiology. In Microbiology, there is application in which we group organism into different species. This application is called taxonomy. Taxonomy is the practice and science of classification of different organism in the same domain. With taxonomy, we see a short glance on how species are categorized but what

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    Endospore Test Essay

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    that the tube contained two cultures. With that being said, to be able to identify the two cultures separately, the quadrant streak method was applied (Brown, 2007). The quadrant streak method involves a petri plate, loop, Bunsen burner and the skill of being able to properly streak the plate so the cultures can separate and become pure. In order for the performance of the quadrant streak method B to be successful, the skill of proper aseptic technique was applied (Brown, 2007). The aseptic technique

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    gibberellin in a barley seed will increase the amount of starch broken down surrounding the seed in the agar plate. This will be seen as an increase in the clear area around the barley seed on the agar once iodine is added. To carry out the experiment starch-agar Petri dishes are needed. Gloves must be worn to prevent contamination of the Petri dishes and so not to hinder the results. To make the starch-agar: (These figures make about 12 Petri dishes)3 1) Measure out 200cm3 of water using

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    P20 Unit 2 Lab Report

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    For this experiment, each group had to gather five microfuge tubes and a foam rack for them to be placed in. Each of these tubes were labeled 1 through 5 using a wax pencil. Then each group gathered a test tube containing 5 ml of Luria Broth, a P20 micropipette, a P200 micropipette, and a P1,000 micropipette. Then one person in each group is going to be the designated mircopipetter. This person will pipette 998 l into the first microfuge tube, 995 l into the second microfuge tube, and 900 l into

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    Common Microorganisms

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    organisms can easily be seen using differing types of agar, which creates an ideal environment for the organisms to form colonies, which are groups of hundreds of organisms that can be seen with the naked eye. In order to see individual microorganisms, it is necessary to use the magnification of a high-powered microscope. These techniques of microbiology are used in the following five experiments. The first experiment used Trypticase Soy Nutrient Agar (TSA), which can grow a wide variety of organisms

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    Nalidixic acid(30mcg), Norflexin(10mcg), Roxythromycin (30mcg)and Streptomycin(10mcg). The essential oils were Eucalyptus oil, Lemongrass oil, Windergreen oil and Mint oil. COMPOSITION OF MUELLER-HINTON AGAR Beef infusion from : 300.0g Acid hydrolysate of casein : 17.5g Agar : 17.0g Starch : 1.5g The pH was adjusted to 7.3 ±0.2 at 25°C using 0.1 N hydrochloric acid (HCl) or 0.1 N sodium hydroxide (NaOH) solution

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    Mutualism Essay

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    bacteria will be grown in different agar plats. Different agar plates are used to grow specific

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    m The purpose of the exercise was to determine the carriage rate of staphylococcus aureus in the nasal carriage of students microbiology I students at RMIT university in 2016 and to compare with similar studies in 2012- 15 and 2 published studies from a similar demographic introduction Staphylococcus aureus is an important pathogen because of its mutations in the 1960s which lead to the developed of a strain known as Methicillin-resistant Staphylococcus aureus (MRSA) (Schinasi et al., 2013) which

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    Cotton Swab Lab Report

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    preform this experiment you will need; cotton swabs, agar plates, microscope, unused slides, oil immersion, nigrosin, and crystal violet. The first task we must do is use the cotton swabs and swab an item out side of the laboratory, that has the capability of containing either yeast, bacteria, and mold. My lab partners and I chose to swab one of our group members cell phone. Once we swabbed the phone with the cotton swab, we then each had a plate of agar. To start the process of the transfer of the microorganisms

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    unknown bacteria

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    surface in a seven day time span in a very active household. A sample was obtained from the television button and was swabbed on the nutrient agar plate. There was a variety of testing performed on the sample that was obtained to help to identify what kind of bacteria there might be. The bacteria were first transferred from the nutrient agar plate to another plate using aseptic technique. To begin the testing of the bacteria, a gram stain test was performed to determine if the bacteria was gram positive

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    Acne is described as the most common skin disorder that affects 85% of individuals ranging from pre-teenagers to grown adults, specifically between the ages of 12-24 (Tahir 2010). In the midst of the 50 million people affected with acne vulgaris, adolescences are generally most susceptible due to elevated sexual hormones (Zaenglein et al., 2016). Numerous factors increase the production of acne vulgaris. Inadequate diet, menstruation, sweating, over exposure to ultra violet rays and excessive stress

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    multi

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    against Staphylococcus sp., Bacillus sp., Klebsiella sp., Pseudomonas sp. and Proteus sp. Nutrient agar plates were prepared by pouring 15 ml of media into sterile Petri dishes. The agar plates were allowed to solidify for 5 minutes and 0.1% inoculum was swabbed uniformly and allowed to dry for 5 minutes. The finished fabric with the diameter of 2.0 ± 0.1 cm was placed on the surface of medium and the plates were kept for incubation at 37 ºC for 24 hours. At the end of incubation, the zone of inhibition

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    Background Info: Mold is a group of fungus that are a decomposer in nature. They are basically a single celled organism with thousands of nuclei. They nearly have the same life cycle as fungi. They are made up of filaments, called hyphae.They can be found in shady, damp areas outdoors, like rotting logs, or any place with decomposing vegetation. There are also parasitic molds, that live off a host. . Mold can reproduce with itself, asexually, and with other molds, which would be sexual reproduction

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    ribotale

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    cell) and spectinomycin resistance selection marker, from chromosomally integrated gene cassette, and wild type araC (Lutz & Bujard, 1997). MG1655Z1 was maintained at 4°C on LB agar plate, supplemented with spectinomycin. DH10B cells were used for the routine cloning. Strains harboring plasmids were maintained on LB agar plate supplemented with required antibiotic(s). When required, antibiotics chloramphenicol (50 µg/ml), carbenicillin (50 µg/ml) and ampicillin (50 µg/ml) were supplemented in media

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    Mapping the Genes of Sordaria Fimicola

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    The main purpose of this experiment is to examine the results of wild-type mutant crosses which influence the arrangements of ascospores in asci in the fungus Sordaria fimicola. These resulting arrangements help calculate the map distance between the centromere and spore color genes in Sordaria. My hypothesis was that due to so many group observations accounted in, the data will be underestimated and the results will not fit into the chi square table. A sample from Petri dish with both mutant stock

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