Essay on The Insertion of L. hesperus TuSp1 gene into B. mori

Essay on The Insertion of L. hesperus TuSp1 gene into B. mori

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Figure 1. TuSp1 molecular structure (Solution structures of TuSp1 domains).
The amino acid sequence of the TuSp1 gene contains a signal peptide at the N terminus that is adjacent to the NTD non repetitive sequence. There is also a non repetitive sequence at the C terminus (CTD). Linked to the CTD there is a RP2 type two repetitive sequence. Also present are 20 identical type 1 repetitive sequences ( RP1). Pictures A-H represent the different TuSp1 gene domains. Pictures A-D represent NTD, RP1, RP2 and CTD domains respectively. Pictures E-F represent and the hydrophobic and charged surfaces of NTD, RP1, RP2 and CTD respectively. The color code is blue for positive charge, yellow for hydrophobic, red for negative charge, and white for a neutral surface. The hydrophobic patches are circled in black. Micelle like structures, essential intermediates in silk formation, are formed by more hydrophilic terminal domains that are covalently linked with one or more repetitive domains. The less hydrophilic domains dictate the actual assembly of the protein. The structural transition and fiber formations mechanisms have not been found over a multitude of studies; thus, indicating more research on this topic is needed (Lin et al. 2009).

The objective of our experiment is to create a stronger, more durable and elastic silk product by genetically modifying silkworms (Bombyx mori) to express the egg case silk of the Western Black Widow spider (Latrodectus hesperus). Eggs from B. mori will be extracted, fertilized and the original silkworm silk protein, P25, will be silenced via CRISPR/Cas9. The TuSp1 gene will be extracted from L. hesperus, ligated with the promoter for the P25 gene and then inserted into a fertilized B. mori zygote via microi...

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Takemura, Y., Kanda, T., & Horie, Y. (2000). Artificial insemination using cryopreserved sperm in the silkworm, Bombyx mori. Journal of Insect Physiology, 46(4), 491–497. doi:10.1016/S0022-1910(99)00135-3

Tan, A., Fu, G., Jin, L., Guo, Q., Li, Z., Niu, B., … Huang, Y. (2013). Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori. Proceedings of the National Academy of Sciences of the United States of America, 110(17), 6766–70. doi:10.1073/pnas.1221700110

Thomas, J.-L., Da Rocha, M., Besse, A., Mauchamp, B., & Chavancy, G. (2002). 3×P3-EGFP marker facilitates screening for transgenic silkworm Bombyx mori L. from the embryonic stage onwards. Insect Biochemistry and Molecular Biology, 32(3), 247–253. doi:10.1016/S0965-1748(01)00150-3

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