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Clinical case of spinal cord injuries
Clinical case of spinal cord injuries
Urinary tract infection case study
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Summary of patient condition My patient name is Francis A. Johnson. She is a 52-year-old woman, who is under my care at a nursing home currently. She has been diagnosed with a urinary infection and this test was conducted from a urine sample. It is also documented in her medical file that she’s allergic to shell fish and iodine. Mrs. Johnson suffered a spinal cord injury 10 years ago. She has lost proper function in her hip and both legs muscles, including bladder and bowel as the result of an injury to the sacral nerves; previous to the infection. She didn’t say how the injury occurred, however this kind of injury can be a result of motor vehicle accident, falls, trauma, birth defects, degeneration and osteoporosis. Johnson requires an intermittent
The bacteria tested positive for being a facultative anaerobe, because the entire tube appeared cloudy. The bacteria grown everywhere in the test tube, meaning that my unknown bacteria can produce ATP in the presence of oxygen and it can also switch to fermentation in the absence of oxygen. A negative result would show a stub line after incubation, because the bacteria did not move. They can only move if they have flagella. A feather out or cloudy appearance of the test tube identifies a positive mobility result. After conducting the gram stain test, we still needed to confirm that the bacterium was truly a gram negative or gram-positive bacterium. The MacConkey media was used to grow gram-negative bacteria and inhibit the growth of gram- positive. The bile salts and crystalviolet ingredients are selective against gram-positive bacteria; it can also be used to differentiate between gram-negative bacteria. The MacConkey media allowed Providencia stuartii to grow in the test plate. I also did a lawn on the MSA plate, but the bacteria did not grow because MSA is selective against gram-negative. There is 5% salt in MSA limited the growth of gram- negative and Coliforms, such as E-coli. The second test that was done is the oxidase. This test was done to detect whether or not the organism is aerobic or not. To perform this test, I took a sterile
Taking a loop full of the bacteria and transferring it into the test broth was how the test was done. It was incubated at human body temperature. My bacteria result was negative because it did not change color from red to yellow. A positive-test shows a color change from red to yellow, because the bacteria ferment the sugar into acid, changing the color to yellow and the pH of acidic (pH 6-1). So I started up with an alkaline solution that was not metabolically changed by the bacteria into an acid solution. Gas can also be produced as a positive result. Another test that was done was the triple sugar iron test. A loop full of the bacteria was transferred from the TSA plate to the broth; a spiral streak was carefully done to prevent poking the broth. A positive result changes to a dark color and a negative test did not. This result shows that my bacteria do not ferment the sugars glucose, lactose and sucrose. Bacteria that can ferment these three sugars have the ability to produce hydrogen sulfide gas. The Gelatin test was to determine whether or not the organism has the enzyme gelatinase that hydrolyzes gelatin. A gelatin broth was obtained and was stabbed with the unknown bacteria. The media remained solid, meaning that the gelatinase enzyme was not present. Bacteria with this enzyme break down gelatin, making the solution liquefy. The last metabolic test that was done is the citrate. The test result was positive, the citrate Simmon tube
The isolate possesses some enzymes required for hydrolytic reactions. Hydrolytic enzymes found to be secreted from the bacterium, are -amylase, casein, and PYRase. In the starch hydrolysis and casein tests, there was a zone of clearing around the bacterium, which was indicative of the secreted enzymes necessary to break down starch and casein. In the PYR test, the presence of PYRase was detected by a color change to red on the PYR disc after the addition of the PYR reagent (p-dimethylaminocinnamaldehyde). Hydrolytic enzymes for which the EI tested negative were urease, gelatinase, and DNAse. In the Urea Hydrolysis test, it was observed that the urea broth did not have a color change, indicating that there was no urease secreted to break down urea in the broth. Similarly, there was no gelatinase present to break down gelatin in the Gelatin Hydrolysis test, so the nutrient gelatin remained solid. It was concluded that the EI does not possess DNase because there was no clearing zone around the bacteria, indicating that DNA had not been
One bacterium was gram negative. It underwent four different tests. These tests were the EMB test (Eosin Mehylene Blue), the Sulfur Indole Motility (SIM) test, the Urease test, and the Simmon’s Citrate Utilization test. The EMB test checks for a bacteria’s ability to ferment lactose. This test is accomplished by placing the bacteria on Eosin Methylene Blue agar. The agar is selective for gram negative bacteria and those bacteria that can ferment lactose will have colored growth, usually a metallic green sheen.
Streak plate technique was used to isolate pure culture for each bacteria (2). The Gram stain was used to determine Gram reaction and morphology of each bacteria (2) Selective and differential media such as, salt agar, MacConkey agar and blood agar were used for bacterial identification (2). Gelatin deeps were inoculated to detect production of gelatinase (2). Starch Agar plate were inoculated to detect amylase (2). Ocular reticle used to determine bacteria size (2). Motility deeps were inoculated to detect motility on bacteria (2). Thioglycollate broth used to determine oxygen requirements (2). Carbohydrate fermentation
They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species.
After the end of the experiment the unknown 10 sample was Staphylococcus epidermidis. Came to this conclusion by first beginning with a Gram Stain test. By doing this test it would be easier to determine which route to take on the man made flow chart. Gram positive and gram negative bacteria have a set of different tests to help determine the unknown bacterium. Based on the different tests that were conducted in lab during the semester it was determined that the blood agar, MSA, and catalase test are used for gram positive bacteria while Macconkey, EMB, TSI, and citrate tests are used for gram negative bacteria. The results of the gram stain test were cocci and purple. This indicated that the unknown bacteria were gram positive. The gram stain test eliminated Escherichia coli, Klebsiella pneumonia, Salmonella enterica, and Yersinia enterocolitica as choices because these bacteria are gram negative. Next a Blood Agar plate was used because in order to do a MSA or a Catalase test there needs to be a colony of the bacteria. The result of the Blood Agar plate was nonhemolytic. This indicated that there was no lysis of red blood cells. By looking at the plate there was no change in the medium. Next an MSA test was done and the results showed that there was growth but no color change. This illustrates that the unkown bacteria could tolerate high salt concentration but not ferment mannitol. The MSA plate eliminated Streptococcus pneumonia and Streptococcus pyogenes as choices since the bacteria can’t grow in high salt concentration. Staphylococcus aureus could be eliminated because not only did the unknown bacteria grow but also it didn’t change color to yellow. Lastly a Catalase test was done by taking a colony from the Blood Agar plate...
I also inoculated a tryptic soy broth (TSB), a nutrient gelatin deep, a motility agar deep, a fluid thioglycollate medium (FTM) tube, and a TSA plate with my unknown culture. All of these inoculated media were incubated until the next class period (about 48 hours). Then when I came to class most of my inoculated tubes and my streak plate appeared to have growth. The next step I took was making a gram stain to determine the gram reaction and cellular morphology of my unknown. I used my working slant to do this, after careful examination of the gram stain, I learned that my unknown was a gram-positive bacterium. I then preceded by making a negative stain to see the size of the cells of my unknown bacteria. The cell shape was cocci and the cells occurred in clusters of tetrads. After discovering that my unknown bacteria was gram-positive cocci, I turned to page 207 of the lab manual to narrow down my options, there was only four out of the gram-positive list that were
I began my test to classify my unknown bacteria by performing the Gram staining because according to the first period procedure of the laboratory manual and the Appending H, it was the first test that should be done to plan and proceed to the next tests. Washed bottle of distilled water, three slides, and Gram-staining reagents
The Gram stain is a system used to characterize bacteria based on the structural characteristics of their cell walls. A Gram-positive cell will stain purple if cell walls are thick and a Gram-negative cell wall appears pink. Most bacteria can be classified as belonging to one of four groups (Gram-positive cocci, Gram-positive bacilli, Gram-negative cocci, and Gram-negative bacilli) (Phenotypic analysis. (n.d.).
The unknown substance is probably a carbohydrate because it tested positive for starch which is a polysaccharide. This reaction also had very similar results as the Lugol’s test for potatoes which is a polysaccharide. Although the colors from the test for potatoes were not the same colors as the test for the unknown; the Biuret test had a slight color change and the Lugol’s test had a dramatic color change for both the unknown and potatoes. I am sure that the unknown was a starch, but the Benedict’s test for sugar was positive for the potatoes while the Benedict’s test for the unknown didn’t have a color change. The unknown probably did not have a color change for the Benedict’s test simply because there were not enough sugar present in the unknown for it to test positive. The Sudan IV Test for Lipids did not test positive for the unknown nor the potatoes because there isn’t a trace of lipids in starch. Based on my results, the unknown has a little protein, a lot of starch and no traces of lipids or
I identified the genus and species of an unknown bacterial culture, #16, and I applied the following knowledge of morphologic, cultural and metabolic characteristics of the unknown microorganism according to the laboratory manual as well as my class notes and power point print outs. I was given an incubated agar slant labeled #16 and a rack of different tests to either examine or perform myself; the tests are as follows: Gram Stain; Nutrient Gelatin Test; Carbohydrate Fermentation; Dextrose, Lactose and Sucrose; IMVIC tests; Citrate, Indole, Mythel-Red and Vogues Proskauer test; as well as a Urease and TSI Test. Materials and Methods/Results Upon receiving the Microorganism (M.O.) #16, I prepared a slide by cleaning and drying it. Then, using a bottle of water I placed a sterile drop of water on the slide and used an inoculating loop, flame sterilized, I took a small sample of the unknown growth in my agar slant and smeared it onto the slide in a dime sized circle and then heat fixed it for ten minutes.
The mixture for that table’s flask was 15 mL Sucrose, 10 mL of RO water and 10 mL of Yeast, which the flask was then placed in an incubator at 37 degrees Celsius. In my hypothesis for comparison #4 the measurements would go up again with every 15 min. intervals because of the high tempeture and also be higher that then Controlled Table’s measurements. Hypothesis was right for the first part but was wrong for the second part of the comparison, the measurements did increase in the table’s personal flask but the measurements did not get higher than the Controlled Table’s measurements, see chart below. In conclusion, I feel that the substitution of glucose for sucrose made the enzymes work just as hard as the Controlled Table’s flask but just not as much because sucrose was too strong for the enzymes to
A urinary tract infection is a very common infection that can happen to anybody. A urinary tract infection usually occurs when bacteria enters the urethra and multiples in the urinary system. The Urinary tract includes the kidneys, the thin tubes that carry urine from the kidneys to the bladder (ureters), and the main tube that carries the urine from the bladder (urethra). Women, men, and children are all immune to this infection. Women have the highest chances of getting it. In the Urinary tract, the main links of the ureters help get rid of any bacteria that tries to enter the urine, and the bladder helps prevent urine from backing up into the kidneys.
There were five test solutions used in this experiment, water being the control, which were mixed with a yeast solution to cause fermentation. A 1ml pipetman was used to measure 1 ml of each of the test solutions and placed them in separated test tubes. The 1 ml pipetman was then used to take 1ml of the yeast solution, and placed 1ml of yeast into the five test tubes all containing 1 ml of the test solutions. A 1ml graduated pipette was placed separately in each of the test tubes and extracted 1ml of the solutions into it. Once the mixture was in the pipette, someone from the group placed a piece of parafilm securely on the open end of the pipette and upon completion removed the top part of the graduated pipette.
stains on sputum’s and body fluids, and have completed a few AFB cultures. Apart from