Analysis of the Enzyme Sucrase for Optimum Temperature, pH, and Concentration ABSTRACT Enzymes react differently under different conditions and concentrations, being the most productive at the enzymes specific optimum condition and concentration. The enzyme sucrase, extracted from yeast, breaks down the complex sugar sucrose into the simple sugar glucose. Testing for sucrase’s optimum environment, multiple reactions were ran using varying amounts and concentrations of sucrose and sucrase at
Temperature and pH have large effects on the enzymatic activity of alpha amylase. This was determined by measuring the reaction rates by varying the temperatures and pH of a reaction. Figure 1 indicates that as the temperature increases the reaction rate increases. When the reaction rate reaches its maximum level this indicates the optimum temperature. The optimum temperature is where the enzyme preforms at its highest capacity. The optimum temperature in relation to this experiment was 37°C. This
6 ml of water in a test tube Ø Check the temperature of the water in the water bath with a thermometer that should be at 37°c, approximate body temperature. The temperature should be at 37°c as this is an optimum temperature range and if temperatures exceed this range too much the enzymes become denatured. This is because the enzyme loses its shape and gradually stops working. When the water in the water bath is at the required temperature of 37°c then Ø Add 2ml of amylase enzyme
Chemistry Investigation An investigation to find the optimum temperature that will produce the maximum amount of oxygen from hydrogen peroxide using the enzyme catalase Introduction Hydrogen peroxide is poisonous to all living things if it builds up in the cells but when it is introduced to the enzyme catalase the react between the two makes water and oxygen as byproducts. There are many factors witch affect the production of oxygen these are listed below. For this experiment the enzyme
Permeability of Beetroot Cell Membranes An experiment to investigate how temperature affects the Permeability of beetroot cell membranes Interpretation of Results: 0°C 20°C 30°C 40°C 55°C 0.24 0.28 0.75 0.79 0.22 0.26 0.35 0.41 0.81 From 0°C to 30°C there is a gradual increase of absorbency which shows that as the temperature increases it is denaturing the cell wall and cell membrane and allowing the beetroot pigment to leek out into the distilled water. As soon
glucose and fructose is carried out by the presence of the enzyme Sucrase. The activity of the enzyme will be greatest at its optimum temperature since at this temperature the enzyme's tertiary structure will be correct to allow the formation of the substrate-enzyme complex quickly and efficiently. This should be at 37°C (body temperature), which is the temperature of the ileum where Sucrase is secreted and naturally functions. The first thing to say about enzymes is that they are proteins
Investigating Amylase Aim: The aim of the experiment is to investigate how effectively the enzyme amylase breaks down starch at different temperatures, and therefore to find the optimum temperature that amylase digests starch. Introduction: Thousands of chemical reactions take place in our cells and those reactions need to happen quickly in order to keep us active. These chemicals are called ENZYMES. Enzymes make reactions happen at a much faster rate. Enzymes come in two main
for this practical are: Temperature - Increasing the temperature give the molecules more energy. Concentration of enzymes - The more enzyme there is, the more molecules there are working. PH - Different enzymes work best at different levels of pH. Concentration of substrate I have chosen to investigate temperature. I predict that raising the temperature will raise the rate of reaction until it reaches its optimum temperature. Raising the temperature beyond this point will lower
molecules at higher light intensities. Photosynthesis will reach a maximum rate when chlorophyll is saturated by sunlight. The Dark Reaction An increase in temperature will increase the rate of glucose production until it reaches the optimum temperature for the enzymes. The enzymes will be denatured if it goes above a certain temperature. An increase in carbon dioxide will also increase the rate of glucose production. Millions of years ago there was much more carbon dioxide in the atmosphere
Finding the Best Temperature for the Enzyme Amylase Aim: The aim for this experiment is to find out the best temperature for the enzyme amylase and at what temperature of water it works the fastest. I plan to collect evidence to prove that the enzyme amylase will work best at the temperature 37ËšC. I will choose a range of temperatures two below 37ËšC and two above 37ËšC so I will be able to determine what temperature it works best at. Prediction: I think that the enzyme amylase will
Gene expression is the ability of a gene to produce a biologically active protein. This process is regulated by the cells of an organism, it is very important to the survival of organisms at all levels. This is much more complex in eukaryotes than in prokaryotes. A major difference is the presence in eukaryotes of a nuclear membrane, which prevents the simultaneous transcription and translation that occurs in prokaryotes. Initiation of protein transcription is started by RNA polymerase. The activity
The Effect of Temperature on the Rate of Respiration in Yeast There are two types of respiration in yeast: Aerobic: [IMAGE] Anaerobic: Glucose [IMAGE] Carbon dioxide + ethanol + energy Respiration is controlled by enzymes, which are proteins which speed up one or more biological reactions. Within any cell many chemical reactions are going on at any one time. Yeast has many different types of enzymes that speed up respiration. Prediction I predict that as temperature increases, the
The Effect of Temperature on Enzyme Activity Experiment: To investigate the effect of temperature on enzyme activity. The enzyme used will be catalyse. The enzyme catalyse: The enzyme catalyse is a biological enzyme. It is used to break down Hydrogen Peroxide, which is harmful. The reaction that occurs is: [IMAGE] Hydrogen peroxide Water + Oxygen The activity of the enzyme catalyse can therefore be measured by the amount of oxygen (in the form
Protease Aim. To investigate the effect of temperature and the effectiveness of this enzyme in breaking down the gelatine (protein) on the back of photographic film. Prediction I predict that the effect of the temperature will be that the higher the temperature the quicker the enzyme will break down the gelatine, but the temperature will have to be an optimum temperature because if it is too high, the enzyme will be denatured. If the temperature is too low, the enzyme will not work and
molecule brakes down where the active site is. The substrate molecule splits and the enzyme is left unaffected. The factors that affect enzymes are: temperature, pH and surface area. Enzymes are used in everyday life because all living things produce enzymes. Saliva in a human produces a lot of enzymes for digestion. The optimum temperature for these enzymes to work in is 37.5 °C. Enzymes also perform best at a pH which is around neutral. When an enzyme reaches 40°C it becomes denatured.
The Effect of Temperature on the Rate of Reaction of Yeast Aim My aim is to view the effects of different temperatures on the rate of respiration of yeast in a glucose solution. I will do this by measuring the rate of which carbon dioxide is given off (CO2) by the yeast. Scientific knowledge ==================== From previous experiments I have learnt several things about yeast and enzymes. I have learnt that an enzyme is a natural catalyst, (something that speeds up the experiment
The Effect of Temperature on an Enzyme's Ability to Break Down Fat Aim: To investigate the effect of temperature on an enzyme’s (lipase) ability to break down fat. Hypothesis: The graph below shows the rate increasing as the enzymes get closer to their optimum temperature (around 35 degrees Celsius) from room temperature. The enzyme particles are moving quicker because the temperature increases so more collisions and reactions occur between the enzymes and the substrate molecules
Influence of Temperature on the Activity of Potato Catalase Hypothesis That the higher the temperature the higher the reaction rate of potato catalyse to a point were denaturing occurs in the enzyme and the reaction rate of the potato catalase drops off. Prediction The rate of Catalase activity will be faster at higher temperatures until a point, because at higher temperatures there are more chances of collisions between the enzyme's (Catalase) active site and the substrate (hydrogen
used as a mechanical work. This work can be manifest by bringing a working material from a high heat condition to a lower heat condition, by that the heat engine will produce calorific power that creates higher temperature conditions of the working substance. After generating a higher temperature state to the working substance a work will be p...
Daphnia are ectothermic animals which means their core body temperature fluctuates depending on the temperature of their environment. Temperature has a powerful effect on the biological processes of ectothermic animals. Acute changes to water temperature can have major impacts upon genetic growth and development as well as physiological and metabolic processes. Changes in temperature that remove Daphnia outside their optimum temperature range can affect processes such as oxygen transport, heart and