RResults represented in Table (1) indicated that, the amount of total protein in adult male more than other stages in black and white liquors larval treatments, in which they were 20.83 and 19.05 mg/g.b.wt, respectively. Black liquor larval treatment, pupal stage showed the lowest amount of total protein as compared with the other stages, which were 15.53 mg /g.b.wt. While white liquor larval treatment, adult female stage showed the lowest amount of total protein as compared with the other stages, which were 17.19 mg /g.b.wt. Table 1: Total protein contents in deferent developmental stages of Culex pipiens larval treatment with black and white liquors in comparison with non treated stages: Tested compounds Total protein in mg / g.b.wt. Total protein ratio* Larvae Pupae Adult female Adult male Larvae Pupae Adult female Adult male Black liquor 15.12 15.53 19.65 20.83 1.01 0.84 1.08 1.2 White liquor 16.29 19.05 17.19 19.05 1.09 1.03 0.94 1.1 Control 14.96 18.42 18.26 17.23 - - - - Total protein in treated stages * Total protein ratio = _______________________________________ Total protein in control Within a column, same latter mean no significant differences at 0.05 level of probability Within a column, different letters mean significant differences at 0.05 level of probability It is clear that the total protein contents in larval treated either with black liquor or with white liquor were higher than control in all developmental stages except in pupal stage Black liquor treatment was lower than control. The values of the total protein ratio in the larvae, pupae, adult female and adult male which treated with black liquor we... ... middle of paper ... ...ze fractionated into 29 bands There were three common bands between control group and White liquor treatment, bands No. 25, 27 and 28 (Mwt 21.4, 16.4 & 11.3 KDa, respectively) and two common bands between control group and Black liquor treatment, bands No.2 & 19 (Mwt > 203 & 38.4 KDa, respectively). Also, there were two common bands between both treatments, bands No. 6 & 23 (Mwt 94.4 & 25.5 KDa, respectively) Bands No. 4, 7, 9, 12, 16, 17, 20, 24 & 26 (Mwt 143.8, 88, 70, 63.4, 52.6, 48.8, 34.7, 25.2 & 19.4 KDa, respectively) appeared only in control group. Bands No. 1, 8, 11, 15, 18 & 21 (Mwt > 203, 83.3, 65.1, 55, 40 & 32.2 KDa, respectively) were detected only in White liquor treatment. Bands No. 3, 5, 10, 13, 14, 22 & 29 (Mwt 163.7, 122.4, 68.5, 59.1, 57, 26.7 & 5.5 KDa, respectively) were appeared only in Black liquor treatment as shown on Table (3) & Fig. (1).
The goal of this two week lab was to examine the stereochemistry of the oxidation-reduction interconversion of 4-tert-butylcyclohexanol and 4-tert-butylcyclohexanone. The purpose of first week was to explore the oxidation of an alcohol to a ketone and see how the reduction of the ketone will affect the stereoselectivity. The purpose of first week is to oxidize the alcohol, 4-tert-butylcyclohexanol, to ketone just so that it can be reduced back into the alcohol to see how OH will react. The purpose of second week was to reduce 4-tert-butylcyclohexanol from first week and determine the effect of the product's diastereoselectivity by performing reduction procedures using sodium borohydride The chemicals for this lab are sodium hypochlorite, 4-tert-butylcyclohexanone
..., Department of Zoology, Miami University, Oxford, OH, Available from Journal of Insect Physiology. (46 (2000) 655–661)Retrieved from http://www.units.muohio.edu/cryolab/publications/documents/IrwinLee00.pdf
Also, to see if there were different serotypes between the egg vs. egg content cultures. The eggs were sanitized as part of a control measure to see whether that can be a method for eliminating the Salmonella.
To begin the lab, the variable treatment was prepared as the Loggerlite probe, used to later measure oxygen consumption, warmed up for approximately 10 minutes. To prepare the variable treatment, 200ml of Sodium and Ammo-lock water was measured in a container and a pre-prepared “tea bag” of tobacco was steeped in the room temperature treated water until a light yellow color was visible. After preparing the tobacco solution the preparation for the live goldfish began as two beakers were filled with 100 ml of treated water. Each beaker was weighed before addi...
The crustacean Daphnia magna, maintained in water at room temperature, was obtained. The chemical compound used are five (5) fold dilution of one (1) molar aqueous nicotine, five (5) fold dilution of one (1) molar aqueous caffeine, 10 fold dilution of one (1) molar aqueous nicotine, five (5) fold dilution of one (1) molar aqueous caffeine, water and 100% alcohol.
Methionine represents the first limiting amino acid in broiler nutrition, thus different sources are available to balance diets based of corn and soybean. Bioavailability is different for each methionine source because of its rate of absorption and metabolic pathways. A broiler experiment was conducted to determine the relative bioavailability of Hydroxyl Methyl Analog Calcium (HMA-Ca) relative to DL-Methionine(DL-Met). The experiment was conducted at at Lavinesp (Unesp, Jaboticabal). It was used 1890 male broiler Cobb 500 of 21 days old, they were weighted and distributed homogeneously in a complete randomized design with 13 treatments and 7 replicates each. All birds fed either a basal diet deficient in sulphur amino acids, digestible methionine and cysteine (dig Met+Cys), or the basal diet with four levels of HMA-Ca (0.063, 0.183, 0.302 and 0.540%) and DL-Met (0.054, 0.156, 0.259 and 0.463%) to achieve increasing levels of dig Met+Cys. For the analysis, 5% of significance was considered and procedures of non-linear model were used by SAS. Exponential regression determinates bioavailability of HMA-Ca relative to DL-Met by calculating the relation of the slope of HMA-Ca relative to DL-Met
Throughout the history, people have often discussed the key elements that lead to the success of heterotrophic lives. One of the contributing factors, dietary intake, is regarded as the most essential among all. Although people recognize the importance of dietary intake, the effect of nutrition level of diet on various parameters of heterotrophs is poorly understood (Cruz-Rivera & Hay, 2000). Some of these biological attributes such as metabolism and fecundity allow organisms to achieve a higher Darwinian fitness, the likelihood of organisms to survive and reproduce (Sober, 2001). Thus, such an important relation between
Experiment #3: The purpose of this experiment to test the chromatography of plant pigments the alcohol test strip test will be used.
In osmosis, the proteins only on the surface are called peripheral proteins, which form carbohydrate chains whose purpose is used like antennae for communication. Embedded in the peripheral proteins are integral proteins that can either be solid or have a pore called channel proteins. -11.07% Discussion The chart shows the percent sucrose solution that resulted in the greatest change in average egg mass was the 0% solution, while the 1.73% solution resulted in the least change. The data also suggest that the percent sucrose solution that is isotonic to an egg’s interior may be between -10% and -15%, since the average masses of the eggs did not change beyond 0.15% over a 30 minute time period. In fact, the control group displayed the least amount of change in mass (0.10%) over a 40 minute period of time.
Random and systematic errors are both factors that can affect the reliability and accuracy of the results respectively. As all the graphs contained outliers, and hence, scatter, this indicates that random errors were present. Such errors may result from the inconsistent masses of the Alka Seltzer tablets. As these tablets were cut manually with a knife, it is unlikely that the mass of each half of an Alka Seltzer tablet would be the same. Thus, when using the tablets to react with HCl, the true number of tablets reacting would have not been the same as the number denoted for the trial, and with each repeated trial for the same number of tablets reacting, the reacting mass and ratio would have not been inconsistent. Consequently, the
We are testing the metabolic rate of a cricket and (Blaptica dubia) cockroach under physical stress, cold, heat, and lethal heat.
The conical vial was placed in a small beaker and allowed to cool to room temperature. The mixture was Cooled thoroughly in an ice bath for 15-20 minutes and crystals collected by vacuum filtration on a Hirsch funnel. The vial was rinsed with about 5 mL of ice water and transferred into to the Hirsch funnel and again washed with two additional 5mL portions of ice water. Crystals were dried for 5-10 minutes by allowing air to be drawn through them while they remained on the Hirsch funnel. The product was transferred to a watch glass plate and allow the crystals to dry in air. Crude acetaminophen product was weighed and set aside a small sample for a melting point determination and a color comparison after the next step. Calculation of the percentage yield of crude acetaminophen (MW = 151.2). was done and recorded in the lab notebook.
Poultry production is a very important and diverse component of Northern American agriculture. Poultry products including eggs, chicken and turkey meat have been an essential part of the diets of most Americans throughout history and other individuals all arou...
Potassium permanganate test is performed to see the presence of alcohol in this experiment. Five solutions were tested and out of all of them, aqueous methanol and solutions 4 and 6 changed color during the test, meaning that those solutions contained alcohol. Fresh aqueous aspartame and water do not contain alcohol, which is why we saw no color change. Solutions 4 and 6 were hydrolyzed aspartame and diet soda, respectively, under different conditions, and methanol is a product of hydrolysis.
The experiment measured the survival rate, the growth rate, and the size of the brine shrimp at the time harvested in various environments. To obtain these measurements, three environments were created: sea water, brackish water, and freshwater. For this experiment the scientists used 5 liter plastic buckets. Every two days, half of the water from each bucket was discarded and new water, of each respective salinity, was added into each bucket...