To achieve this, we need to perform the sequential comparison of the protein with other structurally existing proteins. Along with this, we need to purify the protein without any tags for forming crystals.
Now a days, the main problem in resolving the pathological issues is to pass the antibiotic through highly secured cell wall of the organism. The very important and the best example for this are the gram negative bacteria which has slight changes when compared to gram positive bacteria. These bacterial genes are obtaining high resistance towards the antibiotics due to the presence of an extra layer ‘Lipopolysaccharide’ (LPS) in the outer membrane(Erridge, Bennett-Guerrero et al. 2002). The inner membrane of the cell is composed of phospholipids whereas the outer layer composed with different lipids called Lipopolysaccharide’s. This LPS also known as Endotoxins(Wang and Quinn 2010) which has hydrophobic component Lipid A, and hydrophilic polysaccharides which are divided in to inner core, outer core and o antigen repeat sites(Wang and Quinn 2010). All these sites are composed of different components such as Kdo, Heptose, Galactose, Glucose and PPEtn’s. The contribution of these different compounds is shown in fig 1. Due to the presence of the extensive out...
... middle of paper ...
...ahne and S. Walker (2010). "The bacterial cell envelope." Cold Spring Harb Perspect Biol 2(5): a000414.
Sperandeo, P., G. Dehò and A. Polissi (2009). "The lipopolysaccharide transport system of Gram-negative bacteria." Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1791(7): 594-602.
Valvano, M. A., C. L. Marolda, M. Bittner, M. Glaskin-Clay, T. L. Simon and J. D. Klena (2000). "The rfaE gene from Escherichia coli encodes a bifunctional protein involved in biosynthesis of the lipopolysaccharide core precursor ADP-L-glycero-D-manno-heptose." J Bacteriol 182(2): 488-497.
Wang, X. and P. J. Quinn (2010). "Endotoxins: lipopolysaccharides of gram-negative bacteria." Subcell Biochem 53: 3-25.
Wang, X. and P. J. Quinn (2010). "Lipopolysaccharide: Biosynthetic pathway and structure modification." Progress in Lipid Research 49(2): 97-107.
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