The aim of the experiment is to investigate the effect of temperature
on the rate of respiration of yeast cells with glucose. As yeast cells
use up glucose in respiration, carbon dioxide gas is given off.
Measurements of the volume of carbon dioxide gas given off within a
set amount of time can be used to measure the rate of reaction. A fast
rate of reaction would be indicated by a large volume of carbon
dioxide gas being collected within this set amount of time. A small
volume of carbon dioxide gas collected within the same amount of time
would indicate a slower rate of reaction. The rate of reaction will be
fastest at the optimum temperature for working enzymes in yeast cells.
Procedure for preliminary experiment
List of apparatus
A packet of fast acting yeast
A tub of D-glucose
A rapid weighing balance
A 100ml measuring cylinder
A conical flask
A count down timer
A gas syringe
A delivery tube with rubber bungs tightly fitted at the ends joined to
the gas syringe
2 clamp stands
An electrical water bath
The equipment above was collected and set up as shown below and the
electrical water bath was set to 20ÂºC:
Â· 25g of yeast and 12.5g of glucose were measured out into a 100ml
beaker using a weighing balance.
Â· Using a measuring cylinder, 50ml of distilled water at room
temperature was measured.
Â· Pour this into the beaker of yeast and glucose.
Â· Dissolve the yeast, glucose and water together by stirring with a
... middle of paper ...
...ate of reaction will be slow giving me inaccurate results. I will
control this by trying to complete my experiment in one lesson so I
can use the same yeast each time).
We had to keep the beaker rapped in tin foil so that the temperature
would stay as close as possible to the temperature that we were
measuring. The tin foil acts as an insulator and keeps the heat inside
the same thermometer during the whole experiment the results would be
inaccurate, as I would have to wait for the thermometer to reach the
certain temperatures again.
I made sure that I used the same amounts of yeast accurately, so that
I could get the most accurate results. Upon looking back at the
adjustments made, I believe that I am confident in saying that, I made
sure that my experiment was as fair as I could possibly make it.
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