Isolation of Nuclei and Mitochondria from Cauliflower Florets by Differential Centrifugation

Isolation of Nuclei and Mitochondria from Cauliflower Florets by Differential Centrifugation

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Isolation of Nuclei and Mitochondria from Cauliflower Florets by Differential Centrifugation

Nuclei and mitochondria are both organelles that are found within most
eukaryotic cells. The nucleus contains most of the genes needed for
classification. It is "one of the most prominent structures to be
encountered in the eukaryotic cell" (Schwarz 24). Nuclei were first
observed by a Scottish plant taxonomist name Robert Brown in 1831. He
studying Orchidaceae and Asclepiadaceae at the time when he noticed a
structure in the cell that was consistent with much of the cells he
was viewing. He termed this "the nucleus" (Enersen 1). Later in the 19th
century scientists started using dyes to stain the nucleus. It was
found to be consistent in plant cells as well as cellular division
(Robert 1). The nucleus is enclosed by a double membrane nuclear
envelope. Inside the nucleus DNA is organized into material called
chromatin (Biology 117). Nuclei are the central part of the cell, the
densest organelle, and considered to be the brain of the cell.

Mitochondria are believed by many to be the result of a symbiotic
relationship between a bacteria and a primitive cell. This theory is
called "the endosymbiont hypothesis" (Schwarz 24). Some believe that
early bacteria invaded or were engulfed by a cell and instead of being
digested the bacteria formed a symbiotic relationship. Mitochondria
are involved in the catabolic process that generated ATP from sugars,
fats, and other forms of fuel. ATP, adenosine triphosphate, is an
organism's energy source. Living things convert fuels into ATP which
is handled easier by the creature. Mitochondria are enclosed in a

... middle of paper ...

...s feasible. The process of
differential centrifugation describe above is a worthy procedure to
use if small particles are needed to be isolated and observed. This
experiment illustrates how this process can be carried out as well as
provides data found on completion.

Works Cited

Biology (6th ed.). ed. Neil A. Campbell and Jane B. Reece. San
Francisco: Benjamin Cummings, 2002.

Enerson, Daniel Ole. "Robert Brown." Who Named IT?
. 1994-2001.

"Robert Hooke Describes Cells."

Schwarz, Otto J. "Introduction to Basic Laboratory Skills for the
Biological Sciences- An Experimental Approach." Oak Ridge, TN:
Performance Press, 2000.

Figure 1 Nuclear Diameters


Text Box: Nuclei observed[IMAGE]


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