Investigating the Effects of Sucrose Concentration on Osmosis in Potato Tissue

Investigating the Effects of Sucrose Concentration on Osmosis in Potato Tissue

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Investigating the Effects of Sucrose Concentration on Osmosis in Potato Tissue



PLANNING
========

We have been asked to research the effects of sucrose concentration on
osmosis in potato tissue. Osmosis is the spontaneous movement of a
solvent (-in this case water) from a place where its chemical
potential is higher to another place where it is lower, through a
semi-permeable membrane (-such as a cell membrane). A cell membrane
lets smaller molecules such as water through but does not allow bigger
molecules to pass through. The smaller molecules will continue to
diffuse across the membrane until the area in which the molecules are
found reaches a state of equilibrium, meaning that the molecules are
in a steady state, with molecules moving in and out of the membrane,
however with no net loss or gain.

Plant cells always have a rigid cell wall and cell membrane
surrounding them. When they take up water by osmosis the cells start
to swell up, but the cell wall prevents them from bursting, (-which is
what animal cells, who have no cell walls would do), the plant cell is
then said to be turgid. When turgid, the pressure inside the cell
rises and eventually the internal pressure of the cell is so high that
no more water can enter the cell. This hydrostatic pressure works
against osmosis, even when the plant cell's water potential is greater
than that of the outsides. Turgidity is very important to plants
because this is what makes the plant "stand up" and acts as a form of
support.

The opposite of turgidity is plasmolysis, this is the retraction of
the cytoplasm from the wall of a vacuolated plant cell as a result of
osm...


... middle of paper ...


...ETY

I will have to wear goggles to prevent the sucrose liquid getting into
my eyes, take care with the sharp scalpel, and to wear aprons to
prevent splashes getting on my uniform.

Preliminary work-shapes my understanding

The preliminary work let me decided on some crucial factors:

-Time for experiment: 10 minutes

-Amount of solution: 5ml

-Length of potato: 3cm-make sure no skin is on the potato, as it will
block the osmosis happening on that part of the potato, therefore
smaller surface area.

-Dry potato after experiment to get rid of excess water-see reasons
for this actions above

- Mixing beaker will be washed using water and dried using paper
towels-see reasons for this actions above

-Concentrations of sucrose to be used:

-0%

-20%

-40%

-60%

-80%

-100%

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