Factors that Affect the Rate of Enzyme Catalysis Essay

Factors that Affect the Rate of Enzyme Catalysis Essay

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Factors that Affect the Rate of Enzyme Catalysis

Enzymes exist in all living things. They are composed of polymers of
amino acids and are produced in living cells. Each cell contains
several hundred enzymes, which catalyse a vast number of chemical
reactions. Enzymes are known as Biological Catalysts as they
dramatically increase the rate at which reactions occur within living
organisms, without being 'used up' or effecting the reaction in any
other way. Enzymes catalysis saves the need for an increase in
temperature in order to speed up reactions within living things. Such
an increase in temperature would be lethal to the organism.

In this investigation I intend to explore the one of the factors that
effect the rate of enzyme catalysis. My research from textbooks and
the Internet suggests that this depends on several factors;
temperature, pressure, pH and concentration. After research and
careful consideration, I have decided to first look at how a change in
temperature could affect the rate of reaction.

In order to design a suitable experiment and make a credible
prediction, I must first explore more closely how temperature is
likely to affect the rate of catalysis. Enzymes are specific - they
only control one type of reaction; therefore I must use one specific
enzyme in my experiment, in order to find a clear way of measuring the
rate of reaction. Although they are specific, all enzymes work in a
very similar way and have similar properties. They are all globular
proteins and are all biological catalysts, they increase the rate of a
given reaction without being used up and their presence does not
change the nature of the re...

... middle of paper ...

...e was a success, but it could be improved by
the use of more accurate equipment and better organisation. Several
assumptions had to be made in this experiment. When such assumptions
are made, further work needs to be carried out to check these
assumptions. I had to assume that all enzymes worked in the same way,
but further work could be done with different enzymes and reactions to
check this. Through the experiment, I measured the rate of reaction at
10degrees intervals. I think that further work should be done between
30degrees and 40degrees in an attempt to find the exact optimum
temperature for the enzyme catalyse. Work between these temperatures
would allow me to plot a more accurate graph and explain the apparent
slowing in the increase of the rate of reaction between these two
temperatures in my current results.

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