Investigating the Rate of Transpiration in a Mesophyte Plant Essay

Investigating the Rate of Transpiration in a Mesophyte Plant Essay

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Investigating the Rate of Transpiration in a Mesophyte Plant Experiment to investigate the relationship between the number of
stomatal pores on the upper and the lower surfacesof the leaves of a
mesophyte plant and the rate of transpiration from those surfaces.

The aim of the experiment

The aim of the experiment is to investigate how the number of stomatal
pores is related to the rate at which water is lost from the leaves. A
mesophyte plant is chosen and the comparison is between the upper and
the lower surfaces of its leaves.

Experimental hypothesis

Taking into account the relative background scientific Information, it
is expected to be proven that the rate of transpiration from a leaf of
a plant is proportional to the number of stomatal pores on the surface
of that leaf.

Null hypothesis

Negative results would be to establish that the transpiration rate is
inversely

proportional to the number of stomatal pores or is not affected by it
whatsoever.

Introduction

Water is the universal solvent for a huge amount of chemical

substances in all living organisms. Plants require water for many
different reasons. It is used to uptake inorganic minerals from the
ground, to transport nutrients such as amino acids and carbohydrates
along their stems and to control their temperature. Water plays a very
significant role in the life cycle of plants being a vital assumption
for their life.

Plants take up water by the younger parts of the roots. Water then

moves across the cortex of the root towards the c...


... middle of paper ...


...ttach the blue cobalt
chloride paper on the leaf by a sellotape piece and start timing.

Observe the colour change of the cobalt chloride paper as water

evaporating from the leaf turns it pink from blue and for more
accuracy, compare with a moist sample. Stop timing as soon as the blue
colour is lost. Repeat the procedure for five times on each surface of
this leaf, then proceed to another leaf until all ten are examined.
Record the results into a table.

Using a calculator obtain random coordinates on upper and lower
surfaces of the leaves and apply a thin layer of nail polish on those
sample areas. Leave to dry for 10 to 15 minutes, then remove the layer
by means of forceps and transfer onto microscope slides. Count the
number of stomata on a fixed area under light microscope. Record the
results into a table.

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