The Concentration of Sugar in the Cell Sap of a Potato Cell's Vacuole Plan
In this experiment I am intending to investigate the concentration of
sugar in the cell sap of a potato-cell's vacuole. I will discover this
using my knowledge of the biological process of Osmosis.
Osmosis is the movement of solvent (usually water) through a
semi-permeable membrane separating solutions of different
concentrations. The solvent passes from a more concentrated solution
to a less concentrated solution until the two concentrations are
equal.
Many cell membranes behave as semi-permeable membranes, and osmosis is
a vital mechanism in the transport of fluids in all living organisms,
the transport of water from the roots up the stems of plants is just
one of these.
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Apparatus
[IMAGE]For this experiment I will need the following apparatus:
1. One large potato,
2. [IMAGE]Five glass beakers,
3. One measuring cylinder (50ml),
4. One potato corer,
5. [IMAGE]One dissection tile,
6. One scalpel,
7. One measuring scale (2dp)
8. [IMAGE]Ruler (1dp).
To discover the concentration of sugar in the cell sap I will place a
piece of potato, exactly 2.5cm in length, in a beaker of salt solution
(varying in strength) and leave it for 24 hours. If the piece of
potato increases in weight, the concentration of water in the solution
is too low, therein, if it decreases in weight the concentration of
water in the solution is too high. But if there is no change in weight
whatsoever then the concentration of water in the solution ...
... middle of paper ...
... weight because there is a high difference between the concentration of
water inside the cell and the concentration outside the cell.
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I believe the potato will greatly decrease in weight because water is
now at a much higher concentration inside the cell than outside.
Therefore, due to Osmosis, the water molecules will move from where
they are in high concentration to where they are in low concentration:
leaving the cells and entering the solution.
Collection of Results
To be sure that my results are collected and recorded in the correct
manner, one which is easy to both understand and compare, I have
constructed four results tables which can be seen overleaf. I have
crated a separate table for every experiment and one in which I can
record the average result of each aspect of those experiments.
Investigate how the Concentration of Sucrose Solution affects the Mass of the Potato Chip Aim To find out how the concentration of sucrose solution affects the mass of the potato chip left in the solution for one day. Water Potential: The water potential of a solution is a measure of whether it is likely to lose or gain water molecules from another solution. A dilute solution, with its high proportion of free water molecules, is said to have higher water potential than a concentrated solution, because water will flow from the dilute to the concentrated solution (from a high potential to a low potential). Pure water has the highest possible water potential because water molecules will flow from pure water to any other aqueous solution, no matter how dilute. Prediction: Osmosis is the passage of water molecules from a weaker solution to a stronger solution, though a partially permeable membrane.
Investigation of the Concentration and the Effect of Sucrose on Osmosis in Apple and Potato Tissues
Moisture is heavy, and thus it can change the results of the experiment, as we only want the weight of magnesium and the magnesium oxide.
For the preparation of Part 1, pieces were cut from the potato and were blended. The prepared suspensions were broken cells of the potato used as the extraction. The process of the suspension was the homogenization of the organism and later was centrifuged so that some of the substances reached the bottom (insoluble particles) and used the liquid as the enzyme(Schultz, 2006). The enzyme was brown colored known as the catechol oxidase, and that product can be used to measure the reaction rate using a spectrometer (Schultz, 2006). We started with twice the volume of the stock, so for the case of Part 1 was to begin with 6 ml of full strength enzyme. Two test tubes were used for the dilution. The first contained 5ml of buffered catechol and the
The Effects of Concentration of Sugar on the Respiration Rate of Yeast Investigating the effect of concentration of sugar on the respiration rate of yeast We did an investigation to find how different concentrations of sugar effect the respiration rate of yeast and which type of concentration works best. Respiration is not breathing in and out; it is the breakdown of glucose to make energy using oxygen. Every living cell in every living organism uses respiration to make energy all the time. Plants respire (as well as photosynthesise) to release energy for growth, active uptake, etc…. They can also respire anaerobically (without oxygen) to produce ethanol and carbon dioxide as by-products.
The experiment is aimed at giving a better understatement of osmosis process and the different conditions in which osmosis occurs.
Water Potential of Potato Cells Aim: To demonstrate the Water Potential of Potato Cells. Objectives: · To show the water potential of potato cells using various measured concentrations of a sucrose solution and pieces of potato. · To record and analyse data to verify observed results. · The method and procedure was carried out as per instruction sheet. Observations: The experiment shows that the lower the concentration of the sugar solution, in the Petri dish, the mass of the potato increased.
Investigating Osmosis in Potatoes Aim: Investigate the movement of osmosis through a selectively permeable membrane, in this case potato. Introduction: Osmosis is the movement of water through a semi permeable membrane. The water passes from an area of high concentration to an area of low concentration, until the two concentrations are equal in concentrations of water. Many cell membranes behave as semi permeable membranes, and osmosis is a vital part in the movement of liquids in living organisms, for example, in the transport of water from the soil to the roots in plants.
I am going to use a range of concentrations to enable me to get a good
Investigating The Effect Of Concentration Of Salt Solution On The Mass Of Potato Chips Aim: The aim of this investigation is to see whether the amount of salt solution affects the mass of the potato chip Hypothesis: In the solution below, water will diffuse from the dilute solution into the concentrated solution through the process of osmosis. Therefore the dilute solution level will fall and the concentrated level will rise Line Callout 2: Disapproved (is unable to go through the semi permeable membrane O Line Callout 2: Approved (is able to go through the semi permeable membrane P Salt solution Water [IMAGE] When a substance such as salt dissolves in water, the substance's molecules stick with some of the water molecules, so the concentration of the water molecules decreases. When the water molecules are the same concentration on both sides of a semi permeable membrane and salt is dissolved into one of them, osmosis will occur as shown in the diagram below.
there would be no flow of water into or out of the cell so the cell
second test tube also add 6 mL of 0.1M HCl. Make a solution of 0.165
4. Put each group of potato discs in one of the 6 test tubes and watch
with 0.0M (distilled water) and go up by 0.1M until I reach 1.0M and I
Determining the Concentration of the Cell Sap in Potato Storage Tissue Aim: To determine the concentration of the cell sap in potato storage tissue. By using Osmosis, determine what the sugar concentration of cell sap is. Prediction I predict that the potato segment in the distilled water will definitely gain in weight because the solution outside it has a much higher concentration of water then in the cell sap meaning Osmosis will occur and the potato segment take in water. I predict that 0.2M sugar solution will also gain weight because it still has quite a high concentration of water outside the potato. The potato in the 0.4M solution will gain weight but the potato in the 0.6M and 0.8M solutions will lose weight.