My Spectrum Of Spinach Absorbance Graph Essay

My Spectrum Of Spinach Absorbance Graph Essay

Length: 771 words (2.2 double-spaced pages)

Rating: Better Essays

Open Document

Essay Preview

In my spectrum of spinach absorbance graph, the greatest peaks were at 435 nm, 465 nm, and a smaller peak at 670 nm. These values correspond to the approximate values of visible light wavelengths as indigo (445 nm), blue (475 nm) and red (650 nm)[3], respectively. This indicates that the spinach extract is the most absorbent of these colours and frequencies. The lowest absorbances were between 500 nm - 630 nm , which covers green (510 nm), yellow (570 nm), and orange (590 nm) light [3]. Green is a color that is reflected, and not absorbed by the spinach and this is indicative of the green colour that it appears as.


The literature value for spinach iron content is listed as 2.71 mg Fe / 100 g spinach [2]. This is quite different from my experimental results, which yielded a value of 0.0682 mg Fe / 100g spinach. The data from my reliable source is not the value that all spinach leaves contain, and is an average. Some will contain more or less, however my value is quite contrasting, and it would lead me to believe that perhaps there was a source of error which created this disparity between values. The one major source of error that I recorded and realized was likely with the our measurements. When we pipetted the various amounts of liquids in the five test tubes, we followed the procedure, eliminated air bubbles, and ensured the equipment was operated in the correct fashion. The same measuring tools were always used, and all materials were ensured to be clean before use. However, I noticed a discrepancy between the levels of liquid in the five test tubes. This suggests there indeed was a mistake made in the pipetting that was not realized, which caused varying amounts of liquid to be placed into each test tube, and likely a major...


... middle of paper ...


...ks of beta carotene syncing up with those of chlorophyll a and chlorophyll b, it can not be certain that there is a presence of this in the spinach. From this, it can be concluded that there is a presence of chlorophyll a, chlorophyll b, and it is not certain if there was beta-carotene in the spinach extract.




Conclusion:


I was able to match my graph from my spectrophotometer my literature values [1], to determine the presence of chlorophyll a, chlorophyll b, but was not able to determine if there was a presence or absence of beta-carotene in my spinach extract. My experiment yielded a value of 0.0682 mg Fe / 100g Spinach, which was not consistent with the value observed in my literature values [2], due to likely sources of error in measurement. I am confident in the spectrometer results, however I do not believe that the iron values of my spinach were accurate.

Need Writing Help?

Get feedback on grammar, clarity, concision and logic instantly.

Check your paper »

Analysis of the Absorption of Green Light Versus Red Light Absorption in Spinach Leaves

- Analysis of the Absorption of Green Light Versus Red Light Absorption in Spinach Leaves The goal of the experiment was to determine if green light had less ability to absorb than red light in spinach leaves. This was done by separating the photosynthetic pigments (chlorophyll a, chlorophyll b, carotene and xanthophylls) from one another using paper chromatography. The separated pigments were then analyzed for their absorption spectrum using a spectrographometer. When the data was graphed it clearly showed the higher rate of red light absorption over green light....   [tags: Photosynthesis Experiments Science Biology Essays]

Better Essays
1427 words (4.1 pages)

Graph Theory: The Four Coloring Theorem Essay

- Graph Theory: The Four Coloring Theorem "Every planar map is four colorable," seems like a pretty basic and easily provable statement. However, this simple concept took over one hundred years and involved more than a dozen mathematicians to finally prove it. Throughout the century that many men pondered this idea, many other problems, solutions, and mathematical concepts were created. I find the Four Coloring Theorem to be very interesting because of it's apparent simplicity paired with it's long, laborious struggle to be proved....   [tags: Graph Geography Essays]

Better Essays
1566 words (4.5 pages)

The Diagnosis Of Autism Spectrum Disorder Essay

- Autism has a wide assortment of symptoms that affect individuals differently and to varying degrees. The concept of Autism Spectrum Disorder allows for an easier diagnosis of autism based on behavioral characteristics ranging from social interaction, communication skills, and repetitive patterns of behavior. The diagnoses for autism are solely based on behavioral characteristics, therefore, the judgment of the parents, results from behavioral studies, and ultimately, the opinion of the clinical expertise will diagnose the child with autism....   [tags: Autism, Asperger syndrome, Autism spectrum]

Better Essays
1043 words (3 pages)

Association Of Mercury And Autism Spectrum Disorder Essay examples

- Parwin Noorahmad English 302 Research Proposal Further Investigation Of Association Of Mercury and Autism Spectrum Disorder Autism spectrum disorder (ASD) is a development disorder that typically appears during early childhood (Burbacher and Shen). This is the result of a neurological disorder that affects the functioning of the brain. Which a significant number of the children experience a social and communication deficits and abnormal repetitive behaviors. It has been indicated that the mercury is a toxic metal that effect on human health....   [tags: Autism, Autism spectrum, Vaccine]

Better Essays
1428 words (4.1 pages)

Essay about The Spectrum of Marriages in The Canterbury Tales

- In The Canterbury Tales Chaucer portrays a wide spectrum of marriage from what can be traditionally seen as the worst to the best. Three of these tales, The Miller's, The Franklin's, and The Wife of Bath's, support this examination of what can constitute an ideal marriage. First in the Miller's tale is exposed what can be interpreted as the worst type of marriage. In this fabliau Chaucer exposes the problems of an older man marrying a younger women and gives the impression that this situation should not be desired in a marriage, “He was jealous and kept her on a short leash, / for she was wild and young, and he was old” (lines 38-39)....   [tags: spectrum of marriage as seen by Chaucer]

Better Essays
649 words (1.9 pages)

The Autism Spectrum Disorder ( Autism ) Essay

- Describing Autism is a challenge in part because we are still unsure of what causes it. To add to the confusion most recently the new Diagnostic and Statistical Manual of Mental Disorders, 5th Edition (DSM-5) by the American Psychiatric Association, has changed the diagnostic criteria, stating that currently “Autism Spectrum Disorder reflects a scientific consensus that four previously separate disorders are actually a single condition with different levels of symptom severity in two core domains....   [tags: Autism, Autism spectrum]

Better Essays
1028 words (2.9 pages)

Essay Autism Spectrum Disorder ( Asd )

- Autism Spectrum Disorder (ASD) is a bio-neurological disability that typically emerges in the first three years of age. The disorder impacts the development of the brain, and social and communication skills are impaired. Some children may be less delayed from the disorder, as in Asperger’s syndrome, while others are severely disabled. Males are four times more likely to be diagnosed with autism than females, and since the disorder was first diagnosed in the United States, the occurrence has climbed to one in 68 children (About Autism, 2015)....   [tags: Autism, Autism spectrum]

Better Essays
1251 words (3.6 pages)

Autism Spectrum Disorder ( Asd ) Essay

- Autism spectrum disorder (ASD) is a condition of a very complex brain disorder which affects different areas of the brain. It is characterized by abnormal social interactions, varied problems with communication, and restricted or repetitive patterns of behavior which are classified in both severity and character. Autism occurs in all different socioeconomic and ethnic groups and can affect all age groups. It is estimated that 1 out of 88 children will be diagnosed with some type of autism spectrum disorder by the age of 8, with males being 4 times more likely to some type of autism spectrum disorder than females....   [tags: Autism, Autism spectrum]

Better Essays
1387 words (4 pages)

Children With Autism Spectrum Disorder Essays

- Autism Spectrum disorder is a mental condition that begins in early childhood. It is difficult for this person to communicate and build relationships. It is possible the person will feel aggression, have a tendency to be alone, have a speech disorder, have problems paying attention, and cause them to be unaware of emotions. This disorder could cause a person not to want to be around people because they are embarrassed, but the more time spent with others they will not be embarrassed any longer. Constant interaction will cause both communicators to become more comfortable with one another....   [tags: Autism, Autism spectrum, Education]

Better Essays
815 words (2.3 pages)

Autism Spectrum Disorder Essay

- What is Autism Spectrum Disorder and how does it affect those who have it, both physically and psychologically. Autism Spectrum Disorder affects a substantial amount of the population. It is a complex, neurological spectrum condition that damages standard brain function, affecting the development of an individual’s communication and social skills. As a result, Autistic patients often experience repetitive behaviors, lack of spoken language, and may face amplified medical conditions. Although there is no present cure, treatment options, such as medication and therapeutic activities, are available to improve the patient’s quality of life....   [tags: complex, neurological spectrum condition]

Better Essays
687 words (2 pages)