Micropropagation has become a widely used technique for the large-scale and rapid propagation plants. Over the last 30 years, great strides have been made to develop and optimize micropropagation methods and culture media for the large-scale multiplication of a large number of plant species. However, despite the improved propagation efficiency, the method is still plagued with many problems, which limit in certain cases the profitability of the technique. Indeed, in vitro plantlets present many aberrations like, stomatal malfunction, poor epicuticular wax deposition, somaclonal variation, poor rooting, and hyperhydricity. Many species cannot be cost-effectively propagated by tissue culture due to these induced anatomical and physiological modifications (which lead to an excessive loss during hardening). These developmental problems stem from the particular conditions plants are cultured under. Indeed, the in vitro culture environment is typically characterized by low lighting, poor gas exchanges, high relative humidity and high mineral and sugar contents. Yet, even if plantlets are provided with all essential trophic elements, and are cultured under constant temperature and light, they still appear to suffer from many types of stress.
Over the years, sugar has been considered an essential component of the plant in vitro culture medium. However, in addition to its nutritional role, sugar regulates many important metabolic processes associated with plant growth and development (signalling functions). At the cellular level, sugars are essential for intermediary and respiratory metabolism and are the substrate for the synthesis of complex carbohydrates such as starch and cellulose. In addition, sugars supply the precursors for amino a...
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...e to understand the plant physiology by gaining a more global picture of the biochemical composition. In a seminal work, Roessner et al. (2000) used GC-MS to obtain a comprehensive metabolic profile of a singles extracts of soil or in vitro grown potato tubers. About 77 metabolites of various biochemical groups were detected and quantified at once. This analytical method proved to be powerful and allowed the simultaneous analysis of a large set of metabolite and revealed major differences between the tubers from different origin. Using the same technique, Jeong et al. (2004) showed that 64 metabolites accumulated differentially during the transition from sink to source of quacking aspen leaves two-thirds of which showed more than 4-fold changes in relative abundance. In this case, the metabolic profiling of three leaf stages yielded distinct biochemical phenotypes.
We used Brassica rapa, a fast plant the rapidly reproduces, in an experiment that we performed a monohybrid cross and a dihybrid cross. We hypothesized that in the monohybrid cross, the F2 generation of Brassica rapa will follow Mendelian inheritance patterns and show a phenotypic ratio of 3 anthocyanin positive to 1 anthocyanin negative. If the F2 generation follows Mendelian inheritance patterns, then out of 1105 plants, 828.75 will be anthocyanin positive and 276.25 will be anthocyanin negative. For the dihybrid cross we hypothesized that the F2 generation of Brassica rapa will follow Mendelian inheritance patterns and show a phenotypic ratio of 9 Anthocyanin positive dark green to 3 anthocyanin positive yellowish green to 3 anthocyanin
Modern biotechnology was born at the hands of American scientists Herb Boyer and Stain Cohen, when they developed “recombinant deoxyribonucleotide, (rDNA), [1] for medicinal purposes. Subsequently, biotechnologists started genetically engineering agricultural plants using this technology. A single gene responsible for a certain trait, from one organism (usually a bacterium) is selected altered and then ‘spliced” into the DNA of a plant to create an agricultural crop consisting of that...
Materials used in the experiment included 5-7 g of the potato tissue, 50ml of 2.0M phosphate buffer coffee filter and guaiacol dye.
The another devastating abiotic stress which is considered to be highly responsible worldwide for decreasing yield and quality of crop productivity is drought (Lambers et al. 2008 ; Moghadam et al .,2011; Mohsen Pourgholam et al.,2013 ; M. Farooq et al., 2012; Abolhasani and Saeidi, 2004 ; Monjezi et al., 2013).It harms plant growth and development and reduces crop growth rate and also affects biomass accumulation. Generally, in crop plants drought severely affects the cell division and expansion, elongation of root, leaf size, proliferation of root and inhibition of shoot growth (Sharp & Davies 1989; Spollen et al.,1993;Yamaguchi et al.,2010). Furthermore ,it also badly hampers all kinds of plant functions and physiological and biochemical traits such as mineral elements, carbohydrates, free radicals, ions, hormones, lipids, and nucleic acids (HongBo et al., 2005; Yasar et al., ; Moghadam et al .,2011,Mohsen Pourgholam et al,2013) .The transportation of nutrients from the roots to the stem severely get affected by drought as the rate of transpiration is reduced and damage of active transport and membrane permeability take place (Viets, 1972; Alam, 1999; Yasar et al ). Simultaneously, due to decrease in soil moisture, problem occurs with the low distribution of absorbed nutrients by the plant roots in the soil (Alam, 1999; Yasar et al ). More importantly, drought leads to rise in generation of reactive oxygen species (ROS) due to energy accumulation in stress condition of plants (Smirnoff 1993; Asada 2006; Waraich et al.,2011).Drought diminishes photosynthetic carbon fixation primarily through restraining the entrance of CO2 into the leaf or by reducing metabolism (Smirnoff 1993; Loggini et al., 1999; Ap...
The cultures were maintained at 25±20C under 16 hr illumination of 4000 lux intensity. The results are presented in Table 1, it can be seen from the data that pH of the medium had significant effect not only on regeneration frequency but also on number of shoots developed in each culture. Maximum 62.5±4.7 percent cultures in CoS 98259 and 67.3±4.9 percent in CoS 767 developed shoots at pH 6.0 while regeneration frequency was the lowest at pH 5.6. An increase in pH form 6.0 to 6.2 and 6.4 reduced the frequency of shoot regeneration from the callus (Table
The primary nutrients that plants require are carbon, hydrogen, and oxygen from air and water. Sugar has a different effect on animals, humans, and plants. In this experiment, we are testing the effects of sugar on the growth of wheatgrass. For this experiment, we used regular sugar that we put in our food and drinks. To test our hypothesis we are using two groups control and experiment group. The control plant received only water but the experimental plant received sugar but, both plants were placed in the same temperature and same amount of water. Our hypothesis was correct, experiment plant “sugar water” yield more plant growth than control plant “water”. This experiment shows the sugar water plant grow faster than water because of the average of both plants. The sugar water experiment plant had longer in length compared to water control plant.
Plant defences are those mechanisms employed by plants in response to herbivory and parasitism. According to Hanley et al. (2007), “the tissues of virtually all terrestrial, freshwater, and marine plants have qualities that to some degree reduce herbivory, including low nitrogen concentration, low moisture content, toxins or digestibility-reducing compounds”. The type of chemical defence may be species specific (Scott 2008). The defences that plants possess may be in the form of chemical production or in the form of physical defences such as thorns or spikes and even through reinforced, rigid leaves. “The compounds that are produced in response to herbivory can either have a direct effect on the attacker itself (e.g. toxins or digestibility reducers), or serve as indirect defenses by attracting the natural enemies of the herbivores” (Bezemer & van Dam 2005). This essay will focus on chemical plant defences and in particular the effects of terpenes, phenolics, nitrogen-based defences as well as allelopathy in plants.
...ince, there is a need to use for advanced novel methods of culturing plant to furnish new means for quickly propagating,conserving of endangered species and also introducing exotic plants. The production of high quality planting material of exotic nature propagated from vegetative parts through tissue culture has created new opportunities in global trading. The exotic plants are advantageous for farmers;growers; nursery owners & rural employment. As exotic plants are restricted to their natural environment; the main benefit of tissue culture technology lies on production of high quality & uniform planting material that can be multiplied on a year round basis. The plant selected for such purpose is Stevia rabuadiana Bertoni. Objectives of study:
Photosynthesis is a process in plants that converts light energy into chemical energy, which is stored in bonds of sugar. The process occurs in the chloroplasts, using chlorophyll. Photosynthesis takes place in green leaves. Glucose is made from the raw materials, carbon dioxide, water, light energy and oxygen is given off as a waste product. In these light-dependent reactions, energy is used to split electrons from suitable substances such as water, producing oxygen. In plants, sugars are produced by a later sequence of light-independent reactions called th...
Tissue culture allows for the clonal propagation of plant (production of multiple copies of the same genotype).
In this experiment, researchers used different measurements of catechol and 1cm of potato extract. Researchers hypothesized that the increase in substrate would level out the enzyme activity by
This lab attempted to find the rate at which Carbon dioxide is produced when five different test solutions: glycine, sucrose, galactose, water, and glucose were separately mixed with a yeast solution to produce fermentation, a process cells undergo. Fermentation is a major way by which a living cell can obtain energy. By measuring the carbon dioxide released by the test solutions, it could be determined which food source allows a living cell to obtain energy. The focus of the research was to determine which test solution would release the Carbon Dioxide by-product the quickest, by the addition of the yeast solution. The best results came from galactose, which produced .170 ml/minute of carbon dioxide. Followed by glucose, this produced .014 ml/minute; finally, sucrose which produced .012ml/minute of Carbon Dioxide. The test solutions water and glycine did not release Carbon Dioxide because they were not a food source for yeast. The results suggest that sugars are very good energy sources for a cell where amino acid, Glycine, is not.
Cloning will open you to the risk of a fungus or pests wiping out the whole crop, so it's important to pick plants that exhibit great resistance to fungus and pests. Pick the plant you feel will be the most reliable to reproduce in large scale, based on health, growth rate, resistance to pests, and potency. The quality of the high, and the type of buzz you get will be a very important determining factor.
For many years, nature has cloned organisms. When a plant sends out a stalk and it takes root, the new ...
Plant nutrition is area of plant biology that is of the utmost importance for the proliferation of plants. Without proper nutrition, plants would simply cease to exist unless drastic alterations were made. There are certain elements that are required for the plant to grow and reproduce; these elements are known as essential elements. There are three requirements of an essential element: the element must be required for the completion of the plant’s life cycle, the element must not be replaceable by another element in whole, and finally the element must be direction involved in the metabolism of the plant. Chemical compounds that are involved in proper nutrition have been designated as nutrients, and further classified as macronutrients and micronutrients. Macronutrients are needed for growth, metabolism, and many other functions, but are designated as “macro” because they are required in larger amounts. Macronutrients include carbohydrates, proteins, and fat molecules. Micronutrients have a much wider function that depends on the exact micronutrient. Micronutrients are designated as so because they are needed in much smaller amounts when compared to macronutrients. Examples of micronutrients include vitamins and minerals.