Plant material
The seeds of A. precatorius were collected from the medicinal plant garden of Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, M.P., India. Seeds were sterilized and germinated by following the protocol described in our previous publication .[15]
Initiation of A. precatorius cell cultures
Different explants from aseptically germinated seeds viz. leaves, epicotyle and petiole were tested for culture initiation by variation in plant growth regulators (PGR) and Agrobacterium mediated transformation. Non-transformed callus cultures were initiated by placing explants on solidified MS medium supplemented separately with the hormones: 1 mg/l naphthalene acetic acid (NAA); 1 mg/l Kinetin (Kn); 0.5 – 2.0 mg/l 2, 4- dichlorophenoxy acetic acid (2, 4-D) and there combinations (Data not shown).
For transformation experiments, leaves were excised from 30 d old in vitro germinated plantlets of A. precatorius. A. tumefaciens strains (MTCC 431, MTCC 609, MTCC 2250 and MTCC 2251) were used to establish transformed callus cultures. These strains were procured from Microbial Type Culture Collection (MTCC), Institute of Microbial Technology (IMTECH), Chandigarh, India. A minimum of 30 explants were used for each experiment. All explants cultured on sterilized petriplates comprising MS medium solidified with 1.0 % agar and supplemented with 30 g/l sucrose. The pH was adjusted to 5.7 ± 0.2. The medium was autoclaved under 15 psig pressure at 121ºC for 20 min.
The explants were co-cultivated with Agrobacterium strains for infection to induce transformed callus. For this purpose, Agrobacterial colonies were cultured for 48 h on solid nutrient agar medium at 28 ± 2°C. Ten loopful bacteria were then...
... middle of paper ...
... in a maximum synergistic promotion of glycyrrhizin accumulation i.e. 4.9-fold higher compared to transformed control culture. The present study indicates the potential of these biotechnology-based methodologies for large-scale production of glycyrrhizin. Furthermore, in order to develop a process for commercial production of glycyrrhizin by plant cell cultures some additional yield enhancement strategies may be worked out like, optimization of medium composition, environmental condition and addition of precursors.
Acknowledgments The authors are thankful to Dr. Ashish Baldi, Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Hauz Khas, New Delhi, India for his valuable and timely assistance. The author VSK wishes to acknowledge All India Council for Technical Education, New Delhi for providing junior research scholarship.
However, at 3% substrate concentration, the hydrogen peroxide decomposition showed an immediate peak of up to 3.8 mm in height. As the substrate concentration slowly increased, enzyme
In this experiment the heat shock method will be used to deliver a vector (plasmid) of GFP to transform and grow E. coli bacteria. Four plates containing Luria Bertani (LB) broth and either –pGLO and +pGLO will have E. coli bacteria added to it. The plate containing –pGLO (no pGLO) and LB will show growth as ampicillin will be present killing bacteria but no glowing because no arabinose will be present for glowing to be activated, the same result will be seen in the plate containing +pGLO, LB and ampicillin. The plate with –pGLO, LB and ampicillin will show no growth and no glowing as no arabinose is present for glowing to be activated
How does the vegetation surface type affect the amount of runoff? Speculate why this happens.
...to plant, Solanum lycopersicum, conferring disease resistance has also been shown to be beneficial to health, as the modification results increased nutrient content, antioxidant activity, and lycopene concentration [3]. By insertion of rolB gene from A. rhizogenes into tomatoes, there are beneficial effects. Though this is one case of success, it is definite that researchers can eventually research and produce successful products of biotechnology.
Michael P. Broadribb, C. (2006). Institution of Chemical Engineers . Retrieved July 26, 2010, from IChemE: http://cms.icheme.org/mainwebsite/resources/document/lpb192pg003.pdf
This experiment was performed to test two hypotheses concerning the plant hormone gibberellic acid and a mutant rosette shaped phenotype of the plant Brassica rapa. This experiment was done in order to test the effects o gibberellic acid on plants and its effect on rosette shaped complexes. The two hypotheses in this experiment are as follows: Hypothesis number one states that Gibberellic acid allows for stem elongation in plants. Hypothesis number two. The rosette complex in the rosette phenotype plant contains less gibberellic acid naturally and therefore grows shorter.
Purpose: The purpose of this lab is to explore the different factors which effect enzyme activity and the rates of reaction, such as particle size and temperature.
...ince, there is a need to use for advanced novel methods of culturing plant to furnish new means for quickly propagating,conserving of endangered species and also introducing exotic plants. The production of high quality planting material of exotic nature propagated from vegetative parts through tissue culture has created new opportunities in global trading. The exotic plants are advantageous for farmers;growers; nursery owners & rural employment. As exotic plants are restricted to their natural environment; the main benefit of tissue culture technology lies on production of high quality & uniform planting material that can be multiplied on a year round basis. The plant selected for such purpose is Stevia rabuadiana Bertoni. Objectives of study:
Man has used hallucinogenic plants for thousands of years, probably since he began gathering plants for food. The hallucinogens have continued to receive the attention of civilized man through the ages. Recently, we have gone through a period during which sophisticated Western society has "discovered" hallucinogens, and some sectors of the society have taken up, for some reason or another, the use of such plants. This trend may be destined to continue.
...icot stem. Label and sketch its parts. Take note of the position of the vascular bundle.
Tissue culture allows for the growth of a plant without the use of seeds or pollination.
Bamboo is a widely used and it is an important material that is found typically anywhere in the world. It is generally found in rural areas as building materials in small tropical countries, this is due to bamboo having a high strength and low weight. Even though bamboo is referred to as a rural material, Architects and Engineers have begun to incorporating bamboo into buildings for strength and beauty. Bamboo is a versatile, renewable and sustainable resource in South Africa. It is easy to use bamboo in buildings, as simple tools can be used bend and cut it easily. Bamboo construction is also very resilient to wind, easy to build with and is even resistant to some natural disasters like earthquakes (if done and used properly). Bamboo is also used in landscape industry, interiors and as a temporary building material in construction.
Keener, K., Hoban, T. and Balasubramanian, R. 2014. Biotechnology and its applications. [online] Available at: http://www.ces.ncsu.edu/depts/foodsci/ext/pubs/bioapp.html [Accessed: 11 Apr 2014].
For many years, nature has cloned organisms. When a plant sends out a stalk and it takes root, the new ...
The development of new technologies for the stable and transient expression of recombinant proteins in plants could potentially provide more versatile biotechnological tools for the scientific and commercial communities (trends in plant sciences Vol xx no xx Month 2005)