Photosynthesis is the process in which chloroplasts found in plants, algae and some bacteria are used to convert light energy to chemical food energy. (Reece, et al. 2012, p. 190) (Molecules, Genes & Cells Laboratory Manual 2016).
Photosynthesis can be broken down into two parts, light dependent reactions and light independent reactions or Calvin Cycle. Light dependent reactions use light energy collected by the thylakoid to convert NADP+ and ADP to NADPH and ATP respectively (Cloot 1994, p. 23). This process is shown in figure 1. In the Calvin cycle which is light independent, CO2 is converted to glucose by using the energy of ATP and NADPH (Reece, et al. 2012, p. 192). The rate of photosynthesis can be determined using the amount of oxygen produced in an amount of time. An oxygen electrode is able to do this (Molecules, Genes & Cells Laboratory Manual 2016).
Respiration is the process in which glucose is broken down by the mitochondria to obtain the energy in its bonds so that is may be used in other metabolic processes. Respiration occurs in all living organisms and is separate from photosynthesis (Molecules, Genes & Cells Laboratory Manual 2016). Oxygen is consumed in this process and carbon dioxide is one of the products (Reece, et al. 2012, p. 169-184).
Diuron is a herbicide that will be used in this experiment. Diuron inhibits photosynthesis as found in a study by Harrington, et al. (2005).
The hypothesis being tested is that as the light intensity is increased the rat...
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...ired for the highest rate of photosynthesis. This would mean that plants will be in the best conditions when they are exposed to high levels of light and high levels of CO2. The presence of herbicides such as Diuron will prevent photosynthesis so they should not be present. It would be preferable for plants to grow tall or to have their leaves up high so that their chloroplasts may be exposed to the most light. Plants should not be bunched together as they will have to compete for resources. Being covered by shelter or other plants will prevent light from hitting the chloroplasts. Plants would photosynthesise the best in summer as they are exposed to the highest amount of light energy and would be lowest in Winter when there is cloud cover. Being placed higher on a mountain may be more beneficial however it is unknown how a plant may react to different temperatures.
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