Investigating The Effect Of Different Clothing Materials On Uv And Wild Type Yeasts With Uv Radiation

Investigating The Effect Of Different Clothing Materials On Uv And Wild Type Yeasts With Uv Radiation

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Discussion
This experiment was conducted to investigate the effect of different clothing materials on UV protection by exposing UV-mutant and wild type yeasts with UV radiation. We hypothesize that wool has the best UV protection due to its relatively heavier weight compared to aluminum foil, cotton and silk. If the hypothesis is supported by our data, then the remaining yeast cells in the wool-covered plates will be the most. After the UV exposure of all the yeast plates, we found that silk has the highest survival rate for UV-mutant yeasts, which is 89.99%, and cotton has the lowest survival rate of 28.57% for UV-mutant cells. However, the untreated sides for all UV-mutant yeasts have no trace of yeast colonies due to their lacking of DNA repair mechanism and covering materials to protect them. Although wool ranked 2nd among the four materials we tested on UV-mutant yeasts, the survival rate is still 39.88% lower than that of silk. Therefore, our hypothesis that assumes wool has the strongest UV protective capacity was negated by the data that silk has a higher survival rate of UV-mutant yeast cells. Some possible reason that the result was different from our hypothesis are as follows. First, human error such as failing to successfully cover the petri dishes with different materials can be a likely explanation. For example, the survival rate of trial 1 and 2 of aluminum foil had a drastic 32.93% difference. Next, the sample size with only 2 replications might not be able to represent the whole population. Third, the color difference between each material might affect UV absorption ability. For instance, black wool might absorb more UV compared to white cotton. Last, the thickness of different materials might affect the depth tha...


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...s through ozone layer, while all UV-C radiation is blocked. Although we shortened the exposure time for UV-C, if we use UV-A or UV-B, it can represent the surrounding environment better. Consequently, we can simulate our environmental UV radiation by treating our experiment with UV-A or UV-B rather than UV-C in the future.
In summary, for our future experiments we should control other variables in order to get more accurate conclusions for the best UV protective clothing materials. Nevertheless, from the result we had, we still can see that silk has the strongest UV protection ability among aluminum foil, cotton, and wool. Furthermore, in future experiments more fabrics such as polyester and the so-called “UV-protective” shirts claimed by some clothing manufacturer can be tested to find out which type of materials can radically block the DNA damaging UV radiations.

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