One of the most important parameters of sperm function is hyperactivation. For fertilisation to occur, spermatozoa must undergo capacitation either in vivo (in the female reproductive tract) or in vitro (in conditioned culture medium), which involves a sequence of membrane and metabolic changes, including transition of progressive motility to a highly irregular movement (hyperactivation). Hyperactivated motility is displayed by sperm swimming in the oviduct and has several physiological advantages, which could certainly help sperm to move effectively through different obstacles in the female reproductive tract e.g....
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...rtilisation rate was studied in prepared sperm samples that were surplus following treatment, to eliminate inter ejaculate-variation. The aims of this study were to examine (1) the incidence of Ca2+ store failure among sub fertile patients and its clinical significance in male infertility; (2) the relationship between % hyperactivation and intracellular Ca2+ level in response to Ca2+ -store mobilising agents; (3) if intracellular Ca2+ and HA are related to IVF success; (4) if hyperactivation in response to Ca2+ -store mobilising agents is biomarker to differentiate between men with normozoospermic samples and patients with severe male factor infertility; and (5) if impaired store mobilisation is stable problem in these patients or vary between ejaculates, this is achieved by recalling sub fertile patients with store malfunction to be examined further.
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