Essay on Identifying an Unknown Microbial Organism

Essay on Identifying an Unknown Microbial Organism

Length: 1644 words (4.7 double-spaced pages)

Rating: Powerful Essays

Open Document

Essay Preview

Identifying an Unknown Microbial Organism

The purpose of identifying an unknown microbial agent is so that the five “I’s” of Microbiology, which are, Inoculation, Incubation, Isolation, Inspection and Identification can be practiced. Providing an unknown microbial agent tests the ability of ones skill of the above techniques and to accurately obtain the correct results and compare these results to biochemical test results that are already established. (Forrest & Elliott, 2012, p.111)
Test used include the Gram Stain, the Streak Plate and Biochemical test such as the MR-VP, SIM, Phenylalanine Deaminase, Simmon’s Citrate along with the Carbohydrate Fermentation and Urease tests.
Biochemical Test of Importance
Several Biochemical test were performed, however, only three will be three will be discussed due to these test being the most significant in the identification of the microbial agent. These test helped narrow down the options of which organism the results matched with. The tests, which will be further dissected, are the SIM, Simmon’s Citrate and the Urease tests.
First, the SIM test consisted of Sulfur, Indole, and Motility; however, the focus will be narrowed to the Sulfur portion of the test only due to it being the most significant. Two methods used in this portion of testing were direct testing on the specimen and culture/Isolation. During the SIM testing, aseptic technique was cautiously used. An inoculation needle was used to obtain culture. Afterwards, the needle was taken and stabbed into the SIM media. The media was then stored at 37 degrees Celsius for 24-48 hours. (Forrest & Elliott, 2012, p.114)
The Sulfur portion of the SIM test consisted of the enzymes Thiosulfate reducta...

... middle of paper ...

.../Academic Press.
Cowan, M. K., & Talaro, K. P. (2009). Infectious Diseases Affecting the Respiratory System. In Microbiology: A systems approach (3rd ed., pp. 645-54). Boston, MA: McGraw-Hill.
Forrest, S., & Elliott, M. (2012). Lab 16 Identification: Biochemical Tests. In Microbiology: An Introduction to Laboratory Procedures & Techniques (pp. 111-116). El Dorado, KS: McGraw-Hill Companies, Inc.
Forrest, S., & Elliott, M. (2012). Lab 20 Identification of an Unknown Bacteria/ Published Results. In Microbiology: An Introduction to Laboratory Procedures & Techniques (pp. 135-138). El Dorado, KS: McGraw-Hill Companies, Inc.
PODSCHUN, R., & ULLMANN, U. (1998). Klebsiella spp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors. Clinical Microbiology Reviews. Retrieved from

Need Writing Help?

Get feedback on grammar, clarity, concision and logic instantly.

Check your paper »

Description of the Concept of the Microbial Loop Essay

- The concept of the microbial loop first began in 1926 by Vernadskii, who studied heterotrophic and phototrophic microbial metabolism; and understood that these systems represented a major part of total metabolism in the oceans (Pomeroy, 1988). Older techniques that scientists used for enumerating marine bacteria were by plate counts, serial dilutions and phase-contrast microscopy. These numbers represented about 10% of actual numbers and are no longer used (Azam et al, 1983). Scientists were unable to completely understand the microbial loop until recently when ultrafiltration techniques, applied electronic microscope techniques; and genomic techniques were developed to quantify biomass in...   [tags: marine bacteria, oceans, microbial loop]

Powerful Essays
2501 words (7.1 pages)

Identifying Unknown Subtances Essay

- Identifying Unknown Substances The goal of Project 11 is to identify an unknown substance that we were given. This project was split up into 2 weeks. The first week my group and I identified our substance by going through multiple tests like: a solubility test, conductivity tests, pH tests, anion test, and cation test. For the second week we had to confirm our unknown substance. For the solubility test we were told to try and dissolve our unknown substance into water (H20), Sodium Hydroxide (NaOH), Hydrochloric Acid (HCl), Toluene, and Acetone....   [tags: group project, test, chemistry]

Powerful Essays
1778 words (5.1 pages)

Essay about Experimental Design : Identifying An Unknown Insert

- Experimental Design #3: Identifying an Unknown Insert Purpose: The purpose of this experiment is to identify an unknown insert DNA by using plasmid DNA as a vector to duplicate the unknown insert DNA. The bacteria will then be transformed by having it take in the plasmid DNA, which will allow us to identify our unknown insert as either the cat gene or the kan gene. Experimental Design: The two modes of analysis that will be used to identify an unknown insert piece of DNA would be plating the transformation cells onto LA plates that have either ampicillin or chloramphenicol and PCR....   [tags: Molecular biology, DNA, Restriction enzyme]

Powerful Essays
2195 words (6.3 pages)

Investigation Of Identifying Unknown Broth Cultures Essay

- In class we was given the task of identifying unknown broth cultures, I received number 69. I went through several tests to figure out what bacterium I received. First I created a slide of the broth by putting a small amount of the broth on the slide and letting it dry for ten minutes. Next I stained the slide by applying the reagents; crystal violet, grams iodine, decolorizer and safranin. From that I discovered that this bacterium was gram-negative, this would determine the next couple of tests I would do to identify my unknown bacterium....   [tags: Escherichia coli, Bacteria]

Powerful Essays
1094 words (3.1 pages)

A Experiment Towards Identifying Our Unknown Was The Gram Staining Experiment

- Methods Our first experiment towards identifying our unknown was the gram staining experiment. During this procedure we had to eat fi the bacteria to the microscope slides, and use various stains, such as crystal violet, gams iodine 95% ethanol and safranin. The purpose of this staining technique was to find out the cell-wall composition of the unknown bacteria. The cell could’ve been either gram-negative or gram-positive. Gram- negative cell walls are shown as pink cells under the microscope because they have a thin layer of peptidoglycan....   [tags: Bacteria, Staining, Microbiology]

Powerful Essays
1588 words (4.5 pages)

Identifying Five Unknown Chemicals Essay

- Identifying Five Unknown Chemicals I was given 5 unknown samples, which are sodium chloride, sodium thiosulfate, calcium carbonate, sodium bicarbonate and sodium nitrate and these samples are all white and solid. However, I don’t know which substance is which sample and I was only given some information about the physical and chemical properties of these substances. In order to identify the 5 unknown samples, knowing which sample is which substance, I have to carry out this experiment, finding out the chemical and physical properties of these samples....   [tags: Papers]

Free Essays
1246 words (3.6 pages)

Identifying an Organic Unknown Essay

- Identifying an Organic Unknown Aim: In this investigation I will get a number of unknown organic compounds. Which i have to identify by using a number of different chemical and physical techniques. The only thing is known about the compound is that it contains one of the functional group: alcohol, aldehyde, ketone, carboxylic acid, phenol and ester. Therefore I devised a sequence of tests that would identify which one of the groups the sample contains. The flow chart below shows the chemical techniques, which I will use to identify the functional group of the unknown....   [tags: Papers]

Free Essays
819 words (2.3 pages)

Identifying an Unknown Analgesic Essay

- Identifying an Unknown Analgesic Objectives: 1. To identify a unknown analgesic 2. To identify the analgesic you will be given 4 known analgesics compounds. Each of which can be separated using by Thin Layer Chromatography. 3. Using thin layer chromatography the unknown analgesic will be compared to the 4 known analgesics. Spotting of the sample 1. Prepare a microcapillary tube for each analgesic and each standard 2. Immerse the small end of the tube into the sample vial until some of the sample is drawn into the tube....   [tags: Papers]

Free Essays
900 words (2.6 pages)

Identifying Two Unknown Species of Bacteria Essay

- Identifying Two Unknown Species of Bacteria Materials and Methods Week 1, Day 1 (10 November 2000) The first day an unknown sample was assigned to each group of students. The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The morphology of the two types of bacteria was viewed under the microscope and recorded. Then the sample was put on agar plates using the quadrant streak method for isolation. There were three agar plates; one was incubated at room temperature, the second at 30 degrees Celsius, and the third at 37 degrees Celsius....   [tags: Escherichia Coli Staphylococcus Biology Essays]

Powerful Essays
1333 words (3.8 pages)

Microbial Diversity Essay

- Micro- and macro-organisms are habitually associated with interactions shaping contrasting environments between different host-microbial communities {{59 Hughes-Martiny, J.B. 2006;}}. These interactions are microbial dominated as microbes outnumber host cells by many orders of magnitude {{68 Savage, D.C. 1977;}} and provide metabolic functions lacking from the host {{69 Gill, S.R. 2006;}}. Naturally occurring populations can also include interactions between host-pathogens colonization {{16 Critzer, F.J....   [tags: Micro-Ecology]

Powerful Essays
1951 words (5.6 pages)