How Light Intensity Affects The Reaction Rate Of Photosynthesis Essay

How Light Intensity Affects The Reaction Rate Of Photosynthesis Essay

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In the light intensity experiment, tubes of buffer solution, chloroplast, water and DPIP were placed in different intensities of light to determine how light intensity affects the reaction rate of photosynthesis. The reaction rate was measured by absorbance values in five-minute increments for 30 minutes. The tubes were placed at 24 cm away, 30 cm away, 49 cm away, and completely dark. The tube that was closest to the light had the smallest absorbance value, while the dark tube had the highest value. As the light intensity increases, the absorbance value decreased. In the action spectrum experiment, 10 aspirated spinach leaves and sodium bicarbonate solution were mixed together in beakers then each beaker was placed in different light boxes. The light boxes were blue, green, red, white and dark. The Photosynthetic rate was measured by the number of floating spinach leaves in the solution at five minute time increments. The red light had all floating disks at the end of 30 minutes, he green light had 6, and the blue light had 3. Thus the red light, wavelength of 680 nm, is the most effective for photosynthesis. In the absorption spectrum experiment, four different pigments were tested: carotene, chlorophyll a, chlorophyll b and xanthophyll. Each of the solutions, and their blanks, were put into the spectrometer at various wavelengths we continued increasing by increments of 50 nm each trial. The wavelengths from 400 nm to 500 nm had the best absorbance rates
Plants use photosynthesis to convert solar energy into chemical energy. The rate of photosynthesis can be determined indirectly by DPIP, which is a dye that essentially mimics coenzyme NADP, which becomes reduced when an electron is added to NADPH during light reactions (Lab...


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...rmines what wavelengths of light can be absorbed. Plants that are green normally contain chlorophyll a as well as b. These chlorophylls, absorb different types of wavelengths, but don 't absorb ones that are green. These plants are green because that wavelength of light is reflected instead of absorbed. Different colors of light can affect how much photosynthesis occurs, because only certain colors are absorbed. The more color that’s absorbed, the more light that’s absorbed, which leads to more photosynthesis. Our experiment demonstrated that the red wavelength was the most effective for photosynthesis behind white, which was our positive control due to the fact that it contains all the colors of the spectrum. In our final experiment for the absorption spectrum our results supported our hypothesis and the absorbance levels decreased as we increased the wavelength.

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