I selected this article for the midterm assignment because I was interested in learning more about research with human induced pluripotent stem cells and cell replacement therapy after the class session and paper discussion with Dr. Melissa Wong about intestinal stem cells. In addition to introducing a new method aimed at iPSC replacement therapy, the authors also test their method against several potential problems that could prevent it from being clinically applicable. Overall,...
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1. Yusa, K. et al. Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells. Nature 478, 391–394 (2011).
2. Fairchild, P. J. The challenge of immunogenicity in the quest for induced pluripotency. Nat. Rev. Immunol. 10, 868–875 (2010).
3. Lu, X. & Zhao, T. Clinical Therapy Using iPSCs: Hopes and Challenges. Genomics Proteomics Bioinformatics 11, 294–298 (2013).
4. Kim, A. & Pyykko, I. Size matters: versatile use of PiggyBac transposons as a genetic manipulation tool. Mol. Cell. Biochem. 354, 301–309 (2011).
5. Woltjen, K. et al. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature 458, 766–770 (2009).
6. Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S. & Gregory, P. D. Genome editing with engineered zinc finger nucleases. Nat. Rev. Genet. 11, 636–646 (2010).
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