One of the fundamental techniques of modern molecular biology is immobilization of nucleic acids on a solid support and subsequent detection of specific sequence of interest. A Southern blot is a method routinely used in molecular biology labs for detection of a specific DNA sequence in DNA samples. It was named after Edwin M. Southern who developed this procedure at University of Edinburgh in 1973. Edwin M. Southern was interested in the 5S rRNA genes and was facing problem to purify it. A few years before the Southern blot was developed, Tom Kelly and Hamilton Smith, both at Johns Hopkins University, had shown that restriction endonucleases cut at specific sequence sites along DNA. Kenneth and Noreen Murray who were leading figures in molecular biology at that time, introduced Southern to these restriction endonucleases. In 1971, Kathleen Danna and Daniel Nathans, also of Johns Hopkins University, showed that DNA fragments could be separated by gel electrophoresis. Southern wanted to exploit gel electrophoresis as a way to purify restriction fragment of 5S rRNA genes. As rest...
In this experiment, we perform a gel electrophoresis on the DNA. In this process, the enzymes were run through the gel electrophoresis to determine their relative sizes for each of them. The results of the certain DNA fragments are used in the final step, which is to construct a map of the DNA molecule. If we use different enzymes to cut DNA, then not every restriction site will be cut by all of the enzymes. The objective of this lab is to perform restriction enzyme of digesting plasmid DNA and constructing a map of plasmid from the results made from the experiment. Using this technique we understand what a DNA restriction enzyme is and how it works. In this process, the enzymes were run through the gel electrophoresis to determine their relative sizes for each of them. By following the experiment, we determined the positions of the restriction
Service Corporation International (SCI) is an international company that owns and operates cemeteries, funeral homes, crematories and sells caskets, urns, and vaults. SCI is the largest owner of funeral homes and cemeteries in the United States and also operates in Germany, South America and Canada. According to Business Week the funeral or deathcare industry makes over $16 billion a year in the United States and is made up of approximately 25,000 small businesses that are sought after by large companies such as SCI, and a handful of larger corporations.
This experiment was very successful as a credible restriction map for the unknown plasmid could be constructed. Within this experiment, both single digest and double digests consisting of three restriction endonucleases were used in order to map out the restriction sites of the enzymes making up an unknown plasmid. In order to separate the DNA fragments by their distinct number of base pairs, it was necessary to run an agarose gel electrophoresis. Within the gel electrophoresis, it is necessary to run a 1kB ladder in the first well. This ladder contains numerous known lengths of base pairs, and is run next to and unknown product in order to approximate the sizes of unknown fragments simply by comparing the unknown fragments to the coinciding fragments of the known ladder. This ladder gives us the ability to precisely and accurately draw conclusions about the results derived from the gel electrophoresis as it serves as an essential reference point. Because of the known fragments in the ladder, we were able to create a standard curve. Within the standard curve, the distance the fragments traveled was plotted against the length of the known base pairs within the ladder. Once the points were plotted, a line of best fit was constructed and an equation of the line was electronically derived. By plugging in the measured distance of how far the fragments traveled, shown by “x”, into the equation for the line of best fit, the lengths of the base pairs created by the restriction enzymes was able to be calculated.
limitations” with his invention of the Polymerase Chain Reaction or “PCR” (240). As Silver describes
The model we use to explain how the cell membrane works is called a fluid mosaic model. The Fluid mosaic model was created by S.J Singer and Garth Nicolson in 1972. Between the living machinery of the inner cell and the harsh conditions of the outside world, stands the cells plasma membrane. As crucial as this barrier is, its surprisingly flexible, push it and it will move, poke hard enough and it will break and begin to regroup. We first look at the molecule called phospholipids when thinking about the plasma membrane. Lipids usually have three fatty acid chains, however this molecule has two fatty acid chains. Instead of the third fatty acid chain, a phosphate group is added on. This phosphate group is what makes the phospholipids so special. It is polar and hydrophilic, whereas the rest of the molecule is non-polar and hydrophobic. The hydrophilic heads point outwards as it attracts water molecules, whereas as the hydrophobic tails point inwards due to its mutual attraction and water repulsion. Therefore creating the phospholipid bilayer, which is the basis of all cellular membranes. Throw in some cholesterol and some carbohydrates and you have the basic structure of a plasma membrane. From chemical analysis, we conclude that there are protein in the cell membrane. Using freeze-fracture imaging and scanning electron microscope, we are able to differentiate between the extracellular surface of the membrane and the inside lipid bilayer. Within these lipid molecules, we also find different proteins, which do various things for the cell. For instance, they receive signal from the world outside, and also transport nutrients and waste. So nature composes the membrane with a composition or mosaic of different lipids, carbo...
Gel electrophoresis is used in a variety of settings, particularly in molecular biology. Besides being used to separate nucleic acids, such as DNA and RNA, gel electrophoresis is also employed to divide proteins (Gel Electrophoresis). According to research, electrophoresis is applied for the following reasons, "To get a DNA fingerprint for forensic pur...
Schizophrenia is a complicated, mostly permanent psychological disorder involving a disturbances in the relation amongst thought, emotions, and behaviour, leading to defective perception, inappropriate actions and feelings, withdrawal from reality. The National Mental Health Commission makes 10 recommendations, including reducing the use of restraint, seclusion and involuntary treatments. Recommendation 6 states, “There must be the same national commitment to safety and quality of care for mental health services as there is for general health services.”
The Scientific Method is the standardized procedure that scientists are supposed to follow when conducting experiments, in order to try to construct a reliable, consistent, and non-arbitrary representation of our surroundings. To follow the Scientific Method is to stick very tightly to a order of experimentation. First, the scientist must observe the phenomenon of interest. Next, the scientist must propose a hypothesis, or idea in which the experiments will be based around. Then, through repeated experimentation, the hypothesis can either be proven false or become a theory. If the hypothesis is proven to be false, the scientist must reformulate his or her ideas and come up with another hypothesis, and the experimentation begins again. This process is to be repeated until a theory is produced. The production of a theory is usually called the conclusion. After considerable testing of the theory, it may become what is known as a law, but laws are only formed in very rare occasions where the theory can be proven without a doubt, which is usually done through induction.
Heart rate is the different intervals of a heart beat and is influenced in the sino-atrial node of the heart by parasympathetic and sympathetic input (Meule, et al., 2012). Heart rate is influenced by a person’s physical conditions, and health (Thayer et al., 2010). Body weight, alcohol, and smoking all influence our heart rate (Thayer et al., 2010; Karason et al., 1999). Our whole lifestyle influences our heart rate. From when we wake up to when we go to sleep, each activity our day has influences whether our heart rate increases or decreases. A data collection worksheet was given to me in a class and I had to fill out a survey and a list of activities. Each student had their information entered into a spreadsheet where I had to then choose two activities. One activity has a higher heart rate than the other and that is what we need to find using the scientific method. I hypothesize that the heart rate before resting is significantly lower than the heart rate when one eats dinner. The steps used in the scientific method is materials and methods, and the results that conclude whether the hypothesis was correct or wrong.
In this inquiry the relationship between force and mass was studied. This inquiry presents a question: when mass is increased is the force required to move it at a constant velocity increased, and how large will the increase be? It is obvious that more massive objects takes more force to move but the increase will be either linear or exponential. To hypothesize this point drawing from empirical data is necessary. When pulling an object on the ground it is discovered that to drag a four-kilogram object is not four times harder than dragging a two-kilogram object. I hypothesize that increasing the mass will increase the force needed to move the mass at a constant rate, these increases will have a liner relationship.
The information gained may support or yield opposite results based on predictions being tested. My independent variable would be time and the dependent one would be the enzyme pectinase. I believe the key feature of my experimentation is the control of most factors so that the influence of a single factor can be seen clearly.
What is Science Essay Incomplete The word "science" means "knowledge." The baseline definition of "science," then, is human knowledge. Empirical thought is founded on the idea that all knowledge of the world comes from sensory experience; this sensory experience can be trusted to give us an accurate picture of the world. From sensory experience, we can derive the principles whereby the world works by observing phenomena repeatedly and in controlled circumstances.
Nature of science or NOS is a term that refers to the epistemic knowledge of science, the knowledge of constructs and values that are intrinsic to the subject. The constructs and values include historical groundwork to scientific discovery and social incorporation such as sociology, philosophy, and history of science (“Nature of Science”). Nature of science, in my opinion, should not be explicitly taught in high school science curriculum. The basis for my standing on the issue is representative of the lack of a fundamental standard understanding of what Nature of Science is, as well as the lack of effectiveness in explicitly teaching Nature of Science which I will expand on further in
Science is a method of understanding how things work. It is important because we need science in order for things to work and to develop new technology that is used in every day life. It is personally important to me because I really want to become a vet when I get older and I would need to do really well in science. Even though science isn’t exactly my best subject, I am willing to put in the hard work and determination so I may eventually get better and learn what I need to know.
Students make the same projects year in and year out because they do not have easy