neuroendocrine cervical cancer

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Neuroendocrine Cervical Cancer induced by Human Papilloma Virus (HPV) is highly aggressive and rare disease with high invasion even in its early stage. There is a lack of knowledge for the pathogenic of this cancer because there is no efficient cell line has been cultured, so the mechanism for the invasion of this cancer is ambiguous. Moreover, no plan for treatment has been approved. Therefore, we were inspired by new technique of Conditionally Reprogramming Cells (CRC), which had been developed by Dr. Schlegel and his colleagues in Pathology Department of Georgetown University. The (CRC) technology is based on using Rho kinase inhibitor Y-27632 and irradiated feeder cells to provoke indefinite proliferation of cells derived from various primary human keratinocytes (Yuan H, Myers S, Wang J, et al). Furthermore, Dr Hang Yuan an associated professor at Dr, Richard Schlegel’s laboratory of Pathology in Georgetown University have successfully isolated the first cell line from neuroendocrine cervical cancer GUMC395 cells by using a Modified Conditionally Reprogramming Cells. The original technique of CRC required digesting tissue with collagenase and trypsin. Then, culturing with J2 murine fibroblast cells and F-media containing Rho kinase inhibitor Y-27632 which, it is commonly used as a definite inhibitor of Rho-associated coiled-coil forming protein serine-threonine kinase (ROCK) family of protein kinases (Ishizaki, et al). In contrast for this experiment we only used F-media, Y-27632, and collagen-coating flasks. This technique gives us a significant propagation of GUMC395 cells.

In this laboratory of pathology scientist are interested to study Human Papilloma Virus (HPV), which cause cervical cancer. In fact, this virus associat...

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Recently, Dr, Hang Yuan has study and identifies this cancer type. After, isolating the first and the only cell line, he found that this type of cancer was HPV 16 positive, and the viral genomic was integrated in the host cell (human). Also, it was integrated in Myc gene site and both HPV and Myc are amplified and translocated. This observation indicates the high aggressiveness of this cancer. For confirmed studies, he tested these cells into soft agar assay and xenografts. Both tests show positive result, this proves that they are cancers cells. As Dr, Yuan said “this is a highly aggressive and rare cancer”. For this reason, we need to focuses our study in this cancer by propagating continuous cell line via easy CRCs technique. We believe that successful propagating of this cell line will offer more opportunities to study this type of cancer intensively.

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