EcM root-tips fungal diversity and species richness
In total we isolated 1052 root tips which gave us 590 reliable sequences. The results from diveristy statistics show that the locations were undersampled and more density of samples would be needed for the full insight. We have found a significant difference in EcM species assemblage between the two pine species (df = 1, p = 0.016, significance level < 0.05) and between locations as well (df = 4, p = 0.002, significance level < 0.01). We also tested the difference of EcMf exploration types. P. strobus preferes the contact exploration type and P. sylvestris preferes the medium type (df = 1, p = 0.007, significance level < 0.01).
The line represents the S (est) vaule computed by EstimateS program, which is a identical to MaoTau in earlier versions of the program. The tables species accumulation curves of ectomycorrhizal (ECM) fungi in study sites with increasing sample size.
The amount of ergosterol in the mesh bag samples varied between 0,0258 µg/g and 0,45 µg/g (mean 0,103 ; median 0,091 µg/g). The highest values have been found on the location Babylon which is located in the most protected area of the NP. We found a significant difference between the amount of
5.2.2 EcM fungal sporocarps biomass
The greatest difference was observed while coparing the biomass of EcM fungal sporocarps. There was almost 100% more (df = 1, p = 0.03411, significance level < 0.05) EcM sporocarp biomass in the undergrowth of P.sylvestris (see pic. 5, left). This result was almost obvious while seeing the plots on first sight, especilaly during the peak of fungal growth season. There was also a significant difference between each sites (df = 4, p = 0.02115, significance level < 0.05).
5.2.3 Saprotroph...
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...saprotroph fungal sporocarps abundance, for example allelopathic substances (Javaid & Samad, 2012), litterfall production (Ferrari, 1999) and number of other biotic and abiotic factors. For this reason, we monitored the biomass of saprotrophic fungal species sporocarps on each site as well. Despite the thick layer of leaf litter, which might provide decomposable organic matter and almost no native higher green plants in the P.strobus undergrowth, were saprotrophic fungal sporocarps relatively abundant when compared to the P.sylvestris stands (see pic. 5, right). The measured amount of saprotroph sporocarp biomass on P. strobus sites was compared to the P. sylvestris sites slightly higher, but this result had not reached any level of statistical significance. However it is a clear evidence of non-limited growth of saprotrophic fungal species on the P. strobus sites.
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