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Ph effects on enzyme activity
Introduction to effects of temperature on enzyme activity
Introduction to effects of temperature on enzyme activity
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What pH the Enzyme Named Peroxidase Works Best At The aim of my investigation is to find out what pH the enzyme named peroxidase works best at. We can find the enzyme peroxidase if we grind up celery, or most other animal and plants. A chemical reaction in plants and animals produce hydrogen peroxide, this can poison them if it is allowed to accumulate. The enzyme peroxidase acts as a catalyst for decomposing hydrogen peroxide, it is broken down into water and oxygen. The reaction is: 2H20 = 2H20 + O2 (Aq) (L) (G) I plan to measure the amount of oxygen given off by the celery at different pHs and whichever pH helps the enzyme produce the most oxygen. Before I do this I must do a preliminary plan to see what my other variables should be so that I can get the best results. I predict that the optimum pH will be around 7 because if it goes any lower or higher than this it could change the enzymes shape and/or charge properties of the substrate so that either the substrate cannot bind to the active site or it cannot undergo catalysis. Different enzymes have different optimum pHs and it is hard to say what it will be because some enzymes work best at low temperatures and others at high temperature. As you can see from the graph the two different enzymes, represented by red and green lines, have a different optimum pH. When the pH is altered is will become denatured or it may be optimum it depends on what is the best pH that the enzyme responds to. I believe it will be 7 because this is in the middle, 7 isn’t too high or too low but it could be wrong depending on the enzyme. I think it is pH7 because this is ... ... middle of paper ... ...yme more. I would also try and get the celery to be the same age because my experiments over two days with two different celeries, which could have had an effect and gave odd results. The odd results could of came from anything though, we could of measured slightly wrong, measured the solutions wrong, or when we did measure the solutions there could have been leftover acid in the syringes. I was able to get sensible results in the end after redoing the tests that had odd results. But overall I successfully carried out my investigation fairly and accurately. My experiment was good because I have measured all my solutions accurate, added the solutions together in the same order, timed my experiment to the best of my ability, I have kept the temperature the same and I have produced an experiment that is fair and accurate.
b) Comprehensive diagnostic chemistry panel with significantly increased amylase (1626 with normal being 300-1100 U/L), total
enzyme in the 2cm³ as they may have been 36 beads instead of 37. Its
The purpose of this experiment was to discover the specificity of the enzyme lactase to a spec...
a PH range of an about 5-9 and works most efficiently at neutral of PH
What Affects the Rate of Breakdown of Hydrogen Peroxide by Enzymes Aim = == The aim of this experiment is to find out how temperature and concentration affect the breakdown of hydrogen peroxide by an enzyme (yeast). I hope to achieve reliable results that will confirm my predictions.
can be split up into five numbers and I will be able to find the Maxi
From looking at the results I can conclude that when the pH was 3 and 5. No oxygen was produced, therefore no reactions were taking place. This was because the pH had a high hydrogen ion content, which caused the breaking of the ionic bonds that hold the tertiary structure of the enzyme in place of the syringe. The enzyme lost its functional shape.
There is an even trend going up, with a straight line to fit as the
Purpose: The purpose of this lab is to explore the different factors which effect enzyme activity and the rates of reaction, such as particle size and temperature.
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
I blanked it with 2 cm³ water, 1 cm³ amylase and 3 drops of iodine.
The pH of the solution would alter the rate of the reaction if it was
Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide
Investigating the Effect of the Enzyme Catalyse On Hydrogen Peroxide Introduction The aim of this experiment is to determine the effects of varying enzyme (catalyse) on Hydrogen Peroxide. Hydrogen Peroxide + Catalyse à Water + Oxygen 2H2O2 à H2O + O2 + Heat Apparatus & Diagram [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE] Bung Potato Hydrogen Peroxide Water Collected Oxygen Delivery Tube Measuring Cylinder [IMAGE] Using the Equipment Safely It is important that we use the apparatus carefully, as safety will be an issue throughout the whole experiment. We will wear goggles and an apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful, ensuring they don't come into contact with our eyes, skin or clothes. Catalyse is an enzyme found in all living cells.