What Is Isolation And Isolation Of Eugenol From Cloves

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Isolation and Characterization of Eugenol from Cloves Jean Theresse Perez Human Biology 22 De La Salle University-Dasmariñas Dasmariñas City, Cavite, Philippines ABSTRACT Eugenol was extracted from cloves through the process called steam distillation. A total of 50ml of distillate were collected with temperature monitoring for every 5.0ml of collected liquid. The distillation curve showed that the temperature of volatilization of eugenol is from 98°C to 99°C which is way below its boiling point at 2540C. Chemically active extraction was conducted in order to remove impurities particularly eugenol acetate and caryophyllene. This involves the addition of several solvents particularly hexane, NaOH, HCl, then hexane again in succession.…show more content…
The sample was subjected to steam distillation as illustrated in Figure 1. A total of 50ml of distillate was collected while recording the temperature for every 5.0 ml of distillate. The distillate was transferred into a 250ml Erlenmeyer flask and 3.0 g of NaCl was added. The flask was cooled and the content was transferred into a 250-ml separatory funnel. Then 25.0ml of hexane was added and the mixture was shaken for 5 minutes with occasional venting. The aqueous layer was discarded and the organic layer was left inside. About 25.0ml of 10% NaOH was then added and the mixture was shaken as before. The aqueous layer was collected and then cooled in an ice bath. It was then acidified with enough 6.00 M HCl while the pH is being monitored with red litmus paper. Another 25.0 ml of hexane was added and the mixture was shaken as before. The hexane extract was saved and a small amount of anhydrous sodium sulfate was added. The mixture was then swirled for a couple of minutes then filtered. A small amount of the final extracted was tested separately with 1% FeCl3 and Bayer’s reagent.…show more content…
Initially, NaCl was added in order to demulsify the distillate. Next is the addition of NaOH which solubilise the acidic eugenol into a phenoxide salt, leaving the other components that are neutral in nature. Addition of HCl to the base neutralizes the phenoxide to reform the original acidic form of eugenol. Hexane then solubilises eugenol from the aqueous layer. Anhydrous sodium sulphate was added in order to remove the remaining water in the organic
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