INTRODUCTION: It is often thought that bacteria are usually found on the surface and orifices of organisms. However, one particular genus of bacteria does not conform to this premise, Wolbachia. Wolbachia is a bacterium found within the cells of arthropods. In addition, it is estimated that Wolbachia is found in approximately 66% of all insects (Hilenboecker, 2008). Several studies have been conducted and indicate that the vast majority of arthropods are affected during the reproduction phases. One such study indicates during cell division, Wolbachia isolates itself around spindle poles, and may become incorporated into newly formed daughter cells (Hiroyuki, 1995) In order for Wolbachia to spread within a population, several factors may occur, such as male killings, feminization, and cytoplasmic incompatibility(Toon Ruang, 2004) . In this study, Photinus Pyralis is tested to determine if Wolbachia is present in the genes of these fireflies. Photinus Pyralis, commonly known as a firefly, attract one another through specific flashing signals. These light signals are in the reproductive regions of Photinus pyralis, which is a common infection site for Wolbachia to settle …show more content…
Thus, indicating that PCR was successful. CO1-cytochrome oxidase was present in the gel, however, Wolbachia was negative (Figure 1). Out of the total gels, 20% had possible positive results for the Wolbachia gene. The majority of the 20% of possible positive results for Wolbahcia where female with only one possible positive for the male Photinus pyralis. Due to the experimental error of DNA extraction, 40% of the gels were not included in the analysis. All the PCR groups were successful, due to the presence of the Wolbachia genemocis in all of the gels. However, 6 of the gels failed to extract DNA. Overall, 40% out of 100 failed to extract DNA. In addition, out of 100%, 1% had reagent
If you put the gummy bear in Vinegar it will not get bigger also if you buy it in the baking soda it will also not get bigger. The water will make the gummy bear get bigger because of the material that it made by the gummy bear.
Planarians are free-living, carnivorous flatworms found in the Phylum Platyhelminthes, Class Turbellaria. Although the Phylum Platyhelminthes is known for having the animals with the most parasitic species, the class Turbellaria which consist of the Planaria, are a non-parasitic species. Platyhelminthes which translates to "flat worm" are triploblastic animals. This means that they have three tissue layers, the endoderm, mesoderm, and ectoderm. Planaria also are monoecious organisms, meaning that they have both female and male sex organs in one organism. Another characteristic of the Planaria is that they do not have a true body cavity, meaning that they are acoelomate organisms.
When the PCR technique is completed, the tubes are stored at 4°C until analysis of the tubes. To analyze the PCR results with the gel electrophorese, 2.5ul of the 10x loading dye is added to each PCR reaction tube. The gel for the electrophorese consists of 1.5% agarose gel with 0.5x TBE and 200ng/ml ethidium. bromide. The sand is a sand.
Firstly, samples were taken out carefully. The frozen tissues of sea cucumbers were thawed in the sink with running tap water followed by multiple washes using distilled water to remove the foreign particles. The surgical blades, surgical blade-holder, and labelled sample tubes were prepared. Then, the tissue samples were cut-sliced from each samples followed by storage in the properly labelled tubes. Each sample was stored in separate tube. Different blade was used for each sample. The obtained in-tube-samples were stored in -20 oC freezer for the next step of DNA extraction.
The life cycle of Naegleria fowleri includes three different stages: amoeboid trophozoites, flagellates, and cysts; due to the transitory nature of the microbe and the specific forms that it can take, it is frequently referred to as an amoeboflagellate. Despite its abilities to take on various forms, the amoeboid trophozoite is the only infective stage of the microbe and it requires favorable environmental ...
The beet Lab experiment was tested to examine bio-membranes and the amount of betacyanin extracted from the beets. The betacyanin is a reddish color because it transmits wavelengths in red color and absorbs most other colors. The membrane is composed of a phospholipid bilayer with proteins embedded in it. The phospholipid bilayer forms a barrier that is impermeable to many substances like large hydrophilic molecules. The cells of beets are red and have large vacuoles that play a big role for the reddish pigment. This experiment aimed to answer the question, “How do cell membranes work?” The hypothesis we aim to test is: Cell membranes work as a fluid mosaic bilayer of phospholipids with many embedded proteins. We predicted that the 50% Acetone will break down the most betacyanin. Our hypothesis was proven wrong by our data collected. We could test our predictions by doing the experiment multiple times and compare the
An example of bioluminescence is a firefly. The production of light in bioluminescent animals is caused by converting chemical energy to light energy (Bioluminescence, 1 of 1). In a firefly, oxygen, luciferin, luciferase (an enzyme), and ATP combine in the light organ in a chemical reaction that creates cold light (Johnson, 42). This bright, blinking light helps the male firefly attract female fireflies as a possible mate. Other examples of bioluminescent organisms are fungi, earthworms, jellyfish, fish, and other sea creatures (Berthold Technologies, 1 of 2).
Disease and parasitism play a pervasive role in all life. Many of these diseases start with microparasites, which are characterized by their ability to reproduce directly within an individual host. They are also characterized by their small size, short duration of infection, and the production of an immune response in infected and recovered individuals. Microparasites which damage hosts in the course of their association are recognized as pathogens. The level of the interaction and the extent of the resultant damage depends on both the virulence of the pathogen, as well as the host defenses. If the pathogen can overcome the host defenses, the host will be damaged and may not survive. If on the other hand the host defenses overcome the pathogen, the microparasite may fail to establish itself within the host and die.
N fowleri has three stages of their cycle. In the amoeboid trophozoite stage, they are infectious and measure 10-35 µm long. The trophozoite transforms to a non-feeding flagellate when food sources are limited. Flagellates are motile and measure 10-20 µm in length. The amoeba or flagellate will form a cyst, the dormant stage, if the environment is too cold and not conducive to continued feeding and growth. When the organism is in the cyst stage, it has a single layered wall and only one nucleus. The cyst measures 7-1...
Polymerase Chain reaction (PCR) is used to isolate a predetermined strand of DNA on the double helix. Once the desired DNA is isolated it is able to be copied as much as needed (2). In this experiment PCR was used to isoloate Vangl2 from Zebra fish embryos. In a PCR experiment, a primer is used to find and isolate the desired nucleotide sequence of DNA (2). In this experiment two primers were used as follows:
A model which synthesizes the key factors determining parasitic virulence and can predict the entire range of evolutionary outcomes is crucial to our understanding of the history and future of species interaction.
“David M. Lodge // Department of Biological Sciences // University of Notre Dame.” University of Notre Dame. 2009. Web.1 Mar. 2010.
The purpose of this lab was to study the response of the genus Daphnia to chemical stimuli and to examine human responses to different stimuli. A stimulus is an incentive; it is the cause of a physical response. Stimuli can have a physical or chemical change; an example of a physical change is a change in temperature and sound. An example of chemical change would be changes in hormone levels and pH levels. Muscular activity or glandular secretions are responses that occurs when stimulus information effects the nervous and/or hormone system. Daphnia is a genus; it is a small crustacean that lives in fresh water. The body of the daphnia is visible and its internal organs are clearly seen thus it was chosen for this exercise. The
Wolbachia is a form of bacteria that infects different insects and stops them from spreading disease to their offspring and infecting more people with that disease. For example wolbachia is used to prevent the disease zika from spreading and what they do is that they infect mosquitoes with wolbachia and that stops zika and stops them from infecting people and spreading it. what they are hoping happens is that when mosquitoes mate they will spread wolbachia to their offspring and it will stop the spread of zika.
Engelkirk & Burton (1979) state that bacteria can reproduce asexually by simple division of cells and some bacteria reproduce sexually by conjunction. A bacterium is a waste producer of products and secretions. This allows pathogens to invade their hosts to cause disease some of these harmful diseases are Scarlet fever, an acute illness, characterized by a reddish skin rash, which is caused by systematic infection with the bacterium streptococcus. St. Anthony’s Fire is another bacterial disease. “St. Anthony’s Fire which is an acute superficial form of celluitus involving the dermal lymphatic, usually caused by infection with streptococci and chiefly characterized by a peripherally spreading hot, bright red, oedematous.