Vitex Trifolia Essay

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Vitex trifolia is found in tropical and sub-tropical regions, although vitex species may be found in temperate zones. Vitex trifolia belongs to genus vitex and family Verbenaceae there are approximately 270 known species of shrubs and trees[17]. Vitex trifolia posses larvicidal, wound healing, anti-HIV, trypanocidal, anticancer activity[10]. It is also used as a anti-bacterial, anti-inflammatory, antipyretic agent. They are also used as sedative for rheumatism, headache and common cold in some countries. Vitex species used in traditional medicine to treat ailments like wounds, allergies, asthma and body pains[15].

Colletion of plant materials
The fresh leaves of vitex trifolia were collected from well grown trees in Tamilnadu Agriculture University, Katuthotam, Thanjavur district, Tamilnadu. The fresh leaves were washed twice in ordinary water and in distilled water. The washed leaves were shade dried in room temperature for 15 to 20 days.

Preparation of extracts from leaves of vitex trifolia
Selected organic solvents were used for the extraction of phytochemical compounds from leaf materials of vitex trifolia. The shade dried leaves are finely powdered using blender[12]. 15gm of the powdered leaf were first extracted with the solvent petroleum ether for defatting the extract using soxhlet apparatus. The defatted powdered were dried and extractions were carried out with the solvents petroleum ether benzene, acetone, ethanol and distilled water using soxhlet apparatus successfully[2]. Vaccum rotatory evapourator was used to evapourate solvents and the concentrated extracts were obtained. The concentrated extracts were subjected to qualitative and quantitative test for the identification of phytochemicals present in the leaf...

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...roids, tannins(0.521mg) and terpinoids(0.321mg). Ethanol extract showed the presence of alkaloids, flavonoids(0.232mg), phenols(0.163mg), saponins(0.123mg), tannins(0.461mg) and terpenoids(0.361mg). Finally water extract showed the presence of flavonoids(0.423mg) and tannins(0.62mg).
The antibacterial activity of the different extracts was done by disc diffusion method. The extracts have shown different degrees of antibacterial activity. In Bacillus subtilis the acetone extract of V trifolia have shown the zone of inhibition at 0.5cm, the ethanol extract of V.trifolia have shown the zone of inhibition at 0.75cm.
In Escherichia coli the acetone extract of V.trifolia showed the zone of inhibition at 0.15cm, the ethanol extract of V.trifolia have shown the zone of inhibition at 0.25cm, the Dis.H2O extract of V.trifolia have shown the zone of inhibition at 0.35cm.

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