Weigh the testing assembly (M1).
Remove the stopper and tube assembly and pour 650 mL ± 50 mL of cold water (below 27°C) into the flask. Replace the stopper and tube assembly, weigh the flask and record the mass (M2).
Introduce a temperature sensor through the shorter of the two pipes into the stopper and into the center of the water column in the flask. Agitate the flask and note the temperature of the water in the flask (T0). Continue agitating the flask until the temperature remains constant.
Dryness Test (Continued)
Ensure the reactor is empty.
Select the appropriate SIP cycle.
When the steam supply valve to the reactor first opens, connect the rubber fitting to the
Sampling tube. Record the steam temperature for the duration of the test
2. Drop a gummy bear into each of your prepared beaker or cup and place the beaker or cup
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the mass and initial temperature of the water. Next, impale the food sample on the needle. Next, light
4. Pour hot water into one beaker and adjust the temperature to 39°C by adding colder water if needed
I am going to carry out an experiment to measure the change in mass of
Check if there are any leaks especially around the stopcock, the valve at the bottom of the buret. When it comes to calibration, it is calibrated by transferring water into an Erlenmeyer flask and weighing it using an analytical balance. This is done for two (2) trials, each with varying amounts of water. Erlenmeyer’s mass is pre-recorded before transferring water from the buret. The volume delivered by the buret is considered the apparent, which means evident, volume. Before starting another set, the flask should be clean and dry to avoid any error. The water inside the flask will add the volume to be transferred thus making the mass of water higher than what it should really be. The mass of water obtained thru weighing by difference would then be multiplied to the buoyancy of water at the recorded temperature which will then be the corrected mass of the water. The corrected mass of the water is then divided by the density of the water, at the same temperature, and will be considered as the true mass. Correction value can be obtained by solving for the difference between the true volume and the apparent volume. Some of the correction value computed are negative and it only means that there is an error that occurred. One of the possible errors in most volumetric glassware’s is reading the wrong volume. Volume must be read based on the lower meniscus at eye
* Heat the end of the glass tube so as to close it. The tubeÂ’s end
filled a water bath with warm water at a temp of 35oC and placed the
After the water, has been boiling for 10 minutes, and the temperature inside the test tube has been stable for 5 minutes, record the temperature and remove the thermometer.
Start with the hot water and first measure the temperature. Record it. 8. Then pour 40 ml into the beaker. You can measure how much water was used by looking at the meniscus.
Then add growth media until the sample is submerged beneath the 2-3 ml of liquid, cap the tube and invert several times to mix thoroughly, incubate the tube while shaking vigorously in a shaking incubator at 250 rpm for a couple hours, then allow the sample to sit.
5.) One at a time, place your test tubes in the water bath and heat the first test tube to 25 , the second to 50 , the third to 75, and the last to 100 degrees c. Remeber to stir with your stirring rod every so often.
In a 100ml beaker place 50mls of water, measure the temperature of the water and record this initial temperature onto a table. Set the timer and add one teaspoon of Ammonium Nitrate to the water, stir this continuously until the Ammonium Nitrate has dissolved.
2. In the large beaker, put water and boil it completely. After that, remove the beaker from heat. 3. Sample tubes (A-D) should be labeled and capped tightly.
Remove the extra solvent on a steam bath under a hood while flushing the flask with N2 gas, leaving the crude extract. Weigh extract.