Membrane Damage For the lab experiment for Membrane Damage, we tested the extract pigment and diluted it. When the pH solutions are added, this will cause it to be in a range of absorbance. We used materials as follows. Obtaining a beet we proceeded to cut small individual cubes. We then rinse each cube to remove any damaged pigments with deionized water.
The first chemical to stain the slide is crystal violet which contains a purple color stain and indicates a gram positive bacteria. Add enough crystal violet stain to the slide and let it sit for sixty seconds. Then, rinse the substance under slow running water. The second chemical to stain is gram iodine which fixes the dye to the cell walls of bacteria and makes the stain permanent. Add the same amount as preview of the gram iodine stain to the slide.
Records of how large of a circle the antibiotics made around the bacteria were put into the Table. After completing the data, each plate was to be put in a tray for autoclave sterilization. The most important thing that was to be remembered was to wash hands, and everything that touched the dishes.
Introduction The objective of this lab was to identify unknown bacteria culture by using various differential tests. There are many reasons for knowing the identity of microorganisms including to find the correct antibiotic to treat infections the bacteria may have caused. All the methods and techniques used to identify unknown bacterium #79 was learned in the microbiology laboratory. Test Result Unknown# 79 was grown on TSA slant in a 370 c temperature after two days, the microbe replicated to more than 100 microorganisms, which has cream pigments, and convex elevations. The microbe entire margins and convex elevations.
There were three agar plates; one was incubated at room temperature, the second at 30 degrees Celsius, and the third at 37 degrees Celsius. By placing each plate at a different temperature optimal growth temperature can be predicted for both species of bacteria. Week 1, Day 2 (12 November 2000) After 48 hours of incubation the agar plates were viewed. Individual colonies were tested for successful isolation by gram staining and then viewing the stained bacteria under a microscope. Isolation was successful.
There was a variety of testing performed on the sample that was obtained to help to identify what kind of bacteria there might be. The bacteria were first transferred from the nutrient agar plate to another plate using aseptic technique. To begin the testing of the bacteria, a gram stain test was performed to determine if the bacteria was gram positive or gram negative, where a smear was made into a slide and examined under a microscope. After examination of the slide, it was determined that the results of the sample were gram positive as the area appeared purple. By viewing, the cell had a large rod shape to it.
The next day, we will inoculate another tube with 4.5 mL of water sample that we take from poultry farm nearby that we suspect as source of Salmonella contamination on the squid water farm. We also add 0.5 mL of overnight Salmonella culture and 0.5 mL of 10X tryptic soy broth. The mixture will be incubate for about 24 to 48 hours at 37oC. During this incubation period, we expect phage in water sample will be able to bind to Salmonella. The phage also will replicate and lyse the bacteria.
These controls were given their own area on the media and streak plated. The controls consisted of the live ampicillin sensitive strain, the dead ampicillin resistant strain and the live ampicillin resistant strain plated onto each medium. Aseptic technique was used in treating all solutions, media and transfers between. (Part 2) In the second set of experiments 1.0µL of kanamycin sensitive bacteria was added to two test tubes, both subjected to the same treatment. The bacteria in the... ... middle of paper ... ...colonies for live ampicillin sensitive cells grown on LB Amp100 media with only the buffer.
Paper discs containing different types of antibiotics then placed on the hardened agar plates. The inhibition zones of the bacteria on agar plates then is observed after 24 hours to determine the effectiveness of different types of antibiotics. The main result of this experiment is that the paper dics soaked with tetracycline has the greatest zone of inhibition in both Bacillus subtilis and E. Coli agar plates. Therefore, from this experiment, we can conclude that Tetracycline is the most effective antibiotics for Bacillus subtilis and E. Coli. Introduction: Antibiotics Antibiotics are among the frequently prescribed medicine for treating bacterial infection.
The identification of the bacterial unknown was determined through a series of tests using differential media and a gram stain. These tests revealed information about the motility, the metabolism, and the enzymes of the unknown microorganism. The most basic technique for all tests is called the aseptic technique. This technique is “to prevent contamination of the sample” (Leboffe and Pierce, 2010). This is the first technique taught to students in the lab.