Wait a second!
More handpicked essays just for you.
More handpicked essays just for you.
Periodontal disease note
Periodontal disease case study
Periodontal disease case study
Don’t take our word for it - see why 10 million students trust us with their essay needs.
Recommended: Periodontal disease note
How branded and unbranded mouthwash effect cell numbers?
Research and Rationale:
In 2001 it was reported by BBC news that serious dental diseases could be one cause of diabetes. Gingivitis is the periodontal disease that is talked about in the article, this is a disease that causes inflammation to the gums and if left untreated could lead to periodontitis which eventually leads to the loss of teeth. There is no hard evidence for gum disease leading to diabetes however a spokeswomen for the British Dental Association (BDA) said “Studies are currently underway to explore further the relationship between periodontal disease and diabetes.” She also said that there was growing evidence linking periodontal diseases with other medical conditions as well as
…show more content…
The scientist named Hans Christian Gram devised a method in 1884 to differentiate between the two different forms of bacteria relating to structural differences in their cell wall.
http://micro.digitalproteus.com/morphology2.php
On the left is the gram positive bacteria which has a thicker peptidoglycan layer. This is the ayer that retains a crystal violet dye when added to it whereas gram-negative has a thinner layer of this and so unable to retain the dye and is coloured red/pink.
Gram-positive bacteria has a single membrane surrounded by a thick peptidoglycan layer whereas Gram-negative bacteria has a thin peptidoglycan layer in between two membranes and because of this, gram-negative bacteria is more resistant against antibodies due to their impenetrable cell wall. http://www.diffen.com/difference/Gram-negative_Bacteria_vs_Gram-positive_Bacteria Mouthwash for many people is something that is used everyday, gram-negative bacteria are very strong bacteria and are hard to kill with such structural properties and from investigating this I am able to see what form of mouthwash is better for the human mouth on killing such bacteria.
The first day an unknown sample was assigned to each group of students. The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The morphology of the two types of bacteria was viewed under the microscope and recorded. Then the sample was put on agar plates using the quadrant streak method for isolation. There were three agar plates; one was incubated at room temperature, the second at 30 degrees Celsius, and the third at 37 degrees Celsius. By placing each plate at a different temperature optimal growth temperature can be predicted for both species of bacteria.
The Gram positive bacteria has been nicknamed Posi. The Gram positive species’ morphology includes having an opaque opacity with a smooth margin. The moisture content of the Gram positive species is shiny and the pigmentation is gold. The Gram positive species grows at an optimal temperature of 37°C. The shape of the Gram positive species is a cocci, with an arrangement of grapelike clusters. The Gram positive species’ size ranges from .5-1.5 µm. Oxygen requirement of the Gram positive species is facultative, and has complete lysis of red blood cells. All results are summarized in Table
The purpose of this study is to identify an unknown bacterium from a mixed culture, by conducting different biochemical tests. Bacteria are an integral part of our ecosystem. They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species. These biochemical tests are process of elimination that relies on the bacteria’s ability to breakdown certain kinds of food sources, their respiratory abilities and other biochemical conditions found in nature.
Phenotypic methods of classifying microorganisms describe the diversity of bacterial species by naming and grouping organisms based on similarities. The differences between Bacteria, Archaea and Eukaryotes are basic. Bacteria can function and reproduce as single cells but often combine into multicellular colonies. Bacteria are also surrounded by a cell wall. Archaea differ from bacteria in their genetics and biochemistry. Their cell membranes are made with different material than bacteria. Just like bacteria, archaea are also single cell and are surrounded by a cell wall. Eukaryotes, unlike bacteria and archaea, contain a nucleus. And like bacteria and archaea, eukaryotes have a cell wall. The Gram stain is a system used to characterize bacteria based on the structural characteristics of their cell walls. A Gram-positive cell will stain purple if cell walls are thick and a Gram-negative cell wall appears pink. Most bacteria can be classified as belonging to one of four groups (Gram-positive cocci, Gram-positive bacilli, Gram-negative cocci, and Gram-negative bacilli) (Phenotypic analysis. (n.d.).
Periodontal disease is the inflammation of the structures that support the teeth. This disease is the primary loss of teeth in dogs. It is caused by a build-up of dental plaque on the surfaces of the teeth and around the gums. Bacteria can accumulate in the dental plaque and irritate the gum tissue which leads to the infection of the bone that surrounds the teeth. Some effects of the disease include: bad breath, bleeding gums, oral pain, dropping food from mouth while eating and loss of appetite. In severe cases the teeth may become loose and fall out.
According to the American Dental Association, gum disease, also referred to as periodontal disease, occurs when the tissues that support and surround your teeth become infected. Many people are unaware they even have gum disease, because it isn’t a painful disease. Periodontal disease is caused by a film of sticky bacteria called plaque forming on the teeth.
P&G was founded in 1837 by William Procter and James Gamble as a maker of soaps and candles. P&G was known in Corporate America as a company to be admired and imitated. In addition, it was envied for its profitability as well as strong brand name. P&G has a long standing reputation as having life long employees. This dedication and loyalty by P&G's employees created the notion that outside sources were unwelcome and all products and ideas must come from within, however, this is not the way of the future.
Jost, Kenneth. “Diabetes Epidemic: why is this serious disease on the increase?” The CQ Researcher (March 9, 2001): 185-200
I identified the genus and species of an unknown bacterial culture, #16, and I applied the following knowledge of morphologic, cultural and metabolic characteristics of the unknown microorganism according to the laboratory manual as well as my class notes and power point print outs. I was given an incubated agar slant labeled #16 and a rack of different tests to either examine or perform myself; the tests are as follows: Gram Stain; Nutrient Gelatin Test; Carbohydrate Fermentation; Dextrose, Lactose and Sucrose; IMVIC tests; Citrate, Indole, Mythel-Red and Vogues Proskauer test; as well as a Urease and TSI Test.
Founded in 1886 by introducing medicinal plasters and antiseptic surgical dressings, Johnson & Johnson has grown to be one of the leading health care products company in the world. In its extensive history of over 125 years Johnson & Johnson’s product mix vary from pharmaceutical, personal care products, medical devices and diagnostics with the largest being pharmaceuticals.
Periodontal disease is an infection of the gingiva and alveolar bone. Periodontal disease increases in prevalence and severity as people age. Periodontal disease is precursored by gingivitis.
Magliano, DJ, Shaw, JE, Shortreed, SM, Nusselder, WJ, Liew, D, Barr, EL, Zimmet, PZ & Peeters, A 2008, ‘Lifetime risk and projected population prevalence of diabetes’, Diabetologia, vol.51, pp.2179-2186, viewed 15th May 2011.
Twenty years of research has firmly established that periodontal disease and cardiovascular disease are associated. However the exact relationship between the two is still controversial. In order to understand the relationship between periodontal disease and cardiovascular disease people need to understand the physiology, and microbiology behind both of the diseases.
The Campylobacter species observed in 1886 from Theodor Escherich in the colonic mucus of infants who had died of “cholera infantum,” but they could not be cultured. (Miliotis & Bier 2003) Mc Fadyean and Stockman in 1909 first isolated Campylobacter fetus from aborted sheep fetuses. (Miliotis & Bier 2003) After that observed that the Campylobacter which called (Vibrio fetusovid), caused septic abortion in cattle. (Miliotis & Bier 2003) This pathogen bacterium starts to create problems dysentery in the cattle.( Miliotis & Bier 2003) In 1957 the King examined people which have bloody diarrhea the reason for the disease is the Campylobacter species. (Miliotis & Bier 2003)The species of Campylobacter are Campylobacter jejuni, Campylobacter coli, Campylobacter lari and Campylobacter fetus. (Miliotis & Bier 2003) The campyloCbacter is Gram-negative thin; (Siegrist 2014) Gram-negative bacteria are bacteria that do not retain the crystal violet dye in the Gram stain protocol. (Miliotis & Bier 2003) Gram-negative bacteria will thus appear red or pink following a Gram stain procedure due to the effects of the counter stain. (Miliotis & Bier 2003) The shape has the Campylobacter is curved and motile rod like S or spiral. (Siegrist 2014) Finally the Campylobacter has single polar flagella at one or both ends and they exhibit a rapid darting motion (Siegrist 2014), like picture1.
The infection is now destroying the bone that supports your teeth. Eliminating bacteria through good oral hygiene is not possible.