The hypothesis stated, If C.elegans that express Aβ1-42, are grown in media containing large concentrations of glucose, there will be an increase in the rate in which the animals undergo paralysis, in other words there will be an increase in Aβ toxicity in the animals, because of a high exposure of glucose. Overall, the hypothesis was supported, as there was a significant increase in the worms that underwent paralysis who were grown on high glucose plates.
N2
The N2 strain did not express Aβ1-42, which means that the worms would not be able to undergo paralysis since they did not have the phenotype for it. For both the control and experimental plates, only 1 worm from each was still. Meaning the glucose had no effect on the N2 strain, as expected.
GMC101
It was found from the first paralysis assay done on the strain GM101, control plates, was that 95% of the worms were paralyzed, and by the second assay 97% of the worms were paralyzed. In other words only 1 worm was moving by the end of the assay for control plates. Overall, there was already a large amount of animals undergoing paralysis on the control, non-glucose plates. The results from the paralysis assays done on the strain, experimental plates, were similar. From the first assay, 94% were paralyzed and by the second assay 100% were paralyzed. This strain
…show more content…
Mice are usually used for Alzheimer’s research, however they are costly and it can weeks to months to get any type of significant data. By taking an experiment that was done in mice and replicating it in C.elegans, could show how useful this model organism could be to finding a cure for Alzheimer’s. The hypothesis was supported, specifically for the strain Cl2122, and there was an increase in Aβ toxicity in the worms for those grown on high glucose plates. Overall, C.elegans can be more widely used as a model organism for Alzheimer’s
improved in any way unless another sugar was utilized. There were some things that were difficult to keep constant in the experiment and this is where my results may have wavered slightly. It was difficult to keep the temperature of the warm water constant as it dipped at times which could have had an effect on how efficient the enzymes were. The delivery tubes were becoming blocked sometimes and by shaking the test tube it cleared them. However as we shook the test tube a large number of bubbles were formed which may not have formed if we didn't shake the test tube.
The meningeal worm is also known as the brain worm, or the deer worm. It frequently infects llamas and alpacas, but can infect other species as well like moose, elk, goats, and sheep. The meningeal worm is an internal parasite that is a part of the protostrongylidae family with the definitive host being the common white tailed deer. Even though they are the host, adult meningeal worms rarely cause clinical signs of disease in the deer. The white tail deer’s tolerance to infection from these worms is actually beneficial to the parasite because the white tail deer are the only species that will allow the worm to complete their life cycle. So alpacas and llamas are unsuitable hosts and that is why the meningeal worm is such a threat to them. It can cause varying degrees of damage to their central nervous system. “The cerebrospinal fluid tap is especially useful for diagnosing meningeal worm and ruling out other diseases.” (Whitehead, Bedenice 2009.) Ruling out other diseases helps to narrow down what could be ...
There is a wealth of evidence showing that animal “models” are not accurate and cannot be relied upon for safety testing and disease research. Scientists and doctors recognize that while animals are biologically very similar to human beings, they are not identical.
Lou Gehrig's disease is often referred to as Amyotrophic lateral sclerosis (ALS), this is a progressive neurodegenerative disease that affects nerve cells in the brain and the spinal cord. Motor neurons come from the brain to the spinal cord and from the spinal cord to the muscles throughout the entire body. The progressive degeneration of the motor neurons in ALS would eventually leads to their death. When the motor neurons die, the ability of the brain to initiate and control muscle movement is also lost. With voluntary muscle action progressively affected, for this reason patients in the later stages of the disease may become totally paralyzed (Choi, 1988).
Alzheimer’s disease is the most common cause of dementia. The very first stages of Alzheimer’ include symptoms like confusion and using the right words while talking. As it gets on to later stages symptoms like loss of memory and mood swings start to appear (Begley et al., 2001). Any sort of stem cell treatment is not available to Alzheimer’s patients yet, as researchers are still conducting new experiments. There are multiple neurons that get damaged by Alzheimer’s, which makes the problem even more complex and a different approach needs to be followed every time a clinical trial is conducted.
This disgusting worm parasite is spread by flies and mosquitoes. The adult worm spreads its larvae throughout the host’s lymphatic system and causes the lymph nodes to become clogged up. This also makes the tissue in the host’s body to swell up and create massive muscle deformations, otherwise known as elephantiasis. The elephantiasis mainly affects the legs and genitals. The disease also affects the eyes but that can be easily detected through close inspection but it commonly causes river blindness in the host. It’s been estimated that the parasite is one of the leading causes of blindness throughout the world.
Alzheimer’s disease is a complex illness that affects the brain tissue directly and undergoes gradual memory and behavioral changes which makes it difficult to diagnose. It is known to be the most common form of dementia and is irreversible. Over four million older Americans have Alzheimer’s, and that number is expected to triple in the next twenty years as more people live into their eighties and nineties. (Johnson, 1989). There is still no cure for Alzheimer’s but throughout the past few years a lot of progress has been made.
From these experiment many questions and future studies are brought to the table. Some questions about the experiment may include the reliability of the test suspects. Due to the different lifestyles and conditions of each test subject it could have and affect on the results of the experiment. Also the article states that there could have been a mistake in the values of PD__AMP. A future study similar to this may be hookworm’s effect on other disease like issues in the immune system. It would relate to the past two studies because in both of the previously done studies it tested the hookworm’s effect on issues with the immune system. The future studies could be improved by making the test suspects in the most similar conditions and lifestyles as possible. Also they could use a larger number of test subjects to have more accurate results.
Animals have always held a very special place in the hearts of the human race. They are our best friends, our stress relievers, members of our families, and our test subjects for experimentation. For hundreds of years, animals have been used in laboratory settings as a replacement for humans when studying the effects of medical treatments. On average, nearly one hundred million animals are used in clinical trials every year (Ferdowsian). These animals have contributed to hundreds of breakthroughs in the medical field including countless toxicity tests to determine drug toxicity to humans, and exposure to paralyzing anesthetics to create anesthesia used in surgical procedures today. These animals have been vital
Alzheimer’s is a terrible disease, the diagnosis of which marks a long and painful journey through neurofibrillary degeneration. Unfortunately, there are so many factors that lead to and expedite the disease that synthesizing a cure is no simple task by any means. Whether the cause of the disease is hyperphosphorylation of tau or beta-amyloid plaques, current medical technology can only delay the symptoms. Hopefully the future of medical research will yield a method for reversing the progression of the neurological degeneration because far too many victims and their friends and family are forced to embark on the long and painful journey of Alzheimer’s.
Alzheimer's disease is the most common form of dementia, and this terminal, progressive brain disorder has no known cause or cure. Its greatest known risk factor is increasing age which is why is it is infamous for developing in the elderly, typically in ages 65 or over, however for the 5%(1) that develop Alzheimer’s in their 40s or 50s it is known as early Alzheimer’s. Because Alzheimer’s worsens over time, those with it tend to struggle with completing daily tasks especially elderly people. Given that there is no cure for Alzheimer’s, the treatments available slow the worsening of dementia symptoms and improve quality of life for those with Alzheimer’s and their caregivers. It is not known what causes Alzheimer's, however, those with Alzheimer's have been found to have abnormal amounts of protein (amyloid plaques) and fibres.(The amyloid plaques and fibres are found in regions of the brain where problem solving and thinking take place e.g The cerebrum.) Due to the unusual amounts of amyloid plaques and fibres, it reduces the effectiveness of healthy neurons and eventually, destroying them.
Thesis/Preview Statement – Alzheimer’s disease (AD) causes a decline in brain function, it destroys healthy nerve cells. Today, we have discussed Causes, Symptoms, and Diagnosis of AD.
The mixture for that table’s flask was 15 mL Sucrose, 10 mL of RO water and 10 mL of Yeast, which the flask was then placed in an incubator at 37 degrees Celsius. In my hypothesis for comparison #4 the measurements would go up again with every 15 min. intervals because of the high tempeture and also be higher that then Controlled Table’s measurements. Hypothesis was right for the first part but was wrong for the second part of the comparison, the measurements did increase in the table’s personal flask but the measurements did not get higher than the Controlled Table’s measurements, see chart below. In conclusion, I feel that the substitution of glucose for sucrose made the enzymes work just as hard as the Controlled Table’s flask but just not as much because sucrose was too strong for the enzymes to
Alzheimer’s Disease is named after a German doctor, who specializes in the brain and nervous system, named Alois Alzheimer. This Disease forms in the brain. Alzheimer’s is the most common form of Dementia, a general term for memory loss and other intellectual abilities serious enough to enter. The Tau protein ensures the tubes in your brain stay straight allowing molecules to pass through freely. In Alzheimer’s Disease the protein collapses into strands or tangles, making the tubes disintegrate. There is visible differences of brain tissue in the from misfolded proteins called plaques and tangles. Beta-Amyloid clumps block signals and communication between cells in the brain. Researchers agree that Alzheimer’s Disease is m...