Membrane Damage For the lab experiment for Membrane Damage, we tested the extract pigment and diluted it. When the pH solutions are added, this will cause it to be in a range of absorbance. We used materials as follows. Obtaining a beet we proceeded to cut small individual cubes. We then rinse each cube to remove any damaged pigments with deionized water.
Remove approximately 0.35 g into a 250 mL beaker. check the balance and record the mass of the remaining mixture in the vial. In the laboratory hood, dissolve the copper with ~ 3 mL of nitric acid. Allow the beaker to remain under the hood until the fumes cease. The remaining solution should be blue.
Relax and is soothing purification bath for about 20 min. and then pat yourself dry. 4. The Clorox Bath Clorox bleachThe Clorox Bath is a purification bath that is one of the best ways to eliminate any heavy metals you may have inside your body. To perform this heavy metal detoxification you simply need to add 1/2 cup of Clorox bleach (make sure you only use Clorox bleach) to a tub of warm water.
After the calibration process, few drops of each solution were transferred from each test tube without removing the test tubes from their temperatures. These drops were added into the first row of wells on the spot plate, then add two drops of iodine to the wells on the spot plate and wait 1 minute. Use a color- coding scheme to convert the data to qualitative data into quantitative data this will serve as the control. Add 0.5 ml of amylase to the appropriate test tube containing starch and wait two minutes. Then add two drops of the starch-amylase mixture from each tube to the third row of
Then, using a bottle of water I placed sterile drop of water on the slide and used an inoculating loop, flame sterilized, I took a small sample of the unknown growth in my agar slant and smeared it onto the slide in a dime sized circle and then heat fixed it for ten minutes. After ten minutes had passed, I collected the ingredients needed to perform a gram stain. I got the primary stain, crystal violet, and flooded my smear for sixty seconds, and then rinsed the color off with water until the water ran clear. I then flooded the smear with the mordant, grams iodine, and let that sit on the slide for sixty seconds as well. I then rinsed the grams iodine off with water and applied alcohol to the smear to decolorize the cells; however I made sure not to over decolorize and only put enough drops on the smear till the purple ran clear.
In Gram positive bacteria, the ethanol is unable to successfully wash out the purple color because of the thick cell walls that these bacteria possess. When performing this stain on Gram negative bacteria, the ethanol will be successful and the final counterstain will result in a pink color. First, a catalase test was performed by placing the specimen on a microscope slide. 3% hydrogen peroxide was then added to the sample on the slide, and an immediate reaction was observed. During a citrate test, the bacterium was inoculated onto Simmon’s citrate slants, which were then incubated at 35 C for approximately 24-48 hours.
The first test we did was the initial Gram’s stain. This test gave detailed information about the cell wall and the structure of the growing bacteria. This was a time consuming process that involved placing bacteria onto a slide, heat fixing it, and adding different compounds to the slide. I examined it under the microscope and a purple hue with multiple cells was visualized. After the staining process, my bacteria were found to be Gram positive that had a rod shape.
A gram stain is a differential type of stain that determines whether a cell is gram-positive or gram-negative. "Bacterial size, morphology and arrangement can also be determined using this stain" (2). The negative charge on bacterial cells attract the positive charge on the stains used, thus causes the cell to be colorized (2). The gram stain conducted on unknown #53 did not retain the crystal violet when decolorized. The unknown bacteria adhered better to the Safranin stain and was shown to be a pink stained bacterium under the microscope.
• Sufficient boiling water is poured into the bowl to cover the silver being cleaned has contact with the polivit. • A chemical reaction takes place between the polivit, soda, boiling water and silver which causes the tarnish to be lifted. • After 2-4 minutes, silver should be removed from the bowl and placed into the 2nd bowl of boiling and then rinsed. • On removal from the second bowl the silver is allowed to drain and then polished with a clean cloth and then dried with a tea cloth. Source: https://www.google.co.in/search?q=Polivit+Method+cleaning&espv=2&biw=1366&bih=662&source=lnms&tbm=isch&sa=X&ved IV.
Measure out 0.1 g. of the H2TTP made previously into a 100 mL ... ... middle of paper ... ...aporates off the gel leaving open sites where polar molecules can bond. When you let silica gel out in the open, it will collect water molecules on it. Compounds would elute faster down a column that has been heated at 150 degrees for 8 hours because the gel would be dry. 3. A mixture of cis and trans isomers of the neutral complex Cr(CO)4[P(C6H5]2 is loaded onto a silica gel and eluted with CHCl3.