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Both sides of the use of DNA in criminal investigations issue
Use of DNA in criminal investigations
Both sides of the use of DNA in criminal investigations issue
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Summary
Accurate gender attribution has always been a priority in forensic casework and has always concerned the archeologists. Characteristics of forensic DNA samples analysis are similar to that of ancient DNA, small quantities of workable molecules being the main resemblance. The most frequently used markers for molecular sex attribution are the single copy gene for amelogenin located on X (AMELX) and Y (AMELY) chromosomes. Worldwide used sex determination kits were designed based on amelogenin genes amplification, especially useful in forensic casework. Sometimes these sex tests fail due to allelic dropout. New molecular markers for sex identification are constantly developed to overcome this problem. Another issue characteristic for ancient DNA studies is the contamination of samples with modern molecules of DNA. The accuracy of sex tests for ancient DNA studies depends on the possibility to discriminate between authentic ancient DNA and modern contaminant DNA.
Keywords: physical anthropology, sex attribution, molecular sex attribution, AMELX, AMELY, ancient DNA.
Introduction
Correct sex identification has always been preoccupying archeologists or anthropologists and has major importance in forensic casework for nowadays population. In past populations, the status of man and woman significantly varied and the dynamics of relations between sexes changed during time. Physical anthropology may work out identifying with a higher degree of certainty the gender of skeletal remains by analyzing a few markers that indicate the sex.
The most common physical markers are the skull and the pelvis. Skull features tend to be more robust in the man than the female and the sub pubic region presents important differences due to the ...
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The samples of DNA were obtained by plucking individual hairs from students' heads and using the PCR device to replicate. the DNA from the roots of the hair. The replicated DNA samples were then placed into the electrophoresis gel and the device was turned on. Using the methods discussed above we found that three of the fourteen samples, 21%, were homozygous and the remaining eleven samples. 79% were heterozygous in their ancestors.
Miller, Kenneth R. and Joseph S. Levine. “Chapter 12: DNA and RNA.” Biology. Upper Saddle River: Pearson Education, Inc., 2002. Print.
. DNA can be left or collected from the hair, saliva, blood, mucus, semen, urine, fecal matter, and even the bones. DNA analysis has been the most recent technique employed by the forensic science community to identify a suspect or victim since the use of fingerprinting. Moreover, since the introduction of this new technique, there has been a large number of individuals released or convicted of crimes based on DNA left at the crime scene. DNA is the abbreviation for deoxyribonucleic acid.
Both of these methods can help determine and eliminate genetic disorders, as certain conditions can be screened out using these technologies. As discussed in Meseguer et al., more than 300 diseases have been related to sexual chromosomes till date. Case studies also argue the important role of these methods in family balancing for varied family dynamics who aim to compensate their family with a representation of the other sex (Meseguer et al., 2002). Critics have also mentioned that with an increase in demand, these methods could become more economical and widely available, including to couples who may need it due to medical
contains mitochondrial DNA (mtDNA), completely intact. Mitochondrial DNA is tougher than the DNA found in cell nuclei; it is also found in the cytoplasm of a fertilized egg and is passed only through maternal lineage. This makes it much easier for the team to study and makes testing more accurate. Paabo’s team, from Leipzig, Germany, used a method of amino acid content as a way of measuring extractible DNA from the bones. The amino acid method was a means for testing the DNA content in the fossils.... ...
Forensic genetics has other applications . The " fingerprint " DNA represents a valuable tool for forensic science . As is the case with an ordinary fingerprint genetic fingerprint is unique to each individual (except identical twins ) . The determination involves the observation of specific DNA sequences which can be obtained from extremely small tissue samples , hair, blood or eventually left at the scene . As Fifty microliters of blood, semen or five microliters of ten roots of hairs are enough , and nozzles secretions and cells from the fetus . In addition to its use in the capture of criminals , especially rapists , the genetic fingerprints can be used to establish family relationships . People involved in the conservation of species use them to be sure that captive breeding is among individuals who do not belong to the same family .
Once a crime has been committed the most important item to recover is any type of evidence left at the scene. If the suspect left any Deoxyribonucleic acid (DNA) at the crime scene, he could then be linked to the crime and eventually charged. A suspect’s DNA can be recovered if the suspect leaves a sample of his or her DNA at the crime scene. However, this method was not always used to track down a suspect. Not too long ago, detectives used to use bite marks, blood stain detection, blood grouping as the primary tool to identify a suspect. DNA can be left or collected from the hair, saliva, blood, mucus, semen, urine, fecal matter, and even the bones. DNA analysis has been the most recent technique employed by the forensic science community to identify a suspect or victim since the use of fingerprinting. Moreover, since the introduction of this new technique it has been a la...
However, many of our questions still remain. The purpose of this paper is to discuss the physical aspect of gender differences in humans, otherwise known as sexual dimorphism, it’s evolutionary history in our species, and some behavioral and societal trends that are associated with it. To accomplish this, I will begin by outlining the anatomical structures that are commonly used in measuring sexual dimorphism in our species. After establishing these criteria, I will expand upon the evolutionary history of sexual dimorphism in humans beginning with the anthropoids in the Oligocene and ending with present day trends.
One particular grave, 6-0099, contained the skeletons of four individuals a male, female and two children.2 Using DNA testing they were able to determine the grave was of a father, mother and two young boys. The father is believed to be forty- to sixty-years-old, the mother thirty-five- to fifty-years-old, one boy four- to five-years-old...
"Polymerase Chain Reaction (PCR) Fact Sheet." National Human Genome Research Institute. 10 Dec. 2007. National Institutes of Health. .
...he case of forensics. You can go beyond the normal reality of blood spatter (?.. how does or where did blood spatter come into play here). The species of a set of bones can be determined through forensics anthropology. The sex of a skeleton. The age of a person and by studying the bones you can find a person’s age. Forensics Anthropology, (2014)
In order to grasp the concept of social construction of gender, it is essential to understand the difference between sex and gender. Biologically, there are only two reproductive genital organs that are determinants of sex: the vagina and the penis. Sex is established solely through biological structures; in other words, genitalia are the basis of sex. Once a sex category is determined, gender, a human categorization socially attached to sex, is assigned based on anatomy. Gender typically references social or cultural differen...
Human beings have been, and always will be, dichotomized into either male or female. When determining a person’s sex we often look for differences in facial features, body shape or mannerism’s, but another promising way to determine a persons sex and one that is most often used today, is through gender roles. Gender roles are behaviors that portray masculinity or femininity. The theory behind gender roles through multidisciplinary viewpoints is the focus of this paper. Throughout history and in every culture these roles have shifted and transformed into what society says is expectable. In this analysis, gender roles will be examined through a sociological, biological and evolutionary scope.
The scientific and medical progress of DNA as been emense, from involving the identification of our genes that trigger major diseases or the creation and manufacture of drugs to treat these diseases. DNA has many significant uses to society, health and culture of today. One important area of DNA research is that used for genetic and medical research. Our abi...
Sex and gender are terms that are mixed up from day to day and seen as similarities rather than differences. Sex is what distinguishes people from being either male or female. It is the natural or biological variation between males and females (Browne, 1998). Some of these variations are genital, body hair, and internal and external organs. It is the make-up of chromosomes, men have one X and one Y chromosome and women have two X chromosomes, which are responsible for primary characteristics (Fulcher and Scott, 2003).