The Enzyme Catalase Experiment

1751 Words8 Pages
The Enzyme Catalase Experiment Aim A series of experiments involving the enzyme Catalase has been performed in order to determine some of the enzyme's properties. The enzyme had its reaction rate found in different conditions. Variation of enzyme concentration, variation of pH, variation of temperature, and the effect of different concentrations of inhibitors were all tested. Increasing the enzyme concentration increased the reaction rate. An optimum pH and temperature were found for the enzyme, outside of this optimum the reaction rate would be lower. As inhibitor concentration rose, the reaction rate fell. Introduction Virtually all of the complex biochemical reactions that take place in animals, plants, and micro organisms are regulated by enzymes. Most enzymes are Proteins. Each enzyme is able to catalyse only one type (or a small number) of chemical reactions. Enzymes may only catalyse reactions which can happen naturally; the substrates do not require the enzyme but the reaction is much faster in its presence. In 1965 a theory was created by biologists describing an 'induced fit' - where the structure of an enzyme is altered by its substrate, by the movement of charges and hydrophobic/hydrophilic interactions, so the substrate fits perfectly on the active site in such a way that its reaction can be catalysed. Once the reaction has ended, the enzyme returns to its original shape which uses up the least energy to hold together. It was the work by chemists on the strong and weak chemical bonds which allowed for this theory to be created. Catalase was discovered to produce this induced fit in the presence of Hydrogen Peroxide. Catalase promotes the breakdown of Hydrogen Peroxide (H2O2) into non-harmful products, Water and Oxygen by the equation: CATALASE 2H2O2 ¯ ¯ ¯ ¯ ¯ ¯ ¯ ¯ ¯ ¯ ¯ ¯ ¯>2H2O + O2 Model of catalase structure As can be seen in the diagram, the Catalase molecule is a very complex protein. Its structure is held together by a variety of bonds

More about The Enzyme Catalase Experiment

Open Document