The Effect of Temperature on Anaerobic Respiration of Yeast
We wanted to find the effect of temperature on anaerobic respiration
of yeast. We investigated how a mixture of yeast, water, sugar, and
flour were affected.
Diagram
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Apparatus
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1g of yeast
0.5g of sugar
10g of flour
6 measuring cylinders - to put the sachets in and the 20cm3 of water
Measuring cylinders to measure the water
Thermometers - to keep the same temperature in the water baths
Water baths - at 20ºC, 30ºC, 40ºC, 50ºC, 60ºC
Hot air oven - at 70ºC
Beakers - to use to put the yeast mixture in the cylinders.
Method
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We set up the apparatus as shown, and placed 1g of yeast, 0.5g of
sugar, and 10g of flour each into the beakers. Then we added 20cm3 of
water, measured with a measuring cylinder, and stirred the mixture
until there were no bubbles left in it, and it was a smooth paste.
Then the volume of the dough in each was 25cm3 and we poured it into 6
measuring cylinders. Then we placed the 4 cylinders in 10ºC, 30ºC,
40ºC, 50ºC and 60ºC in water baths at those constant temperatures,
maintained with a thermometer. Then we left a cylinder at room
temperature (20ºC) and the last in a hot air oven at 70ºC. Every five
minutes we took a measurement of the volumes in each measuring
cylinder, and took a record of it. We repeated this two times.
Hypothesis
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I predict that the cylinders above 20ºC will increase in the volume of
the mixture, and that the yeast in the 70ºC hot air oven will
increase, but will then stop rising. When yeast respires
anaerobically, bu...
... middle of paper ...
...d.
I hav creatd another experiment which investigates the best
temperatures at which yeast enzymes work best at. I want to
investigate further into what exactly is the yeast enzymes' optimum
temperature, as it was 101cm3 at 50C, but as I mentioned before - the
dough was at unevern temperatures, as it was hard to control it
without the special abilities of laboratory machines. I know that the
optimum temperature is between 40C and 50C.
Method
We will mix 1g of yeast, 0.5g of sugar, and 10g of flour, each in 4
beakers with 20cm3 of water, which will be measured with a measuring
cylinder. Then we will pour it into measuring cylinders, but this time
using a measuring cylinder with a larger opening, to avoid spilling
the mixture around the edges of it. Then, the volumes in each of the 4
cylinders will be 25cm3
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So we can work out through this method that the volume of a box with
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