The Effect of Activator Concentration on the Rate of Reaction of Fungal Amylase
Aim
Investigate the effect of Activator concentration (Calcium) on the
rate of reaction of enzyme (fungal amylase) using Starch as a
substrate.
Introduction
Enzymes are made of globular proteins and each enzyme molecule is of a
particular complex shape complementary to the substrate, which it
breaks. The shape of the enzyme and its active site is due to the
specific folding of the polypeptides chain within it. Enzymes behave
as biological catalyst. A biological catalyst is a substance that
breaks down large molecule of food into smaller ones, so that they can
be absorbed into the body's blood stream; however, they break down the
substance without being used them selves, hence known also as a
biological catalyst. They do so by binding themselves temporary to the
substrate on their active site that lowers the amount of energy needed
to spark a chemical reaction this energy is also known as the
activation energy.
Enzyme are subject to change by external stimuli, these stimuli may be
chemical e.g. pH, enzyme Concentration and Substrate concentration or
physical e.g. Heat or temperature. These stimuli can alter the overall
time it takes for the break down of a molecule for a given substance
this /time is otherwise known as the rate of reaction. Depending on
the type of the stimuli, concentration, volume or the temperature
which the enzyme is being subjected to the rate of reaction can be
decreased, increased or not be effected at all.
However, there are some factors, which directly affect the enzymes
structures amongst these are inhibito...
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The investigation could be carried out by using chloride ions instead
of calcium ions, this will enable us to conduct the experiment at
lower temperatures and also allow us to find the effect of the
activator more precisely as it will be easier to conduct the
investigation because the Buffer solution required is pH7.
Over all my investigation was fair and the desired results were
produces, my prediction was right therefore showing an increase in the
rate of reaction.
Bibliography
Title Author/s
* Essential AS Biology Glenn and Susan Toole
* Biological Science 1&2 2nd Edition N.P.O.Green, G.W.Stout,
D.J.Taylor
* Biology 3rd Edition Arms& Camp
CDs
Compton Interactive Encyclopaedia 1998 Edition
Internet
www.s-cool.co.uk
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called an active site. This active site is made by a few of the amino
enzyme. As I'm sure you are well aware, enzymes do not get used up in
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The pH of the solution would alter the rate of the reaction if it was
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If I was to do this experiment again I might use a Fungi amylase to