The Effect Of Peroxidase

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Title
The Effects of Temperature, pH and Concentration on Enzyme.
Introduction
A catalyst is a substance that regulates and accelerate the rate of a biochemical reaction but is not used up or changed in those reactions. Enzymes are globular proteins that act as biological catalysts. Enzymes cannot make a reaction occur they just speed up the reaction from a 100,000times faster to over a billion times faster (Brooker 2012). They speed up the reaction by lowering the activation energy – the amount of energy needed to push a reactant to the level necessary for action. An enzyme activity is very chemically specific; some enzymes control only a specific reaction. In an Enzyme catalyzed reaction, the substance to which an enzyme acts on is called a substrate and the site to which the substrates fits is known as the active site (Marieb and Hoehn 2013). The process to which enzymes bind to substrate can be seen as lock and key. Only one key can open one lock.
Peroxidase is an enzyme which helps to promote the oxidation of various compounds using naturally occurring peroxides like hydrogen peroxide (H2O2) which is reduced to water. Peroxidase breaks down peroxides which are created by byproducts of biochemical reactions within organism, into harmless products.
H-R-O-H + H2O2 R=O + 2H2O
Guiacol Hydrogen peroxide Oxidized Guiacol
In this experiment, we looked at what will happen to peroxidase when placed in different pH, concentration and temperature. Peroxidase, an enzyme that catalyzes Hydrogen Peroxide reaction, will work best at pH range 5.0-9; will be slowed down in high temperatures and high concentrations.
Methods and Materials
Materials
Test tubes (50), Spectronic 20 (Spec. 20), Distilled water, Guaiacol, Hy...

... middle of paper ...

...mperature and pH on enzyme Amylase supports the hypothesis. From her result, it showed that Amylase worked faster at a certain pH range and worked best at 37°C temperatures (cite). A follow up experiment could be carried out to see what will happen if the concentration of the substrate were to change instead of that of the enzyme.

Reference
Brooker, Robert J. 2012."Overview of Genetics in Chapter 1 Concepts of Genetics. McGraw-Hill, New York. 2-5Pp.
"Effect of Temperature and PH on Amylase." Odinity. N.p., n.d. Web. 10 Apr. 2014
Fregeau,MR., M.Hoover, E.Maney [Eds].2012. Photosynthesis and Scicentific Wriritng, Chapter 8 in Biology 132 Introduction to Cells Lab Manual. Academx Publishing, Virginia Beach VA. 138pp.
Marieb, Elaine N., Hoehn,Katja. 2013. “Chemistry Comes Alive in Chapter 2 Human Anatomy and Physiology. Pearson Education, Inc. Glenview,IL. 50Pp

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