The Effect Of A Temperature Increase On The Breathing Rate Of A Locus
Introduction
This investigation will attempt to prove, whether or not a change in
temperature will affect the respiration rate (and therefore the
breathing rate) of a locust. Locusts are 'poikilothermic' and I will
be taking this factor into account when predicting the locust's
reaction to a temperature change.
Preliminary Testing
Preliminary tests consisted of a 'mock experiment', a kind of trial
run of the real thing. The test was carried out as follows:
· Fill a beaker with water of the desired temperature
· Place the locust in a boiling tube and place this in the water
· Observe the number of times the locust's abdomen moves up and down
and record the breathing rate over a period of time
· Repeat for different temperatures
These 'basic' preliminary tests uncovered a few 'flaws' in the
experiment and helped to highlight a few aspects that needed careful
consideration. Aspects that will need to be altered are, the length of
time left for the locust to acclimatise to the new temp. and the
length of time left for the locust to recover. Other factors that will
need consideration are, whether or not to take the locust out of the
tube to monitor its breaths, and over how long should its breathing be
monitored?
It was decided that the locust would need 3 minutes to acclimatise to
the new temperature and that 4-5 minutes would be sufficient time for
it to recover after being subjected to a change in temp. A period of
acclimatisation is required because preliminary tests showed that
locusts took an average of 3 minutes for their breathing rate to be
affected by the temperature change. A period of recovery is required
because it took an average of 4 minutes, 30 seconds to return their
breathing rate to normal
Research into 'Locusts' showed they breathe through their tracheae,
and a movement of their abdomen draws air in and pushes it out. This
means that, in order to count the number of breaths the locust takes,
Madagascar hissing cockroaches (Gromphadorhina portentosa) were the ectotherms used to compare standard metabolic rates and mass specific metabolic rates between organisms. To calculate metabolic rates for these individuals a system comprised of many parts was needed. A gas pump was needed to deliver airflow into the system. This gas pump was connected to a flow meter that could detect the flow rate of the gas passing through. The air would then flow into a Ascarite Column that would scrub out the CO2 from the system before the animal chamber was reached so that no CO2 that was not emitted by the animal would be collected. Then the Madagascar hissing cockroach would be in the animal chamber connected to the Ascarite Column and it would
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be too hot or too cold, this is a safety precaution for me as well as
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- Temperature was measured after and exact time i.e. 1 minute, 2 minutes, 3 minutes.
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Dudley, Robert. "The Evolutionary Physiology of Animal Flight: Paleobiological and Present Perspectives". Annual Review of Physiology. 2000. 63:135-55. 27 Aug 2007 http://arjournals.annualreviews.org