The Concentration of Substrate's Effect on the Rate of an Enzyme Controlled Reaction
Plan
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In this investigation I aim to find out whether the concentration of
hydrogen peroxide affects the rate at which catalyse breaks it down.
The rate of reaction will be measured by how much oxygen is collected.
Hydrogen Peroxide -------------------Water + Oxygen
---------------------------------------------------
2H O------------------- 2H O+ O
Experiment List
===============
* Potato
* Potato Borer
* Knife
* Ruler
* Hydrogen Peroxide
* Plastic Tub
* Water
* Conical Flask
* Rubber Bung
* Measuring Cylinder
* Stop Watch
Method
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Using potato borer, bore out 2 cylinder shaped pieces of potato.
Using a ruler and knife, chop the potato cylinders into 10 1cm pieces.
Measure out 50ml of hydrogen peroxide and pour into a conical flask.
Fill a plastic tub, ½ full of water.
Fill a measuring cylinder to the brim with water. Cover the end of the
measuring cylinder making sure no water escapes. Tip the measuring
cylinder upside down and place it into the plastic tub of water.
Take the rubber bung and tubing. Put the tubing so that the end is
inside the measuring cylinder in the water.
Put 10 pieces of potato in the hydrogen peroxide.
Place the rubber bung in the conical flask as soon as possible. Start
the stopwatch at the same time.
The oxygen produced by the potatoes and the hydrogen peroxide should
travel down the tubing into the measuring cylinder.
Using the scale on the measuring cylinder, it is possible to see how
much oxygen is being produced.
Take a reading every minute.
Repeat the experiment but with different volumes of hydrogen peroxide.
50ml H O (hydrogen peroxide)
40ml H O + 10ml H O -(water)
30ml H O + 20ml H O
How Amylase Concentration Affects the Rate of the Starch Concentration In this piece of coursework, I have to carry out an investigation to find out how amylase concentration affects the rate of starch. Enzymes are biological catalysts that speed up the chemical reaction that goes on inside living things. An enzyme acts on substrate and may do its job inside or outside the cells. However, the rate at which enzymes work are affected by the following factors/variables: Concentration:
Method: [IMAGE] Equipment needed: Ruler Measuring Cylinder Scalpel Tongs Pipette Thermometer Tri-pod Stop-clock Gauze Delivery Tube Bunsen Burner Beaker Matches/Lighter Potato Hydrogen Peroxide Solution (20%) Water Lead Nitrate The skin of the potato was removed using a scalpel and then cut into 1cm², using a ruler to measure the size of each cube, four cubes are required for each experiment, and therefore at least 36 cubes are required for the full experiment to take place. Fill a beaker half way with water, and place a thermometer in the water. Allow the thermometer to warm to room temperature to gather an accurate reading, and measure the temperature, using the thermometer. A measuring cylinder was used to put 10ml of Hydrogen Peroxide Solution into a
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
4. Pour about 300mL of tap water into the beaker. Set up a hot-water bath using a hot plate, retort stand, and thermometer clamp. Alternatively, use a Bunsen burner, retort stand, ring clamp, thermometer clamp, and wire gauze.
This enzyme speeds up the break down of hydrogen peroxide into water and oxygen, as enzymes are biological catalysts. [IMAGE]The reaction: Hydrogen peroxide Water + Oxygen Catalase -------- [IMAGE] 2H2O2 2H2O + O2 Apparatus: Hydrogen Peroxide, Several sticks of celery, Stand, boss and clamp, 100ml conical flask, 25cm3 burette, 1800cm3 beaker, Rubber bung with delivery tube, Distilled water, Large container filled with water, 10cm3 measuring cylinder, 10cm3 syringe, 20cm3 syringe, Blender, Knife, Ceramic tile, Electronic balance (correct to 2 decimal places), Sieve, Stopwatch/timer. The variables: There are many possible variables in this investigation, such as pH, temperature, the concentration of substrate and the concentration of the enzyme.
== == == = This is what I'm going to be changing in the experiment and this will be the temperature and the concentration of the yeast. There are several variables in this experiment, they are: · Amount Used - Too much or too little of the hydrogen peroxide causes the reaction to speed up/slow down producing different amounts of oxygen.
The Effect of a Catalase on the Breakdown of Hydrogen Peroxide Aim To follow the progress of a catalysed reaction by measuring the volume of gas produced as the reaction proceeds. Using the initial rates of a series of experiments I will be able to find the orders of the reaction with respect to enzyme and substrate. Also to find out if concentration has an effect on the reaction when an enzyme is used to accelerate the breakdown of hydrogen peroxide.
It is important however to note that the NH4 and K ions are still in
is a very useful nutrient for human life forms as it is the source for
Start with the hot water and first measure the temperature. Record it. 8. Then pour 40 ml into the beaker. You can measure how much water was used by looking at the meniscus.
Observations: Liver and Potatoes will be placed in hydrogen peroxide in order to observe the reactions due to the enzyme, catalase, found in both.
We then cut our potato tubes with the cork borer and cut them with the scalpel so they were the same length and weighed them. We then put one potato tube in each test tube and then added the same amount sugar solution in to each tube. The concentration of sugar solutions varied in each test tube.
I shall be measuring how much gas is given off. This will be done by measuring the amount of froth on the surface of the liquid. The oxygen released is collected in the form of these bubbles. The equation for the reaction is: (catalase) [IMAGE] H2O2 2H2O + O2 (hydrogen peroxide) (2 part water) (oxygen) I will change the concentration of H2O2 and O2 (making sure the volume stay the same, when one part of a H2O2 particle is taken, an O2 particle is added. Prediction
The Concentration of Hydrogen Peroxide and Speed of The Rate at Which It is Broken Down by Catalase
Equipment Potato, Borer, Beakers, Measuring Cylinder, Stopclocks, Distilled Water, Electronic Balance, Salt solutions of various concentrations. Diagram [IMAGE] [IMAGE] [IMAGE] When we leave the potato in the solution for the allocated time, water