Testing Tasting Phenotype And Genotypes

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Phenylthiocarbamide is used to test tasting phenotype and genotypes. In 1931 a hypothesis was formulated stating that at least one bitter receptor is sensitive to PTC but is also inactive in some individuals [Newcomb R.D, 2012]. PTC concentration paper of different concentration was used in this experiment to test the ability to taste PTC amongst genetic lab students. DNA was extracted from cheek cells, amplified with PCR and digested with HaeIII.
The purpose of this experiment is to compare the ability of students in tasting PTC. The hypothesis formulated for genotypes states that there is a significant difference between tasters and non- taster, with the null hypothesis stating that there is no significant difference between taster and non- taster. During the phenotypic observation two different hypothesis were formulated as well, there is significant difference between the three observed phenotype and the null hypothesis states that there is no significant difference between the three phenotype. Through observation and calculation it is concluded that there is a significant difference between the Genotypes. On the other, there is no significant difference between the phenotype.
Introduction:
Taste has been divided into 5 different categories. Humans are able to taste sweet, sour, bitter, salty and umami. The ability to taste PTC has been viewed as a dominant and recessive trait that varies amongst the human population. The gene for the PTC taste receptor, which is TAS2R38, was discovered in 2003. In 1931 a hypothesis was formulated stating that out of the bitter receptors at least one is sensitive to PTC but is inactive in some people [Newcomb R.D, 2012]. The ability to taste PTC was discovered by Arthur Fox. While working...

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...l 24 24 Chi 2 =1.75

Table 3.
Genotype Observed Expected O-E (O-E)2 (O-E)2 /E
Taster 18 12.5 5.5 30.25 2.42
Non taster 7 12.5 -5.5 30.25 2.42
Total 25 25 Chi 2 = 4.84

Discussion: In calculating the Phenotypes one of the students did not have any band present which cause the data for the phenotype to be only out of 24. There is an assumption that subject is either homozygous dominant or heterozygous dominant because of the information that the individual was able to taste PTC in both low and in high concentration. With the results obtained the null hypothesis was accepted, meaning that there are no significant difference between the ability to taste PTC amongst the three phenotypes, which is proven in table 2. With the genotype testing we reject the null hypothesis, which in turns means there is a significant difference between Taster and non- taster.

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