1. Introduction
The scaling is a technique for propagation of Lilium (Gray, 1974; Marinangeli and Curvetto, 1997; Marinangeli et al., 2003). The mother bulbs are scaled and stored in a favorable substrate. Scale bulblets develop on the sides of the individual mother scale (Gray, 1974). This method was studied and reported earlier (Matsuo, 1972; Matsuo and Van Tuyl, 1984; Matsuo et al., 1989; Cabrera, 2002). Addai (2010) found the sucrose as an essential and major carbohydrate in Lilium bulb, because breaking and metabolism of this sugar are important to facilitate the growth of storage organ, inflorescence and others. However, Addai (2010) noticed that starch is a major carbohydrate and as an energy source for bulbous plants such as lily before vernalization. After vernalization, starch breaks to sucrose and reducing sugars (Xu et al., 2006), this phenomenon referred to low temperature sweetening, and has been reported in Lilium, Hyacinthus and Tulipa as well (Miller and Langhans, 1990). Sucrose is a soluble carbohydrate, which is essential for growth of plants, and is as first transfer carbohydrate in more plants. The accumulation of sucrose in scale tissue of bulb can cause sprouting and growing plant (Xu et al., 2006). After translocation of starch from chloroplast and amyloplast to cytosol, UDP-glucose is a start-point of sucrose synthesis. Thus, glucose is a molecule key in synthesizing sucrose, and also glucose is a photosynthesis product its own, and with other photosynthetic product, fructose, produce a sucrose. Addai (2010) described that metabolism of sucrose, glucose and starch supply necessary energy to sprout and the emergence of shoot and roots, also he noticed that fructose accumulate in scales and has a role in spr...
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...ter lily bulbs. HortScience 25(4): 463-465.
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Arabidopsis thaliana (L.) Columbia ecotype suspension- cultured T87 cells were maintained at 22°C in JPL3 medium with continuous illumination and shaking at 100g. Two-week-old cells were sieved through 500 μm stainless mesh and the remaining filtrate was transferred to a flask containing 20 ml of fresh JPL3 medium for subculture.
The independent variable for this experiment is the enzyme concentration, and the range chosen is from 1% to 5% with the measurements of 1, 2, 4, and 5%. The dependant variable to be measured is the absorbance of the absorbance of the solution within a colorimeter, Equipments: Iodine solution: used to test for present of starch - Amylase solution - 1% starch solution - 1 pipette - 3 syringes - 8 test tubes – Stop clock - Water bath at 37oc - Distilled water- colorimeter Method: = == ==
With this information we were able to identify any patterns and similarities. Hypothesis: The higher the temperature of water, potato and H²O², the rate at which the Enzyme will work will be faster therefore producing more oxygen. The reaction will be the same without the catalase (potato). Therefore in both experiments the Enzyme will work more rapidly and produce more oxygen. Aim: To test the hypothesis.
Both starch and sucrose can be converted back into glucose and used in respiration. Photosynthesis happens in the mesophyll cell of leaves. There are two kinds of mesophyll cells - palisade mesophyll and spongy mesophyll. The mesophyll cells contain tiny bodies called chloroplasts which contain a green chemical called chlorophyll.
The temperature of amylase The temperature of starch Room temperature Concentration Ph values The variable I will be changing is the volume of amylase. Safety: The sand is To make sure I carry out this experiment safely I will make sure I wear a pair of goggles. I will ensure I keep my stool under the table and all
[IMAGE]Carbon dioxide + water Light Energy glucose + oxygen Chlorophyll [IMAGE]6CO2 + 6H20 Light Energy C6 H12 O6 + 6O 2 Chlorophyll Photosynthesis occurs in the leaves of the plant in the palisade layer. Chlorophyll in the cells in the palisade layer absorb light for photosynthesis. The plant releases the oxygen created in photosynthesis back into the air but it uses or stores the glucose for energy, respiration, growth and repair. The leaves and plants are also specially adapted for photosynthesis in their structure and cell alignment. Preliminary Experiment Apparatus * Piece of Elodea Canadensis * Bulb * Voltmeter * Test tube * Beaker * Box *
The cultures were maintained at 25±20C under 16 hr illumination of 4000 lux intensity. The results are presented in Table 1, it can be seen from the data that pH of the medium had significant effect not only on regeneration frequency but also on number of shoots developed in each culture. Maximum 62.5±4.7 percent cultures in CoS 98259 and 67.3±4.9 percent in CoS 767 developed shoots at pH 6.0 while regeneration frequency was the lowest at pH 5.6. An increase in pH form 6.0 to 6.2 and 6.4 reduced the frequency of shoot regeneration from the callus (Table
Two members of the group were instructed to visit the laboratory each day of the experiment to water and measure the plants (Handout 1). The measurements that were preformed were to be precise and accurate by the group by organizing a standardized way to measure the plants. The plants were measured from the level of the soil, which was flat throughout all the cups, to the tip of the apical meristems. The leaves were not considered. The watering of the plants took place nearly everyday, except for the times the lab was closed. Respective of cup label, the appropriate drop of solution was added to the plant, at the very tip of the apical meristems.
The primary nutrients that plants require are carbon, hydrogen, and oxygen from air and water. Sugar has a different effect on animals, humans, and plants. In this experiment, we are testing the effects of sugar on the growth of wheatgrass. For this experiment, we used regular sugar that we put in our food and drinks. To test our hypothesis we are using two groups control and experiment group. The control plant received only water but the experimental plant received sugar but, both plants were placed in the same temperature and same amount of water. Our hypothesis was correct, experiment plant “sugar water” yield more plant growth than control plant “water”. This experiment shows the sugar water plant grow faster than water because of the average of both plants. The sugar water experiment plant had longer in length compared to water control plant.
Author Unknown (Editorial) 2005 Reburnishing [sic] Golden Rice. Nature Biotechnology. Vol. 23. No. 4. Pp. 395. April
The “Fast Plant” experiment is an observation of a plants growth over the span of twenty-eight days. The objective is to observe how plants grow and use their resources throughout the span of their life. In our lab we observed the Brassica rapa, a herbaceous plant in the mustard family which has a short cycle which makes it a perfect plant to observe in this experiment. Like other plants the Brassica rapa must use the resources in the environment to create energy to complete itʻs life cycle and reproduce. By observing the plant it is easy to see in what organ or function the plant is using itʻs energy and resources and if overtime the resources switch to other part of the plants. By conducting this experiment we are able to observe where and how plants allocate their resources throughout their life by harvesting plants at different points in their life.
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
However, the decrease varied depending on the temperature. The lowest temperature, 4 degrees Celsius, experienced a very low decrease of amylose percentage. Temperature at 22 degrees Celsius and 37 degrees Celsius, both had a drastic decrease in amylose percentage. While the highest temperature, 70 degrees Celsius, experienced an increase of amylose percentage. In conclusion, as the temperature increases the percentage of amylose decreases; however, if the temperature gets too high the percentage of amylose will begin to increase. The percentage of amylose increases at high temperatures because there is less enzyme activity at high temperatures. However, when the temperature is lower, more enzyme activity will be present, which results in the decrease of amylose percentage. This is why there is a decrease of amylose percentage in 4, 22, and 37 degrees Celsius. In this experiment the optimal temperature is 37 degrees Celsius, this is because this is the average human body temperature. Therefore, amylase works better at temperatures it is familiar
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells
Plant nutrition is area of plant biology that is of the utmost importance for the proliferation of plants. Without proper nutrition, plants would simply cease to exist unless drastic alterations were made. There are certain elements that are required for the plant to grow and reproduce; these elements are known as essential elements. There are three requirements of an essential element: the element must be required for the completion of the plant’s life cycle, the element must not be replaceable by another element in whole, and finally the element must be direction involved in the metabolism of the plant. Chemical compounds that are involved in proper nutrition have been designated as nutrients, and further classified as macronutrients and micronutrients. Macronutrients are needed for growth, metabolism, and many other functions, but are designated as “macro” because they are required in larger amounts. Macronutrients include carbohydrates, proteins, and fat molecules. Micronutrients have a much wider function that depends on the exact micronutrient. Micronutrients are designated as so because they are needed in much smaller amounts when compared to macronutrients. Examples of micronutrients include vitamins and minerals.