Sterilization by Saturated Steam

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Title: Sterilization by Saturated Steam
Introduction:
Sterilization process is done for the complete elimination of all microorganisms that could potentially be a contaminant and lead to occurrences of hazards. (Chi, 1993) Sterilization is a process defined by killing all the living microorganisms that includes bacteria and spores. This process can be done chemically, physically and by means of physiochemical methods. (Soper & Davies, 1990)There are chemicals used in these processes to ensure all microorganisms no longer exist and they are known as sterilizing agents and are called chemisterilants. The result of sterilization often depends on the type of substance being sterilized and the conditions made to suitably sterilize it. (Soper & Davies, 1990)
The types of sterilization methods differs from the usage of saturated steam under pressure or by using heat which can be categorized into moist and dry heat. Usage of heat is considered to be the oldest and most reliable method of sterilization. A few other ways of sterilization include ionizing radiation, filtration and the input of gas.
DQ2Moist heat sterilization can be carried out by exposing microorganisms to saturated steam under pressure in an operating machine called the autoclave. Once the microorganisms are being sealed in the autoclave, their enzymes and structural components of proteins will start changing and the changes formed are not reversible. (Yesair, 1946) As the temperature in the autoclave increases, the amount of water in the autoclave will decrease. This method of sterilization requires accurate time, temperature and pressure measurement. Cleanliness and removal of any debris found in the autoclave machine should be removed. When initiating this method, air ...

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